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Method for separating microorganisms from grease

A technology of microbial oil and separation method, which is applied in the downstream post-processing field of bioengineering, can solve the problems of difficult separation and recovery of bacteria, reduction of application value of bacteria residue, and large loss of pressing method, etc., and achieves simple and effective technology, which is conducive to large-scale industrialization The effect of less investment in production and equipment

Inactive Publication Date: 2010-09-08
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of these methods is that solid-liquid separation must be carried out before extraction. However, due to the characteristics of high cell concentration, high viscosity, and low cell density in the later stage of fermentation of oil-producing microorganisms, it is difficult to separate and recover the cells, and the cost is high.
In addition, the disadvantages of these methods are: the pressing method has a large loss, and the extraction rate is not high. Before the extraction, the obtained thalline can be dried, and the required equipment is more; the supercritical extraction method has large equipment investment and high cost. The conventional organic solvent method needs to carry out cell crushing to the microbial oil in the wet thalline after separation and purify. The crushing methods include freeze-thaw method (WO00 / 13660), enzymatic hydrolysis method (WO02 / 10423) and acid heat method (CN1923960A) etc., some of these methods are larger to the energy consumption that cell needs to be broken down, and the steps that need are loaded down with trivial details; Wherein the acid treatment method carries out the wall-breaking process to thalline, causes biomolecules such as protein in the thalline to hydrolyze or degrade, Reduced the application value of the fungus residue, poor economy

Method used

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  • Method for separating microorganisms from grease

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Experimental program
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Effect test

Embodiment 1

[0020] According to the method described in literature (Li, Y.; Zhao, Z.; Bai, F. Enzyme Microb. Technol., 2007, 41: 312-317), the oleaginous yeast Rhodosporidium toruloides AS 2.1389 grown in YEPD medium (the bacterial strain originates from the China Common Microorganism Strain Preservation Management Center) is inserted in the fermentation medium according to 10% inoculum size, 30 ℃, ventilation rate 0.8vvm, the glucose 500ml of supplementary material 1000g / L five times, obtain after cultivating 120h Fermentation broth with a dry cell weight of 92g / L (oil content 60.1%). Take 50ml of the above fermentation broth, add 50ml of chloroform, stir and extract at 20°C for 1h, settle and separate layers, remove the lower organic solvent phase, distill and dry to obtain 2.06g of oil, the oil extraction rate is 74.6%. The residue was centrifuged to obtain a solid phase, which was dried to obtain 2.31 g of bacteria residue.

Embodiment 2

[0022] According to the method described in the literature (Huang Jianzhong, Shi Qiaoqin, Zhou Xiaolan, etc. Microbiology Bulletin, 1998, (4): 187-191), the oleaginous mold Mortierella isabellina AS 3.3410 (bacterial strain derived from the Chinese Common Microorganisms Collection) was cultivated for 28h. Management center) seed solution is inserted in the fermentation medium with 10% inoculation amount, 280rpm, cultivates 70h under 25 ℃, obtains the fermentation liquid of dry cell weight 34g / L (oil content 47.2%). Take 25ml of the above fermentation broth, add 75ml of chloroform:methanol 2:1 (V / V), at 70°C, stir and extract for 1h, remove the lower organic solvent phase after centrifugation, evaporate and dry to obtain 0.37g of oil, the oil extraction rate is 92 %. The upper layer was filtered to obtain wet fungus residue, and the residue mass after drying was 0.25g.

Embodiment 3

[0024] With reference to the method described in Example 1, feed twice, obtain the oleaginous yeast Lipomyces starkeyi AS 2.1560 of dry cell weight 48g / L (fat content 55.2%) after fermenting 62h (bacterial strain originates from China Ordinary Microorganism Strain Preservation Management Center ) fermented liquid 100ml, add No. 6 solvent of 25ml, at 30 ℃, after 10min of stirring and extracting, settling and layering, take the upper solvent phase, evaporate after drying to obtain 0.72g of oil and fat, the oil extraction rate is 27%. The lower phase was centrifuged to obtain a solid phase, and 2.80 g of bacteria residue was obtained after drying.

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Abstract

The invention discloses a method for separating microorganisms from grease, which relates to a method for directly extracting grease from microorganism fermenting liquor for producing oil and co-producing microbial dreg and belongs to the technical field of downstream post treatment of biological engineering. The invention adopts the following technical scheme: mixing the microorganism fermenting liquor for producing oil and organic solvent according to certain ratio; stirring and leaching for a certain time; performing the solid-liquid separating operation; removing solvent from an organic solvent phase to obtain grease; and drying a solid to obtain the microbial dreg. The invention has the advantages of simple process, mild operating conditions, high extraction rate and yield of grease, good quality of microbial dreg, low energy consumption and favorability for scale industrial production.

Description

technical field [0001] The invention relates to a method for separating and extracting oil, which is a method for separating and extracting oil from oleaginous microorganism fermentation liquid and co-producing bacterial residue, and belongs to the technical field of downstream post-processing of bioengineering. Background technique [0002] Some microorganisms, such as yeast, mold, algae, etc., under certain conditions, convert organic matter into fatty acid glycerides and store them in the cells. The strains whose intracellular oil content can exceed the total biomass by more than 20% are called oleaginous microorganisms. Some oleaginous microbial genus fermented with sugary material as carbon source, which can accumulate more than 70% of the dry cell weight of oil (Li, Y.; Zhao, Z.; Bai, F.Enzyme Microb.Technol ., 2007, 41, 312-317). The oil obtained by fermentation is called microbial oil, also known as single-cell oil. The fatty acid composition of some microbial oils...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/00
Inventor 赵宗保赵鑫
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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