Detecting kit capable of simultaneously detecting 6 aquatic product pathogenic bacteria such as pseudomonas aeruginosa, etc

A technology for Pseudomonas aeruginosa and aquatic pathogenic bacteria, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve the problems of many indicators, complicated operations, and large differences in indicators.

Inactive Publication Date: 2010-09-01
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the classic physiological and biochemical identification methods, if the above six aquatic pathogenic bacteria need to be identified, they need to be identified separately, and the identification indicators are quite different, there are many indicators, and the operation

Method used

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  • Detecting kit capable of simultaneously detecting 6 aquatic product pathogenic bacteria such as pseudomonas aeruginosa, etc
  • Detecting kit capable of simultaneously detecting 6 aquatic product pathogenic bacteria such as pseudomonas aeruginosa, etc
  • Detecting kit capable of simultaneously detecting 6 aquatic product pathogenic bacteria such as pseudomonas aeruginosa, etc

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] In September 2007, bacteria were isolated from the kidneys of tilapia in a farm in Huadu, Guangzhou, and the dominant single colony was purified and cultured, and Gram staining was negative.

[0094] 1. Inoculation (operated under sterile conditions)

[0095] Use an inoculation loop to pick a small ring of bacteria into reagent B and shake well to prepare a bacterial suspension, and perform the following operations (except identification bottle 1):

[0096] Identification bottle 1:

[0097] Take out the test paper in the bottle, use the inoculation loop to pick a little bacteria and smear it on the test paper, and observe the color change of the smeared bacteria within 1 minute.

[0098] Identification vials 2-12:

[0099] Use a straw to absorb 1-2 drops of bacterial suspension into the identification bottle, put on a rubber stopper (full stopper), stand upright in the groove of the inner support, incubate at 30°C±1°C for 18-24h, observe the color of the culture mediu...

Embodiment 2

[0107] The bacteria in Table 2 were taken from the following sources, after activation culture. Carry out the identification test as follows:

[0108] Table 2

[0109]

[0110] 1. Inoculation (operated under sterile conditions)

[0111] Use an inoculation loop to pick a small ring of bacteria into reagent B and shake well to prepare a bacterial suspension, and perform the following operations (except identification bottle 1):

[0112] Identification bottle 1:

[0113] Take out the test paper in the bottle, use the inoculation loop to pick a little bacteria and smear it on the test paper, and observe the color change of the smeared bacteria within 1 minute.

[0114] Identification vials 2-12:

[0115] Use a straw to absorb 1-2 drops of bacterial suspension into the identification bottle, put on a rubber stopper (full stopper), stand upright in the groove of the inner support, incubate at 30°C±1°C for 18-24h, observe the color of the culture medium Variety.

[0116] Ide...

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PUM

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Abstract

The invention discloses a detecting kit capable of fast, simply and simultaneously detecting 6 aquatic product pathogenic bacteria such as the pseudomonas aeruginosa and the like. The kit comprises an oxidase test paper, a hydrogen sulfide culture medium, a halophilic identifying reagent, an ortho-nitrophenol beta-D-galactoside culture medium, a pectinose culture medium, a lactobiose culture medium, saccharose culture medium, a glucose culture medium, a mannose culture medium, a cellobiose culture medium, a dextrose oxidation and fermentation identifying reagent, an arginine bi-hydrolytic enzyme identifying reagent, an ornithine identifying reagent, a lysine identifying reagent, an amino acid decarboxylation identifying reagent and an indol identifying reagent. The kit has simple reagent composition and preparation method and use method, has low cost, does not need expensive apparatuses and operators with mature technology, compared with a bacterium fast automatic identifying system and a detecting technology based on the nucleic acid. The detecting kit is suitable for initially detecting the normal pathogenic bacteria of aquatic animals, is particularly suitable for the use of aquatic product popularization staff at the production line, and immediately excludes the difficulty and the anxiety for fishing peasants.

Description

Technical field: [0001] The invention relates to a detection kit for aquatic pathogens, in particular to a kit capable of simultaneously detecting Pseudomonas aeruginosa, Aeromonas hydrophila, Edwardsiella tarda, Vibrio alginolyticus, Vibrio parahaemolyticus and Vibrio vulnificus Detection kits for six aquatic pathogens. Background technique: [0002] Pseudomonas aeruginosa, Aeromonas hydrophila, Edwardsiella tarda, Vibrio alginolyticus, Vibrio parahaemolyticus, and Vibrio vulnificus are common pathogenic bacteria in aquatic animals. Monitoring the existence of these pathogenic bacteria in farmed animals and cultured water is an important measure to prevent and control related aquatic animal diseases. At present, the technologies for identifying the above-mentioned bacteria include: 1. Bacteria rapid automatic identification system, which requires expensive instruments and equipment and skilled operators; 2. Nucleic acid-based detection technology. The method requires expe...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 谭爱萍赵飞罗理王伟利姜兰邹为民陆小萏卢迈新彭华林梁爱玲
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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