Dual-function chitosan particle and preparation method thereof
A chitosan and bifunctional technology, which is applied in the field of magnetic resonance/fluorescence-based bifunctional chitosan particles and their preparation, can solve the problems of narrow application and cannot replace the application range of positive contrast agents, and achieve good biocompatibility. Enhanced MR imaging effect, good homogeneity and dispersion
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Embodiment 1
[0031] Mix 2mL of 25mmol / L Gd-DTPA solution with 0.5mL of 20mg / mL rhodamine B solution, and 2.5mL of chitosan (molecular weight is 25000, deacetylation degree is 86%) solution dissolved in 1% acetic acid, as water box. Take 35mL of liquid paraffin and 25mL of petroleum ether (90-120°C), add 3mL of Span 85, and mix well to form the oil phase.
[0032] Under the high-speed stirring of the homogenizer at 28000rpm, slowly add the aqueous phase solution to the oil phase for 3 minutes; then slowly add 5mL of 3mol / L NaOH solution, continue stirring at 28000rpm for 10 minutes, Centrifuge at high speed for 20 minutes, pour off the supernatant, wash and centrifuge twice with petroleum ether, acetone, ethanol and deionized water respectively, each centrifugation time is 20 minutes, and finally place it in a freeze dryer for freeze drying for 48 hour, obtain dry powdery bifunctional chitosan particles;
[0033] The components of the bifunctional chitosan particles prepared in this embod...
Embodiment 2
[0035] Mix 1mL of 25mmol / L Gd-DTPA solution with 0.5mL of 50mg / mL fluorescein sodium solution, and 1.5mL of chitosan (molecular weight is 25000, deacetylation degree is 86%) solution dissolved in 1% acetic acid, as water box. Take 25mL of liquid paraffin and 15mL of petroleum ether (90-120°C), add 1mL of Span 85, mix well, and place it in a three-necked flask as the oil phase.
[0036] Place the flask under an electric stirrer, stir at a speed of 2000rpm, slowly add the aqueous phase solution to the oil phase, and stir for 30min; then slowly add 1mL of 25% glutaraldehyde solution, and continue stirring for 60 minutes at a speed of 2000rpm, Centrifuge at 2500rpm for 20 minutes, pour off the supernatant, wash and centrifuge twice with petroleum ether, acetone, ethanol and deionized water, and finally place it in a freeze dryer for 48 hours to obtain dry powder bifunctional Chitosan particles;
[0037] The components of the bifunctional chitosan particles prepared in this embod...
Embodiment 3
[0039] Mix 0.5mL of 5mmol / L Gd-DTPA solution with 0.2mL of 50mg / mL fluorescein sodium solution, and 2.5mL of chitosan (molecular weight is 25000, deacetylation degree is 86%) solution dissolved in 1% acetic acid, as the aqueous phase. Take 25mL of liquid paraffin and 15mL of petroleum ether (90-120°C), add 1mL of Span 85, mix well, and place it in a three-necked flask as the oil phase.
[0040] Under the high-speed stirring of the homogenizer at 28000rpm, slowly add the aqueous phase solution to the oil phase for 3min; then slowly add 3mL of 3mol / L NaOH solution, continue stirring at 28000rpm for 10 minutes, Centrifuge at high speed for 20 minutes, pour off the supernatant, wash and centrifuge twice with petroleum ether, acetone, ethanol and deionized water respectively, each centrifugation time is 20 minutes, and finally place it in a freeze dryer for freeze drying for 48 hour, obtain dry powdery bifunctional chitosan particles;
[0041] The components of the bifunctional chi...
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