Improved method for preprocessing lignocellulose raw material

A lignocellulose and pretreatment technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, biofuels, etc., can solve the problems of high energy consumption, high pretreatment intensity, unfavorable fermentation and biorefining, etc., to achieve High yield, reduced rough cutting strength, enhanced pretreatment effect

Inactive Publication Date: 2010-09-08
EAST CHINA UNIV OF SCI & TECH
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physicochemical pretreatment methods can effectively destroy the inherent structure of lignocellulose and effectively improve the enzymatic hydrolysis efficiency of cellulose, but they all have high pretreatment intensity, high energy consumption, and some inhibitory substances generated during the pretreatment process. It is not conducive to the subsequent fermentation and biorefinery process after enzymatic hydrolysis of lignocellulose

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Roughly cut the air-dried corn stalks into 0.1-10 cm long, remove sand and stones and other sundries in the material, place the stalks in a pretreatment reactor for steam explosion pretreatment, and grind the pretreated stalks to obtain 5 Lignocellulosic fine particles of ~10mm, put the ground straw into an Erlenmeyer shaker flask with a solid content of 5% (w / w), and add cellulase Accellerase1000 TM , the enzyme amount is 7FPU / gDM, 50 ℃, 200rpm water-bath shaker enzymolysis for 24h, the conversion rate of cellulose can reach 71.06%, and the conversion rate of hemicellulose is 10.46%.

Embodiment 2

[0027] Roughly cut the air-dried corn stalks into 0.1-10 cm lengths, remove sand and stones and other sundries in the materials, place the stalks in a pretreatment reactor for steam explosion pretreatment, and grind the pretreated stalks to obtain 0.1 Lignocellulosic fine particles of ~5mm, put the ground straw into an Erlenmeyer shaker flask with a solid content of 5% (w / w), and add cellulase Accellerase1000 TM , the amount of enzyme is 7FPU / gDM, 50°C, 200rpm water-bath shaker enzymolysis for 24h, the conversion rate of cellulose can reach 71.88%, and the conversion rate of hemicellulose is 10.86%.

Embodiment 3

[0029] Roughly cut the air-dried corn stalks into 0.1-10 cm lengths, remove sand and stones and other sundries in the materials, place the stalks in a pretreatment reactor for steam explosion pretreatment, and grind the pretreated stalks to obtain 0.1 ~10mm lignocellulosic fines, put the ground straw into a bioreactor with a solid content of 30% (w / w), add cellulase Accellerase1000 TM , the enzyme amount is 15FPU / gDM, pre-enzymolysis is carried out at 50°C and 200rpm, 10% (v / v) Saccharomyces cerevisiae is inserted after 12h, simultaneous saccharification and fermentation are carried out at 37°C and 150rpm, and the fermentation is terminated after 72h. The concentration of ethanol in the fermentation broth can reach 58.68g / L, and the conversion rate of cellulose is 75.43%.

[0030] The straw treatment method involved in the present invention is not limited to steam explosion pretreatment, but can also be high temperature dilute acid pretreatment method, high temperature SO 2 O...

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Abstract

The invention relates to an improved method for preprocessing lignocellulose raw material, which comprises the following steps of: (1) roughly cutting the lignocellulose raw material to remove sand and stone impurities in coarse grains of straws; (2) preprocessing the coarse grains obtained in the step (1) in a preprocessing reactor; (3) after finishing the preprocessing, further crushing the lignocellulose material after the preprocessing into 0.1 to 10 mm lignocellulose fine grains; and (4) putting the lignocellulose fine grains obtained in the step (3) into an enzyme hydrolysis process and a fermentation process of the next step. The method has the following advantages that: while the preprocessing effect is enhanced, the energy consumption and the cost in the preprocessing process are reduced, the generation of inhibitors is reduced, and simultaneously the enzymatic hydrolysability of the lignocellulose raw material is enhanced.

Description

【Technical field】 [0001] The invention relates to the field of bioenergy, in particular to an improved pretreatment method for lignocellulosic raw materials. 【Background technique】 [0002] The structure of lignocellulosic raw materials is complex. Hemicellulose and lignin are covalently linked to form a network structure, which tightly wraps the cellulose. To directly enzymatically hydrolyze or biotransform lignocellulose, the raw materials must first be pre-processed. Treatment to reduce the degree of polymerization and crystallinity of cellulose, destroy the binding layer of lignin and hemicellulose, partially or completely remove lignin, and increase the enzymatic hydrolysis of cellulose. Therefore, pretreatment methods are crucial for the development of the biorefinery industry that uses lignocellulose as feedstock. [0003] At present, the pretreatment methods of lignocellulose mainly include physical methods, chemical methods, physicochemical methods and biological p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00C12P5/02C12P7/10C12P19/14C12R1/865
CPCY02E50/16Y02E50/343Y02E50/10Y02E50/30
Inventor 鲍杰张建楚德强毕德玺朱培芦晨阳范超
Owner EAST CHINA UNIV OF SCI & TECH
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