Organic/inorganic hybridized microcapsule prepared in biomimetic way by simulated body fluid method and preparation method thereof
A technology for simulating body fluids and microcapsules, applied in biochemical equipment and methods, chemical instruments and methods, microsphere preparation, etc., can solve the problems of expensive and toxic precursors, and improve mechanical strength and operational stability. Good reproducibility and mild preparation conditions
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Embodiment 1
[0021] Preparation containing simulated body fluid (I), the specific composition is: 134.0mmol / L Na + , 5.0mmol / L K + , 30.0mmol / LCa 2+ , 3.0mmol / L Mg 2+ , 258.0mmol / L Cl - , 0.5mmol / L SO 4 2- . Prepare simulated body fluid (II), the specific composition is 350.0mmol / L Na + , 5.0mmol / L K + , 8.4mmol / L HCO 3 - , 148.0mmol / L Cl - , 50.0mmol / LHPO 4 2- , 0.5mmol / L SO 4 2- .
[0022] Sodium alginate was dissolved in simulated body fluid (II) to prepare a solution containing 1.0% sodium alginate mass fraction. Chitosan is dissolved in simulated body fluid (I) to prepare a solution containing chitosan with a mass fraction of 0.5%. At room temperature, use a 5ml syringe with a needle tube diameter of 0.5mm to drop the prepared sodium alginate solution in the chitosan solution, and the small droplet is taken out after chitosan is dissolved in the simulated body fluid (I) and preserved for 20 minutes, and then Rinse in distilled water. All steps were performed at room ...
Embodiment 2
[0024] Preparation containing simulated body fluid (I), the specific composition is: 134.0mmol / L Na + , 5.0mmol / L K + , 60.0mmol / LCa 2+ , 3.0mmol / L Mg 2+ , 258.0mmol / L Cl - , 0.5mmol / L SO 4 2- . Prepare simulated body fluid (II), the specific composition is 350.0mmol / L Na + , 5.0mmol / L K + , 8.4mmol / L HCO 3 - , 148.0mmol / L Cl - , 100.0mmol / L HPO 4 2- , 0.5mmol / L SO 4 2- .
[0025] Sodium alginate was dissolved in simulated body fluid (II) to prepare a solution containing 2.0% sodium alginate mass fraction. Chitosan was dissolved in simulated body fluid (I) to prepare a solution containing chitosan with a mass fraction of 0.8%. At room temperature, the prepared sodium alginate solution was added dropwise to the chitosan solution with a 5ml syringe with a needle diameter of 0.9 mm to prepare polysaccharide microcapsules biomimetic mineralized with hydroxyapatite. The small droplets were removed after 30 min in chitosan dissolved in simulated body fluid (I) and r...
Embodiment 3
[0027] Preparation containing simulated body fluid (I), the specific composition is: 134.0mmol / L Na + , 5.0mmol / L K + , 120.0mmol / LCa 2+ , 3.0mmol / L Mg 2+ , 258.0mmol / L Cl - , 0.5mmol / L SO 4 2- . Prepare simulated body fluid (II), the specific composition is 350.0mmol / L Na + , 5.0mmol / L K + , 8.4mmol / L HCO 3 - , 148.0mmol / L Cl - , 200.0mmol / L HPO 4 2- , 0.5mmol / L SO 4 2- .
[0028]Sodium alginate was dissolved in simulated body fluid (II) to prepare a solution containing 3.0% sodium alginate mass fraction. Chitosan was dissolved in simulated body fluid (I) to prepare a solution containing chitosan with a mass fraction of 1.2%. At room temperature, the prepared sodium alginate solution was added dropwise to the chitosan solution with a 5ml syringe with a needle diameter of 1.5 mm to prepare polysaccharide microcapsules biomimetic mineralized with hydroxyapatite. Droplets were removed after 50 min of chitosan dissolved in simulated body fluid (I) and rinsed in d...
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