Detecting kit capable of simultaneously detecting 6 aquatic product pathogenic bacteria such as pseudomonas aeruginosa, etc

Abstract

The invention discloses a detecting kit capable of fast, simply and simultaneously detecting 6 aquatic product pathogenic bacteria such as the pseudomonas aeruginosa and the like. The kit comprises an oxidase test paper, a hydrogen sulfide culture medium, a halophilic identifying reagent, an ortho-nitrophenol beta-D-galactoside culture medium, a pectinose culture medium, a lactobiose culture medium, saccharose culture medium, a glucose culture medium, a mannose culture medium, a cellobiose culture medium, a dextrose oxidation and fermentation identifying reagent, an arginine bi-hydrolytic enzyme identifying reagent, an ornithine identifying reagent, a lysine identifying reagent, an amino acid decarboxylation identifying reagent and an indol identifying reagent. The kit has simple reagent composition and preparation method and use method, has low cost, does not need expensive apparatuses and operators with mature technology, compared with a bacterium fast automatic identifying system and a detecting technology based on the nucleic acid. The detecting kit is suitable for initially detecting the normal pathogenic bacteria of aquatic animals, is particularly suitable for the use of aquatic product popularization staff at the production line, and immediately excludes the difficulty and the anxiety for fishing peasants.

Description

Technical field: [0001] The invention relates to a detection kit for aquatic pathogens, in particular to a kit capable of simultaneously detecting Pseudomonas aeruginosa, Aeromonas hydrophila, Edwardsiella tarda, Vibrio alginolyticus, Vibrio parahaemolyticus and Vibrio vulnificus Detection kits for six aquatic pathogens. Background technique: [0002] Pseudomonas aeruginosa, Aeromonas hydrophila, Edwardsiella tarda, Vibrio alginolyticus, Vibrio parahaemolyticus, and Vibrio vulnificus are common pathogenic bacteria in aquatic animals. Monitoring the existence of these pathogenic bacteria in farmed animals and cultured water is an important measure to prevent and control related aquatic animal diseases. At present, the technologies for identifying the above-mentioned bacteria include: 1. Bacteria rapid automatic identification system, which requires expensive instruments and equipment and skilled operators; 2. Nucleic acid-based detection technology. The method requires expe...

AI Technical Summary

Problems solved by technology

According to the classic physiological and biochemical identification methods, if the above six aquatic pathogenic bacteria need to be identified, they need to be identified separately, and the identification indicators are quite different, there are many indicators, and the operation is very cumbersome

At present, there are no relevant reports on the simple and simultaneous detection of six aquatic pathogenic bacteria kits including Pseudomonas aeruginosa, Aeromonas hydrophila, Edwardsiella tarda, Vibrio alginolyticus, Vibrio parahaemolyticus and Vibrio vulnificus

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