A method for continuous extraction of chlorella functional components

A functional ingredient, the technology of Chlorella, which is applied in the preparation methods of peptides, chemical instruments and methods, algae/moss peptides, etc., can solve the problems of unclear products, long operation cycle, low yield, etc., and achieve simple operation. Reliable, easy to maintain active effect

Inactive Publication Date: 2011-12-07
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] CN1018071B has introduced a kind of ecological harvesting method of chlorella, but the operation cycle is too long, the yield is low, and affects the normal growth of chlorella
CN1302013C introduces a preparation method of active chlorella polysaccharides, which breaks the wall by ultrasonic waves, and then extracts and removes impurities by hot leaching. functions such as cerebrovascular diseases, but its yield is low, and other functional components of chlorella are wasted
CN101053577A discloses the preparation method of chlorella pressure broken wall and nucleotide, protein, polysaccharide, algae dry powder, adopts normal temperature pressure broken wall to chlorella, but needs - 20 ℃ freezing in the process of preparing product, and its energy consumption very high
There is also the phenomenon that the product is not clear, the yield is low and the waste is serious

Method used

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  • A method for continuous extraction of chlorella functional components
  • A method for continuous extraction of chlorella functional components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Use a pump to introduce the chlorella culture solution into a membrane separation system using polysulfone (or ceramic or polyether) as the membrane material for microporous membrane filtration. The control membrane separation process parameters are: cross-flow velocity 2.4cm / s, operating pressure The chlorella was concentrated to 0.5% of the original volume at a temperature of 0.02MPa and a temperature of 20°C to obtain a chlorella concentrate. Add cellulase 0.1%, pectinase 0.1%, and xylanase 0.1% to the concentrated solution (the amount of enzyme added is the percentage of dry matter in the chlorella concentrate), the temperature is 40℃, and the enzymatic hydrolysis is carried out for 1 hour Break the wall. A concentrated solution of Chlorella vulgaris was obtained. Ethanol was added to the concentrated solution of chlorella vulgaris to a concentration of 50% and a temperature of 50° C. for extraction for 2 hours. Then centrifuged (3000r / min) to obtain ethanol extract...

Embodiment 2

[0035] Use a pump to introduce the chlorella culture solution into a membrane separation system using polysulfone (or ceramic or polyether) as the membrane material for microporous membrane filtration. The process parameters of the membrane separation are controlled as follows: the cross-flow velocity is 5 cm / s and the operating pressure is The chlorella was concentrated to 0.8% of the original volume at 0.04 MPa, the temperature was 30°C, and the chlorella concentrate was obtained. Add cellulase 0.2%, pectinase 0.3%, and xylanase 0.4% to the concentrated solution (the amount of enzyme added is the percentage of dry matter in the chlorella concentrate), the temperature is 50℃, and the enzymatic hydrolysis is carried out for 2 hours Broken the wall to obtain a concentrated solution of broken wall chlorella. Add ethanol to the broken chlorella concentrate to make the concentration 60%, the temperature is 55°C, and the extraction is performed for 3 hours. Then centrifuged (3000r / ...

Embodiment 3

[0037] Use a pump to introduce the chlorella culture solution into a membrane separation system using polysulfone (or ceramic or polyether) as the membrane material for microporous membrane filtration. The process parameters of the membrane separation are controlled as follows: the cross-flow velocity is 4 cm / s and the operating pressure is The chlorella was concentrated to 1% of the original volume at 0.08 MPa, the temperature was 40°C, and the chlorella concentrate was obtained. Add cellulase 0.3%, pectinase 0.3%, and xylanase 0.3% to the concentrate (the amount of enzyme added is the percentage of the dry matter in the chlorella concentrate), the temperature is 60℃, and the enzymatic hydrolysis is carried out for 5 hours Broken the wall to obtain a concentrated solution of broken wall chlorella. Ethanol was added to the broken chlorella concentrate to make the concentration 70%, the temperature was 50°C, and the extraction was performed for 6 hours. After centrifugation (30...

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Abstract

A method for continuous extraction of functional components of chlorella, in which chlorella is enriched by membrane separation technology to obtain concentrated chlorella slurry, and appropriate enzymes are added to the liquid phase system to break the wall, and the broken chlorella Chlorella solution extracts chlorophyll with a solvent to obtain functional pigment products and reduce the color value of subsequent products; the decolorized chlorella is first enzymatically hydrolyzed in the water phase, and then the functional oil is extracted with a solvent to obtain defatted chlorophyll The active polysaccharides of the algae are extracted by an acid method, and the rest is dried to obtain the crude protein of the algae. The main products include chlorophyll, chlorella active polysaccharides, chlorella functional oils, chlorella protein, etc. On the basis of ensuring the activity of chlorella functional components, the process is optimized to obtain a higher yield and reduce Cost of production.

Description

Technical field [0001] The invention belongs to the technical field of marine biology, and particularly relates to a method for continuous extraction of chlorella functional components. Background technique [0002] Chlorella is a pervasive single-celled green algae, belonging to the Chlorella genus Chlorella, Oocystaceae, Chlorella, Chlorellae. It is widely distributed in nature and can use light energy to autotrophically and also use organic carbon sources under heterotrophic conditions. For growth and reproduction. There are about 15 species of Chlorella in the world, plus hundreds of varieties. Chlorella is rich in nutrients and biologically active substances. Usually chlorella has a high protein content (55% to 67%), contains more sugars (15% to 20%) and lipids (5% to 10%), and contains biologically active polysaccharides, growth factors, and glycoproteins , Glycolipoprotein, Dietary Fiber, etc. It contains a complete range of amino acids in protein, including eight esse...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C09B61/00C08B37/00C07K14/405C07K1/14
Inventor 刘贺朱丹实何余堂郭晓飞刘昊东
Owner BOHAI UNIV
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