Method for preparing compound bactericide for preventing and controlling soybean root rot

A technology of soybean root rot and compound bacterial agent, which is applied in the direction of botany equipment and methods, microbe-based methods, biochemical equipment and methods, etc., can solve the problem of few reports of biocontrol bacterial agents and untargeted root pathogenic bacteria Strong and difficult to colonize with bio-control fungicides, etc., to achieve the effect of improving biological activity and control range, preventing root rot diseases, and solving colonization problems

Inactive Publication Date: 2010-09-29
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using the antagonistic effect on pathogenic fungi to screen bio-control bacteria is an effective way out for bio-control fungi, but bio-control fungi have always been difficult to colonize, have low activity, and are not very specific to root pathogens, which limits their development.
At present, the relatively good

Method used

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  • Method for preparing compound bactericide for preventing and controlling soybean root rot
  • Method for preparing compound bactericide for preventing and controlling soybean root rot
  • Method for preparing compound bactericide for preventing and controlling soybean root rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Collect healthy soybean rhizosphere soil in the field (soybean field in Fuyuan County, Jiamusi City, Heilongjiang Province), add 10g of soil to 90ml of distilled water to dilute into a bacterial suspension, use King'B solid medium, add 0.22um filter membrane to filter and sterilize the medium after sterilization Ampicillin (40ug / ml, dissolved in sterile water), chloramphenicol (13ug / ml dissolved in 95% ethanol), cycloheximide (100ug / ml dissolved in acetone), specific screening for Pseudomonas (Pseudomonas spp. );

[0022] The diluted bacterial suspension of the above soil sample was warmed in a water bath at 80°C for 10 minutes, and then Bacillus spp. was screened using beef extract peptone solid medium.

[0023] Collect the roots of soybean root rot diseased plants in the field (soybean field in Fuyuan County, Jiamusi City, Heilongjiang Province), crush the tissue in the laboratory, and use Martin's medium (after sterilization, add 2ml lactic acid to inhibit the growth...

Embodiment 2

[0029] Collect healthy soybean rhizosphere soil in the field (soybean field in Fuyuan County, Jiamusi City, Heilongjiang Province), add 10g of soil to 90ml of distilled water to dilute into a bacterial suspension, use King'B solid medium, add 0.22um filter membrane to filter and sterilize the medium after sterilization Ampicillin (40ug / ml, dissolved in sterile water), chloramphenicol (13ug / ml dissolved in 95% ethanol), cycloheximide (100ug / ml dissolved in acetone), specific screening for Pseudomonas spp ;

[0030]The diluted bacterial suspension of the above soil sample was warmed in a water bath at 80°C for 10 minutes, and then Bacillus spp. was screened using beef extract peptone solid medium.

[0031] Collect the roots of soybean root rot diseased plants in the field (soybean field in Fuyuan County, Jiamusi City, Heilongjiang Province), crush the tissue in the laboratory, and use Martin's medium (after sterilization, add 2ml lactic acid to inhibit the growth of bacteria and...

Embodiment 3

[0037] Collect healthy soybean rhizosphere soil in the field (soybean field in Fuyuan County, Jiamusi City, Heilongjiang Province), add 10g of soil to 90ml of distilled water to dilute into a bacterial suspension, use King'B solid medium, add 0.22um filter membrane to filter and sterilize the medium after sterilization Ampicillin (40ug / ml, dissolved in sterile water), chloramphenicol (13ug / ml dissolved in 95% ethanol), cycloheximide (100ug / ml dissolved in acetone), specific screening for Pseudomonas (Pseudomonas spp. );

[0038] The diluted bacterial suspension of the above soil sample was warmed in a water bath at 80°C for 10 minutes, and then Bacillus spp. was screened using beef extract peptone solid medium.

[0039] Collect the roots of soybean root rot diseased plants in the field (soybean field in Fuyuan County, Jiamusi City, Heilongjiang Province), crush the tissue in the laboratory, and use Martin's medium (after sterilization, add 2ml lactic acid to inhibit the growth...

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Abstract

The invention belongs to the technological field of agricultural production, and particularly relates to a method for preparing compound bactericide for preventing and controlling soybean root rot. The method comprises the following steps of: collecting field healthy soybean root soil, and specifically screening pseudomonas and bacillus for later use by using a King'B culture medium and a method for coating a beef extract peptone culture medium after 10 minutes of water bath at the temperature of 80 DEG C respectively; collecting roots of a field soybean root rot plant, and separating pathogenic fungi by using a martin culture medium; and re-screening bacterial strains for efficiently inhibiting the activity of the screened pathogenic fungi from the pseudomonas and the bacillus for the lateral use, fermenting the bacterial strains respectively, and then compounding the fermented bacterial strains according to a proportion to obtain the compound bactericide for preventing and controlling the soybean root rot. The screening method for preventing mixed bacteria by using soybean root growth can effectively improve the bioactivity and expand the control range; the compound bactericide is from the healthy soybean roots and most from soybean probiotics; and the colonization can be effectively performed on the soybean roots, so the utilization rate of the bactericide is enhanced.

Description

technical field [0001] The invention belongs to the field of agricultural production science and technology, and specifically relates to a method for preparing a compound bacterial agent for preventing and controlling soybean root rot. Background technique [0002] Soybean root rot is a worldwide disease with wide distribution, serious damage, various pathogenic bacteria and difficult control. Soybean root rot is mainly distributed in the northern spring soybean and southern Huanghuai summer soybean production areas in China, especially in the northeast of Heilongjiang. Soybean root rot mainly harms the soybean root system and affects the crop's absorption of water and nutrients. The occurrence of this disease can affect the root weight, root nodule weight, nitrogenase activity and lateral root number of soybean plants to varying degrees. According to investigations, the disease generally reduces production by 10-30%, and in severe cases can reach 60%-90%, or even extincti...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P3/00C12N1/20C12R1/07C12R1/38
Inventor 张惠文张晓黎阮晓东李旭李新宇苏振成
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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