A method for continuous extraction of chlorella functional components

Abstract

A method for continuous extraction of functional components of chlorella, in which chlorella is enriched by membrane separation technology to obtain concentrated chlorella slurry, and appropriate enzymes are added to the liquid phase system to break the wall, and the broken chlorella Chlorella solution extracts chlorophyll with a solvent to obtain functional pigment products and reduce the color value of subsequent products; the decolorized chlorella is first enzymatically hydrolyzed in the water phase, and then the functional oil is extracted with a solvent to obtain defatted chlorophyll The active polysaccharides of the algae are extracted by an acid method, and the rest is dried to obtain the crude protein of the algae. The main products include chlorophyll, chlorella active polysaccharides, chlorella functional oils, chlorella protein, etc. On the basis of ensuring the activity of chlorella functional components, the process is optimized to obtain a higher yield and reduce Cost of production.

Description

Technical field

[0001] The invention belongs to the technical field of marine biology, and particularly relates to a method for continuous extraction of chlorella functional components. Background technique

[0002] Chlorella is a pervasive single-celled green algae, belonging to the Chlorella genus Chlorella, Oocystaceae, Chlorella, Chlorellae. It is widely distributed in nature and can use light energy to autotrophically and also use organic carbon sources under heterotrophic conditions. For growth and reproduction. There are about 15 species of Chlorella in the world, plus hundreds of varieties. Chlorella is rich in nutrients and biologically active substances. Usually chlorella has a high protein content (55% to 67%), contains more sugars (15% to 20%) and lipids (5% to 10%), and contains biologically active polysaccharides, growth factors, and glycoproteins , Glycolipoprotein, Dietary Fiber, etc. It contains a complete range of amino acids in protein, including eight esse...

Examples

Embodiment 1

[0033] Use a pump to introduce the chlorella culture solution into a membrane separation system using polysulfone (or ceramic or polyether) as the membrane material for microporous membrane filtration. The control membrane separation process parameters are: cross-flow velocity 2.4cm / s, operating pressure The chlorella was concentrated to 0.5% of the original volume at a temperature of 0.02MPa and a temperature of 20°C to obtain a chlorella concentrate. Add cellulase 0.1%, pectinase 0.1%, and xylanase 0.1% to the concentrated solution (the amount of enzyme added is the percentage of dry matter in the chlorella concentrate), the temperature is 40℃, and the enzymatic hydrolysis is carried out for 1 hour Break the wall. A concentrated solution of Chlorella vulgaris was obtained. Ethanol was added to the concentrated solution of chlorella vulgaris to a concentration of 50% and a temperature of 50° C. for extraction for 2 hours. Then centrifuged (3000r / min) to obtain ethanol extract...

Embodiment 2

[0035] Use a pump to introduce the chlorella culture solution into a membrane separation system using polysulfone (or ceramic or polyether) as the membrane material for microporous membrane filtration. The process parameters of the membrane separation are controlled as follows: the cross-flow velocity is 5 cm / s and the operating pressure is The chlorella was concentrated to 0.8% of the original volume at 0.04 MPa, the temperature was 30°C, and the chlorella concentrate was obtained. Add cellulase 0.2%, pectinase 0.3%, and xylanase 0.4% to the concentrated solution (the amount of enzyme added is the percentage of dry matter in the chlorella concentrate), the temperature is 50℃, and the enzymatic hydrolysis is carried out for 2 hours Broken the wall to obtain a concentrated solution of broken wall chlorella. Add ethanol to the broken chlorella concentrate to make the concentration 60%, the temperature is 55°C, and the extraction is performed for 3 hours. Then centrifuged (3000r / ...

Embodiment 3

[0037] Use a pump to introduce the chlorella culture solution into a membrane separation system using polysulfone (or ceramic or polyether) as the membrane material for microporous membrane filtration. The process parameters of the membrane separation are controlled as follows: the cross-flow velocity is 4 cm / s and the operating pressure is The chlorella was concentrated to 1% of the original volume at 0.08 MPa, the temperature was 40°C, and the chlorella concentrate was obtained. Add cellulase 0.3%, pectinase 0.3%, and xylanase 0.3% to the concentrate (the amount of enzyme added is the percentage of the dry matter in the chlorella concentrate), the temperature is 60℃, and the enzymatic hydrolysis is carried out for 5 hours Broken the wall to obtain a concentrated solution of broken wall chlorella. Ethanol was added to the broken chlorella concentrate to make the concentration 70%, the temperature was 50°C, and the extraction was performed for 6 hours. After centrifugation (30...

