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Synergistic anti-inflammatory and antioxidant dietary supplement compositions

A dietary supplement and synergistic technology, applied in the direction of food ingredients as antioxidants, food science, drug combination, etc., can solve problems that have not been involved

Inactive Publication Date: 2010-03-31
LAILA NUTRACEUTICALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0043] There is therefore nothing in the prior art concerning a composition containing concentrated AKBA extract and concentrated desmethylcurcumin which has a synergistic inhibitory effect on 5-lipoxygenase

Method used

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  • Synergistic anti-inflammatory and antioxidant dietary supplement compositions
  • Synergistic anti-inflammatory and antioxidant dietary supplement compositions
  • Synergistic anti-inflammatory and antioxidant dietary supplement compositions

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0135] Synergistic inhibition of 5-lipoxygenase activity:

[0136] The ability of the novel dietary supplement composition to inhibit 5-lipoxygenase was detected by a colorimetric method. The assay mixture contains 50 mM phosphate buffer (pH 6.3), 5-lipoxygenase, dimethyl sulfoxide and linolenic acid solution in a total volume of 0.5 mL, containing various concentrations of the composition to be tested (5 μg, 10 μg, 20 μg), after the above reaction mixture was mixed for 5 minutes, 0.5 mL of iron xylenol orange agent was added, and the OD value at 585 nm was detected by a spectrophotometer two minutes later. Then change the solution of the composition to be tested into the carrier and measure the control value again according to the above method. Percent inhibition was calculated from the absorbance of the test composition and the absorbance of the control.

[0137] The results are shown in the table below

[0138] Table 1. Inhibition of 5-Lipoxygenase Activity

[0139] ...

example 2

[0143] Antioxidant activity of the composition:

[0144] The antioxidant activity of the dietary supplement composition was detected by superoxide radical scavenging method. The superoxide radical scavenging activity is determined by NBT (nitro blue tetrazolium) method. Reaction mixture contains EDTA (6.6mM), NaCN (3 μ g), riboflavin (2 μ M), NBT (50 μ M), and total volume is 3ml ethanol solution, contains the composition to be tested of various concentrations and phosphate buffer saline ( 58mM, pH 7.8). Optical density at 560 nm was detected. After the test tube was uniformly irradiated under an incandescent lamp for 15 minutes, the optical density at 560 nm was detected again. The percent inhibition and the percent generation of peroxide groups were calculated from the absorbance of the composition to be tested and the absorbance of the control group.

[0145] The antioxidant activity of the dietary supplement composition was detected by DPPH method. The DPPH (1,1-diphe...

example 3

[0152] Synergistic anti-inflammatory activity of Composition-1: The test animals (rats, 200-300 g) were divided into random groups, 5 animals in each group, irrespective of male or female. The diet of mice was supplemented with demethyl curcumin (50 mg / kg) or concentrated AKBA extract (50 mg / kg) or composition 1 (50 mg / kg) per 1% CMC for 28 consecutive days. The control group received the same volume of vehicle (1% CMC), a standard drug, prednisolone (10 mg / kg, orally). On day 14, 14,100 μl of Freund's complete adjuvant (FCA) was subcutaneously injected into the implanted area of ​​the left hindlimb. Blood samples from each animal were collected on the day before the FCA challenge and on the day the test was terminated, ie, on the 14th day after the challenge. On day 28, paw volumes were measured and animals were dissected and liver tissue excised and kept in storage at -80°C. Measure pro-inflammatory indicators, such as nitrite, serum IL-Iβ, and paw edema.

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Abstract

Disclosed herein are novel dietary supplement compositions comprising enriched 3-O-acetyl-11-keto-ss-boswellic acid and enriched demethylated curcuminoids, wherein the composition exhibits anti-inflammatory, antiulcerogenic and antioxidant activities. Also disclosed are novel compositions comprising enriched 3-0-acetyl-ll-keto-ss- boswellic acid, enriched demethylated curcuminoids that have a synergistic effect on specific inhibition of- COX-2 and 5-LOX activity and optionally containing glucosamine, resveratrol, garlic extract, chondroitin, methylsulphonylmethane, bromelain, serratiopeptidase,quercitine, gallic acid, caffeic acid, green tea extract, aspirin and ibuprofen.

Description

technical field [0001] The present invention relates to those synergistic compositions useful for the treatment and prevention of lipoxygenase-mediated, free radical-mediated disorders and various musculoskeletal degenerative diseases, such as rheumatoid arthritis and osteoarthritis, in mammals. The present invention also relates to those compositions which have a synergistic inhibitory effect on inducible cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX). These compositions preferably contain a concentrated 3-O-acetyl-11-keto-β-boswellic acid (AKBA), concentrated demethylcurcumin, and optionally other Element. Background of the invention [0002] Inflammatory diseases affect more than half a million Americans. Basic research into molecular and cellular immunity over the past ten to fifteen years has led to marked changes in the way these diseases of the immune system are diagnosed, treated, and prevented. One such example was the discovery of an inducible cyclooxygenas...

Claims

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Application Information

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IPC IPC(8): A23L1/30A61K31/19A61K31/12
CPCA23V2002/00A61K31/12A61K31/19A23L1/3002A23L33/105A61P25/16A61P25/28A61P29/00A61P35/00A61P9/00A61K2300/00A23V2200/306A23V2200/312A23V2200/314A23V2200/32A23V2200/308A23V2200/326A23V2250/21A23V2250/2112A23V2250/308A23V2200/02A23V2250/2132A23V2250/212
Inventor 刚迦·拉贾·高卡拉贾楚莫图鲁·高拉考提维达特斯瓦鲁·萨姆帕利
Owner LAILA NUTRACEUTICALS
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