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Method for preparing personalized biological culture medium raw material from beer waste yeast

A technology of beer waste yeast and culture medium, which is applied in the field of preparation of beer waste yeast personalized biological culture medium raw materials, and can solve the problems of incapable scale production, low product yield, and technical limitations, etc.

Inactive Publication Date: 2010-06-23
溧阳杰康诺酵母科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the fields of medicine, food and petrochemicals such as amino acids, nisin, adenosine, penicillin, polyacrylamide and mannan peptides, due to technical limitations of yeast extract biological media, low product yields, and Can't form scale

Method used

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  • Method for preparing personalized biological culture medium raw material from beer waste yeast

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preparation example Construction

[0038] The invention provides a preparation method of a biological culture medium raw material, comprising the steps of:

[0039] (1) breaking the wall of the waste beer yeast through a nano collider under the conditions of 30-45°C and 70-100Mpa to obtain the broken yeast;

[0040] (2) Mix the broken yeast and one or more substances selected from protease or dextranase at 45-65°C for 3-24 hours to obtain yeast hydrolyzate; the protease is selected from papain or dispase; and

[0041] (3) centrifuging the yeast enzymatic hydrolyzate and performing nanofiltration with a molecular weight of 1000 to obtain a raw material for a biological culture medium.

[0042] In the enzymolysis part of step (2), it can be carried out in a variety of ways, such as mixing the broken yeast, glucanase and enzymolysis accelerator to obtain the yeast enzymolysis solution, the mixing time is 3-24 hours, relatively Preferably for 5-15 hours, the mixing temperature is 45-65°C, preferably 51-55°C; or ...

Embodiment 1

[0094] Production Method No.1 of Beer Waste Yeast Personalized Biological Medium Raw Material

[0095] 1500Kg of fresh beer yeast stock solution with a water content of 86% and a material temperature of 7.8°C was sieved twice through 100 mesh and 120 mesh, and the beer with useful value was recovered after dealcoholization by a plate and frame filter press. After beating, use a filter press machine to cycle filter and wash twice to obtain relatively high-purity yeast milk, and then use a nano-collider to physically break the wall under high pressure, pressure: 85Mpa, break the wall once, and the yeast liquid after breaking the wall Enter the reaction vessel and heat up rapidly to 95°C in 30 minutes and keep the temperature for 20 minutes, and quickly cool down to 55°C and add 10% globin peptide to carry out papain (enzyme activity 80IU, the consumption of papain is based on the dry matter of yeast clean milk , 0.2w / w%) enzymatic hydrolysis for 12 hours, the yeast enzymolysis s...

Embodiment 2

[0099] Production Method No.2 of Beer Waste Yeast Personalized Biological Medium Raw Material

[0100]1500Kg of fresh beer yeast stock solution with a water content of 86% and a material temperature of 7.8°C was sieved twice through 100 mesh and 120 mesh, and the beer with useful value was recovered after dealcoholization by a plate and frame filter press. After beating, use a filter press machine to cycle filter and wash twice to obtain relatively high-purity yeast milk, and then use a nano-collider to physically break the wall under high pressure, pressure: 85Mpa, break the wall once, and the yeast liquid after breaking the wall Enter the reaction vessel and rapidly heat up to 95°C in 30 minutes and keep the temperature for 20 minutes, and quickly cool down to 55°C and add 15% molasses yeast to carry out neutral protease (enzyme activity 80IU, the consumption of neutral protease is based on the dry matter of yeast clean milk 5w / w%) enzymatic hydrolysis for 10 hours, the yeas...

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Abstract

The invention discloses a method for preparing a personalized biological culture medium raw material from beer waste yeast, which comprises the following steps: (1) breaking the wall of beer waste yeast by using a collider at 30-45 DEG C under 70-100 MPa, thereby obtaining the wall-broken yeast; (2) mixing the wall-broken yeast, and one or a plurality of proteases or glucanases at 45-65 DEG C for3-24 hours to obtain the yeast enzymolysis solution, wherein the proteases are selected from papains or neutral proteases, and the mixing time is preferably 5-15 hours; and (3) centrifugating the yeast enzymolysis solution, and filtering through a nanofiltration membrane with the molecular weight of 1000, thereby obtaining the biological culture medium raw material. The invention also discloses the beer waste yeast personalized biological culture medium raw material obtained by using the method, and applications thereof.

Description

technical field [0001] The invention relates to a production process and a preparation method of a personalized biological medium raw material product of beer waste yeast, and industrialized comprehensive development and utilization of beer waste yeast. Background technique [0002] Saccharomyces cerevisiae is a single-celled organism, which contains about 50% of the complete protein that is easily absorbed by the body and essential amino acids that the body cannot synthesize by itself. It is rich in B vitamins, active peptides, niacin, folic acid, pantothenic acid, nucleic acid, magnesium, zinc, chromium etc., are natural green microbial protein sources. [0003] It is understood that there are more than 400 beer companies in my country and 2,000 related companies. According to the latest data from the National Bureau of Statistics, my country's beer production reached 35 million tons in 2007, maintaining the status of "the world's largest beer production and marketing cou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12P13/22C12P21/00C12R1/865
Inventor 成国祥李镇初张锁林张爱民陈东良
Owner 溧阳杰康诺酵母科技有限公司
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