Technical field

[0001] The invention belongs to the technical field of marine biology, and particularly relates to a method for continuous extraction of chlorella functional components. Background technique

[0002] Chlorella is a pervasive single-celled green algae, belonging to the Chlorella genus Chlorella, Oocystaceae, Chlorella, Chlorellae. It is widely distributed in nature and can use light energy to autotrophically and also use organic carbon sources under heterotrophic conditions. For growth and reproduction. There are about 15 species of Chlorella in the world, plus hundreds of varieties. Chlorella is rich in nutrients and biologically active substances. Usually chlorella has a high protein content (55% to 67%), contains more sugars (15% to 20%) and lipids (5% to 10%), and contains biologically active polysaccharides, growth factors, and glycoproteins , Glycolipoprotein, Dietary Fiber, etc. It contains a complete range of amino acids in protein, including eight esse...

Examples

Embodiment 1

[0033] Use a pump to introduce the chlorella culture solution into a membrane separation system using polysulfone (or ceramic or polyether) as the membrane material for microporous membrane filtration. The control membrane separation process parameters are: cross-flow velocity 2.4cm / s, operating pressure The chlorella was concentrated to 0.5% of the original volume at a temperature of 0.02MPa and a temperature of 20°C to obtain a chlorella concentrate. Add cellulase 0.1%, pectinase 0.1%, and xylanase 0.1% to the concentrated solution (the amount of enzyme added is the percentage of dry matter in the chlorella concentrate), the temperature is 40℃, and the enzymatic hydrolysis is carried out for 1 hour Break the wall. A concentrated solution of Chlorella vulgaris was obtained. Ethanol was added to the concentrated solution of chlorella vulgaris to a concentration of 50% and a temperature of 50° C. for extraction for 2 hours. Then centrifuged (3000r / min) to obtain ethanol extract...

Embodiment 2

[0035] Use a pump to introduce the chlorella culture solution into a membrane separation system using polysulfone (or ceramic or polyether) as the membrane material for microporous membrane filtration. The process parameters of the membrane separation are controlled as follows: the cross-flow velocity is 5 cm / s and the operating pressure is The chlorella was concentrated to 0.8% of the original volume at 0.04 MPa, the temperature was 30°C, and the chlorella concentrate was obtained. Add cellulase 0.2%, pectinase 0.3%, and xylanase 0.4% to the concentrated solution (the amount of enzyme added is the percentage of dry matter in the chlorella concentrate), the temperature is 50℃, and the enzymatic hydrolysis is carried out for 2 hours Broken the wall to obtain a concentrated solution of broken wall chlorella. Add ethanol to the broken chlorella concentrate to make the concentration 60%, the temperature is 55°C, and the extraction is performed for 3 hours. Then centrifuged (3000r / ...

Embodiment 3

[0037] Use a pump to introduce the chlorella culture solution into a membrane separation system using polysulfone (or ceramic or polyether) as the membrane material for microporous membrane filtration. The process parameters of the membrane separation are controlled as follows: the cross-flow velocity is 4 cm / s and the operating pressure is The chlorella was concentrated to 1% of the original volume at 0.08 MPa, the temperature was 40°C, and the chlorella concentrate was obtained. Add cellulase 0.3%, pectinase 0.3%, and xylanase 0.3% to the concentrate (the amount of enzyme added is the percentage of the dry matter in the chlorella concentrate), the temperature is 60℃, and the enzymatic hydrolysis is carried out for 5 hours Broken the wall to obtain a concentrated solution of broken wall chlorella. Ethanol was added to the broken chlorella concentrate to make the concentration 70%, the temperature was 50°C, and the extraction was performed for 6 hours. After centrifugation (30...

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