Synergistic anti-inflammatory and antioxidant dietary supplement compositions
A dietary supplement and synergistic technology, applied in the direction of food ingredients as antioxidants, food science, drug combination, etc., can solve problems that have not been involved
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example 1
[0135] Synergistic inhibition of 5-lipoxygenase activity:
[0136] The ability of the novel dietary supplement composition to inhibit 5-lipoxygenase was detected by a colorimetric method. The assay mixture contains 50 mM phosphate buffer (pH 6.3), 5-lipoxygenase, dimethyl sulfoxide and linolenic acid solution in a total volume of 0.5 mL, containing various concentrations of the composition to be tested (5 μg, 10 μg, 20 μg), after the above reaction mixture was mixed for 5 minutes, 0.5 mL of iron xylenol orange agent was added, and the OD value at 585 nm was detected by a spectrophotometer two minutes later. Then change the solution of the composition to be tested into the carrier and measure the control value again according to the above method. Percent inhibition was calculated from the absorbance of the test composition and the absorbance of the control.
[0137] The results are shown in the table below
[0138] Table 1. Inhibition of 5-Lipoxygenase Activity
[0139] ...
example 2
[0143] Antioxidant activity of the composition:
[0144] The antioxidant activity of the dietary supplement composition was detected by superoxide radical scavenging method. The superoxide radical scavenging activity is determined by NBT (nitro blue tetrazolium) method. Reaction mixture contains EDTA (6.6mM), NaCN (3 μ g), riboflavin (2 μ M), NBT (50 μ M), and total volume is 3ml ethanol solution, contains the composition to be tested of various concentrations and phosphate buffer saline ( 58mM, pH 7.8). Optical density at 560 nm was detected. After the test tube was uniformly irradiated under an incandescent lamp for 15 minutes, the optical density at 560 nm was detected again. The percent inhibition and the percent generation of peroxide groups were calculated from the absorbance of the composition to be tested and the absorbance of the control group.
[0145] The antioxidant activity of the dietary supplement composition was detected by DPPH method. The DPPH (1,1-diphe...
example 3
[0152] Synergistic anti-inflammatory activity of Composition-1: The test animals (rats, 200-300 g) were divided into random groups, 5 animals in each group, irrespective of male or female. The diet of mice was supplemented with demethyl curcumin (50 mg / kg) or concentrated AKBA extract (50 mg / kg) or composition 1 (50 mg / kg) per 1% CMC for 28 consecutive days. The control group received the same volume of vehicle (1% CMC), a standard drug, prednisolone (10 mg / kg, orally). On day 14, 14,100 μl of Freund's complete adjuvant (FCA) was subcutaneously injected into the implanted area of the left hindlimb. Blood samples from each animal were collected on the day before the FCA challenge and on the day the test was terminated, ie, on the 14th day after the challenge. On day 28, paw volumes were measured and animals were dissected and liver tissue excised and kept in storage at -80°C. Measure pro-inflammatory indicators, such as nitrite, serum IL-Iβ, and paw edema.
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