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Preparation process of nanometer magnetic drug-carrying gene-carrying thermal controlled release liposome

A nano-magnetic and liposome technology, which is applied in liposome delivery, gene therapy, medical preparations of non-active ingredients, etc., can solve problems such as pigmentation, damage, and elevation, and achieve high encapsulation efficiency and dispersibility Good, good magnetic response effect

Inactive Publication Date: 2010-06-30
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the systemic administration, the plasma arsenic concentration rises rapidly and quickly diffuses into the surrounding tissues, so a small number of patients still have side effects such as gastrointestinal symptoms, peripheral neuritis, dry skin, pigmentation, and even renal impairment.

Method used

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  • Preparation process of nanometer magnetic drug-carrying gene-carrying thermal controlled release liposome
  • Preparation process of nanometer magnetic drug-carrying gene-carrying thermal controlled release liposome
  • Preparation process of nanometer magnetic drug-carrying gene-carrying thermal controlled release liposome

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Embodiment

[0027] Example A method for preparing nano-magnetic drug-loaded gene-loaded heat-controlled release liposomes as a drug for treating cancer:

[0028] A preparation method of nano-magnetic drug-loaded gene-loaded heat-controlled release liposome as a drug for treating cancer:

[0029] The first step: manganese sulfate MnSO 4 ·H 2 O, zinc sulfate ZnSO 4 ·7H 2 O, ferrous sulfate FeSO 4 ·7H 2 O is mixed in molar ratio 1: 1: 4 to obtain the composition, placed in an electric pulverizer and pulverized into powder, then weighed sodium hydroxide according to the metal ion in the composition and sodium hydroxide NaOH molar ratio of 1: 2.4 and used Dissolve in distilled water, then add aqueous sodium hydroxide solution to the powder and stir for three hours with an electric mixer, let it stand for 12 hours, then dry it in an oven at 80°C, then bake it in a muffle furnace at 400°C for 1 hour, grind the material finely after cooling into powder, soaked with 50-60°C distilled water f...

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Abstract

The invention discloses a preparation method of nanometer magnetic drug-carrying gene-carrying thermal controlled release liposome, relating to the preparation process of a drug for treating cancer, in particular to nanometer magnetic drug-carrying gene-carrying thermal controlled release liposome. Manganese sulfate, zinc sulfate and ferrisulphas are ground to powder in a certain ratio and are dissolved with a sodium hydroxide solution to obtain Mg-Zn-Fe magnetic nanoparticles by the steps of stirring, drying, roasting and the like; the obtained Mg-Zn-Fe magnetic nanoparticles are modified by a proper quantity of polyethyleneimine, and then modified Mg-Zn-Fe magnetic nanoparticles are combined with green fluorescent protein expression plasmid to obtain gene-carrying Mg-Zn-Fe magnetic nanoparticles; a proper quantity of dipalmitoyl phosphatidyl choline and cholesterol are added into an eggplant-shaped bottle to be dissolved by chloroform and absolute ether; then, the eggplant-shaped bottle is put into a rotary evaporator to be evaporated to cause the bottle bottom to form an even film; phosphate buffer containing the Mg-Zn-Fe magnetic gene-carrying nanoparticles and arsenic trioxide drug is added to be electrically stirred until the film falls off; and glycerol is added for repeated freeze thawing to obtain the nanometer magnetic drug-carrying gene-carrying thermal controlled release liposome.

Description

technical field [0001] The invention relates to a preparation process of a nano-magnetic drug-loaded gene-loaded heat-controlled release liposome as a cancer treatment drug. Background technique [0002] The invention relates to a preparation process of a new type of nano-magnetic drug-loaded gene-loaded heat-controlled release liposome, which is prepared by an improved film dispersion and high-pressure homogenization method. 2 o 3 It also encapsulates a new type of nano-thermal controlled-release liposome with manganese-zinc-ferromagnetic nanoparticles loaded with anti-tumor genes and has a suitable phase transition temperature (41°C-46°C), because the encapsulated manganese-zinc-ferromagnetic nanoparticles The variable magnetic field has the characteristics of magnetic induction temperature rise, temperature control, and constant temperature. Therefore, when the temperature rises to the phase transition temperature of liposomes, drugs and genes can be released slowly, rea...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K48/00A61K47/28A61K47/42A61K47/32A61K47/04A61K47/02A61P35/00
Inventor 张东生王丽王子妤张佳刘静
Owner SOUTHEAST UNIV
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