Method for preparing chlorogenic acid by using Jerusalem artichoke

A technology of chlorogenic acid and Jerusalem artichoke, which is applied in the field of separation and purification of biochemical products, can solve the problems of high price of chlorogenic acid products, difficulty in separation and purification, and strong infectivity, so as to improve the yield of chlorogenic acid and be economical. Benefits and social benefits, the effect of shortening the extraction time

Inactive Publication Date: 2010-06-23
NANJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the main raw materials for the preparation of chlorogenic acid are high-priced raw materials with limited resources such as honeysuckle and Eucommia leaves, resulting in high prices for chlorogenic acid products
The patent for extracting chlorogenic acid from tobacco (Chen Yuru, etc., the application of tobacco as a raw material for preparing chlorogenic acid and the method for preparing chlorogenic acid from tobacco, CN1634853), uses tobacco as a raw material for preparing chlorogenic acid, but because tobacco contains nicotine and other harmful substances, and it is not easy to separate and purify, so there are many technical problems
In the patent for extracting and isolating chlorogenic acid from Solidago (Chen Yuru et al., Method for Extracting and Separating Chlorogenic Acid from Solidago, CN101445456A), chlorogenic acid is prepared from Solidago as raw material, but Solidago has very strong infectivity , a threat to the environment

Method used

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  • Method for preparing chlorogenic acid by using Jerusalem artichoke
  • Method for preparing chlorogenic acid by using Jerusalem artichoke
  • Method for preparing chlorogenic acid by using Jerusalem artichoke

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Raw material fixation treatment: temperature 100-300°C, treatment time 5 seconds to 60 minutes.

[0038] Weigh 1.0 g of Jerusalem artichoke powder (passed through a 40 mesh sieve) and add 20 mL of distilled water with pH 4.0, leaching with ultrasonic assistance at 50°C for 40 minutes, filter, collect the filtrate, and re-extract the residue with 20 mL of distilled water with pH 4.0 40min. The filtrate was collected by filtration again. The two filtrates were combined, and finally the filtrate was adjusted to 50 mL. The extract was analyzed by HPLC, and the results were as follows: Figure 4 shown;

Embodiment 2

[0041] Add Jerusalem artichoke leaves into methanol aqueous solution with a pH of 4.0 and methanol:water 1:1.5 at a solid-to-liquid ratio of 1:20 (W / W), leaching with ultrasonic assistance at 50°C for 2 hours, and filtering the obtained solution is the crude extraction of chlorogenic acid. liquid. Concentrate the obtained extract by rotary evaporation to a volume of 5-10 mL after concentration, and collect it for later use.

[0042] Weigh 6.0 g of polyamide and pack it into a column by wet method (column size 1.5 cm×28.0 cm). The concentrated sample after extraction was separated by polyamide column, eluted with 10% ethanol, and the eluate fraction with higher content of chlorogenic acid was collected, and the ethanol was removed by rotary evaporation. After drying, the product was recrystallized from ethyl acetate. After the crystals appear, pour off the mother liquor to obtain a pure product with a chlorogenic acid content of 93%. The mother liquor can be recycled. The c...

Embodiment 3

[0044] Add the leaves of Jerusalem artichoke to a methanol aqueous solution with a pH of 4.0 and methanol:water 1:1 at a solid-to-liquid ratio of 1:25 (W / W), leaching with ultrasonic assistance at 50°C for 3 hours, and after solid-liquid separation, rotary evaporation to obtain concentrated liquid for use.

[0045] Weigh 16.0 g of macroporous resin, treat it according to the conventional pretreatment method, and then pack it into a column by wet method (column size 1.5 cm×28.0 cm). The concentrated solution after extraction was separated by column, and the adsorbed saturated resin was eluted with 40% ethanol, and the eluate components with higher chlorogenic acid content were collected, and the ethanol was removed by rotary evaporation. Finally, the product was recrystallized from ethyl acetate. After the crystals appear, the mother liquor can be poured out to obtain the refined chlorogenic acid product. Product purity can reach more than 90%. The mother liquor can be recyc...

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Abstract

The invention relates to a method for extracting chlorogenic acid from Jerusalem artichoke. The method is that, after the water of the jerusalem artichoke is removed and the enzyme is activated, the raw material is extracted by using solvent under the action of ultrasonic wave or microwave, chlorogenic acid extract liquid is obtained after solid-liquid separation, and finally pure chlorogenic acid is obtained after adsorption separation through polyamide or macroporous resin, elution and refining. The solvent can be water, organic solvent or mixed solution of organic solvent and water. Through the fixation treatment of the Jerusalem artichoke and the extraction by using the organic solvent or water under the assistance of the ultrasonic wave or the microwave, the yield of chlorogenic acid is improved and the extraction time is reduced. By using Jerusalem artichoke to extract pure chlorogenic acid, the source of raw material for preparing chlorogenic acid is widened, more valuable development and utilization of the Jerusalem artichoke are realized, and the economic benefit and the social benefit are better.

Description

technical field [0001] The invention relates to a method for extracting chlorogenic acid from Jerusalem artichoke. Polyamide or macroporous resin is used to separate and purify the extract, and combined with water or organic solvent recrystallization method to refine chlorogenic acid, which belongs to the field of separation and purification of biochemical products. Background technique [0002] Jerusalem artichoke, commonly known as Jerusalem artichoke, Latin name Helianthus tuberosus L., belongs to the Asteraceae sunflower genus annual herb, native to North America. It was introduced to Europe in the 17th century. It is also cultivated in most provinces and regions of our country. Jerusalem artichoke is mainly propagated by tubers, and the leaves are oval or oblong. The small protrusions on the surface of the blade are evenly distributed, and the back side is relatively smooth. The stem height of Jerusalem artichoke is generally about 2m, and the height can reach more t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/56
Inventor 陈育如姜中玉刘虎
Owner NANJING NORMAL UNIVERSITY
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