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A kind of manufacturing method of small-molecule polypeptide of soft-shelled turtle

A technology for small molecular polypeptides and manufacturing methods, which is applied in protein food ingredients, applications, animal protein processing and other directions, can solve the problems of mixed large and small molecular weight polypeptides, poor product color and low product yield, etc. The effect of bacterial efficiency and product quality improvement

Active Publication Date: 2012-02-22
杭州中得肽生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to propose a product with advanced technology and matching, aiming at defects such as simple process, low product yield, long time consumption, low efficiency, poor product color and mixed size and molecular weight polypeptides in the existing soft-shelled turtle polypeptide production technology. A method for producing small-molecule polypeptides of soft-shelled turtles with protein content ≥ 90%, pure white, and powdery, with a yield as high as 8-9%, of which the molecular weight of polypeptides is less than 5,000 Daltons, accounting for more than 95%

Method used

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  • A kind of manufacturing method of small-molecule polypeptide of soft-shelled turtle

Examples

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Effect test

Embodiment 1

[0035] Embodiment 1: (soft-shelled turtle small molecule polypeptide preparation 1)

[0036] Follow these steps:

[0037] (1) Raw material pretreatment: Select 400kg of fresh and live soft-shelled turtle raw materials, slaughter, remove the film, remove the viscera, chop, put into an ultra-low temperature pulverizer to cool down to -170°C and pulverize, then put it into the reaction kettle, and weigh the weight of fresh and live soft-shelled turtle raw materials (The raw material weight of following each step all refers to the weight of fresh and alive soft-shelled turtle) and the ratio of water weight 1: 1 adds water 400kg; Heat up to 80 ℃ and keep warm for half an hour and then cool down to 60 ℃, set aside;

[0038] (2) Trypsin enzymolysis-boiling and heat preservation-compound enzymolysis treatment: Add 200 g of trypsin according to 0.05% of the weight of raw materials, adjust the pH value to 6.5-7.0 with 10% NaOH solution at a temperature of 55-60°C, and enzymolysis 4 hou...

Embodiment 2

[0044] Embodiment 2: (soft-shelled turtle small molecule polypeptide preparation 2)

[0045] In this example, the fresh and alive soft-shelled turtle raw material that step (1) selects is 400kg; Step (2) adds trypsin 300g by 0.075% of raw material weight, enzymolysis 2.5 hours; Adds compound protease 300g by 0.075% of raw material weight in addition, Enzymolysis for 2.5 hours; then add 24kg of perlite powder by 6% of the raw material weight; step (4) add 8kg of activated carbon by 2% of the raw material weight; Liquid 110kg; Step (6) adds 11kg of 95% edible alcohol by 10% of the concentrated solution weight, static precipitation 3 hours; Step (7) after ultrahigh temperature sterilization and spray drying, get white powdery soft-shelled turtle small molecular polypeptide finished product 34kg , and the yield is 8.5%; all the other steps and processes are the same as in Example 1.

Embodiment 3

[0046] Embodiment 3: (soft-shelled turtle small molecule polypeptide preparation 3)

[0047] In this example, the fresh and alive soft-shelled turtle raw material that step (1) selects is 400kg; Step (2) adds trypsin 400g by 0.1% of raw material weight, enzymolysis 1 hour; Adds compound protease 200g by 0.05% of raw material weight in addition, Enzymolysis for 4 hours; then add 16kg of perlite powder by 4% of the weight of the raw material; step (4) add 12kg of activated carbon by 3% of the weight of the raw material; Liquid 100kg; Step (6) adds 10kg of 95% edible alcohol by 10% of concentrated solution weight, static precipitation 4 hours; Step (7) gets white powdery soft-shelled turtle small molecule polypeptide finished product 36kg after ultra-high temperature sterilization and spray drying , and the yield was 9%; all the other steps and processes were the same as in Example 1.

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Abstract

The invention discloses a method for manufacturing soft-shelled turtle small-molecule polypeptides, and belongs to the technical field of nutrition and health product production. The method includes: pretreatment of fresh soft-shelled turtle raw materials; trypsin enzymolysis-boiling and heat preservation-combined enzyme enzymolysis treatment; high-speed separation and degreasing; activated carbon decolorization and deodorization; vacuum concentration; alcohol precipitation and impurity removal treatment; ultra-high temperature sterilization and steps such as spray drying. The method is advanced in technology, safe and hygienic, high in quality and high in efficiency, and the yield of the soft-shelled turtle polypeptide reaches 8-9%, among which small molecule polypeptides with a molecular weight below 5000 Daltons account for more than 95%, and the protein content is ≥ 90%. Pure white, powdery , in line with microbial health indicators, and is easily absorbed and utilized by the human intestinal tract and skin. The method can be popularized and applied in manufacturers of nutrition and health products and beauty and skin care products.

Description

technical field [0001] The invention relates to the technical field of production of nutrition and health care products, in particular to a method for producing small-molecule polypeptides of soft-shelled turtles using fresh and alive soft-shelled turtles. Background technique [0002] Soft-shelled turtle, also known as soft-shelled turtle and turtle, is a precious aquatic economic animal. The meat is fresh, tender and delicious, and rich in nutrition. Each kilogram of meat contains 165g of protein, 10g of fat, 16g of carbohydrates, 9g of ash, 1070mg of calcium, 1350mg of phosphorus, and iron 14mg, 6.2mg of thiamine, 3.7mg of riboflavin, 37mg of niacin, which have the effects of strengthening the body and prolonging life. In addition, soft-shelled turtles are also rich in Mg, Fe, Ca, Zn, Mn, and Se elements, which have the effects of nourishing blood, strengthening bones, improving intelligence, anti-fatigue, and anti-oxidation. Because soft-shelled turtle has high nutritio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J3/04A23J3/34
Inventor 蒋有水章珍官周学铭
Owner 杭州中得肽生物制品有限公司
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