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Fungicide for removing and/ or degrading polycyclic aromatic hydrocarbon and application thereof

A technology of polycyclic aromatic hydrocarbons and bacterial agents, which is applied in the biological field and can solve problems affecting the degradation rate, etc.

Inactive Publication Date: 2012-05-30
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Studies have found that a variety of microorganisms in nature can degrade polycyclic aromatic hydrocarbons, but because polycyclic aromatic hydrocarbons are stable in nature and are strongly hydrophobic substances, they are easily adsorbed by soil particles or fixed by other effects, which cut off their direct interaction with obligate microorganisms and enzymes. Therefore, accelerating the degradation of PAHs in the environment has become a new research hotspot

Method used

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  • Fungicide for removing and/ or degrading polycyclic aromatic hydrocarbon and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, Isolation and Identification of Polycyclic Aromatic Hydrocarbon Microbial Degradation Bacteria

[0029] 1. Separation

[0030] The polluted soil of the Beijing Coking Plant was inoculated into LB medium, cultured at room temperature (25-30°C) for one week, and 16 different polycyclic aromatic hydrocarbons (naphthalene NAP, acenaphthylene ANY, acenaphthylene ANE, Fluorene FLE, phenanthrene PHE, anthracene ANT, fluoranthene FLA, pyrene PYR, benzanthracene BaA, chrysene CHR, benzo[b]fluoranthene BbF, benzo[k]fluoranthene BkF, benzo[a]pyrene BaP, Indeno[1,2,3-cd]pyrene IcdP, dibenzoanthracene DahA, benzo[ghi]perylene (BghiP) were the only carbon sources, and different PAH-degrading bacteria in the soil were tested according to the plate streak method. isolated to obtain single colonies. Pick a single colony and inoculate it into a liquid medium with the corresponding polycyclic aromatic hydrocarbons as the sole carbon source. After cultivating at room temperatu...

Embodiment 2

[0041] Embodiment 2, the preparation of the bacteria agent that removes polycyclic aromatic hydrocarbons and / or degrades polycyclic aromatic hydrocarbons

[0042] 1. Preparation of active ingredients

[0043] Microbacterium (Microbacterium sp.) LU1 CGMCC No.3581, Paleobacterium sp. (Ochrobacterium sp.) LU2 CGMCC No.3582, Rhodococcus (Rhodococcus sp.) LU3 CGMCC No.3610 and Bordetella ( Bordetella sp.) LU4 CGMCC No.3611 were inoculated into four Erlenmeyer flasks containing 1L of fresh liquid medium, and after two weeks of cultivation, all the substances in the Erlenmeyer flasks were collected and called Rhodococcus sp. LU3 ferment, Microbacterium sp. LU1 ferment, Ochrobacterium sp. LU2 ferment, and Bordetella sp. LU4 ferment. Among them, the bacterial cell content in the fermentation product of Rhodococcus sp. LU3 is 1×10 6 CFU / ml, the cell content of Microbacterium sp. LU1 fermentation product is 1×10 6 CFU / ml, the cell content of Ochrobacterium sp. LU2 fermentation product...

Embodiment 3

[0048] Embodiment 3, the application of removing polycyclic aromatic hydrocarbons and / or degrading polycyclic aromatic hydrocarbons bacterial agents in soil remediation

[0049] PAH-contaminated soil was collected from Beijing Coking Plant for experiments. The soil texture is mainly sandy soil with clayey soil. The content of available nitrogen and available phosphorus is 60mg / kg and 3.5mg / kg respectively, and the content of total organic carbon is 0.6% (6000mg / kg), the total content of 16 polycyclic aromatic hydrocarbons is about 240-307mg / kg. Polycyclic aromatic hydrocarbons in soil were extracted by microwave, purified by column chromatography, and determined by GC-MS. The specific experimental steps are as follows: Weigh 3g of soil sample on a ten-thousandth balance and load it into a microwave extraction column, add 10mL of n-hexane and 10mL of acetone as extraction agents, and the extraction procedure is to raise the temperature to 100°C in 10 minutes and keep it for 10 ...

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Abstract

The invention discloses a fungicide for removing and / or degrading polycyclic aromatic hydrocarbon and application thereof. The invention provides a fungicide of which the active ingredients are (Rhodococcus sp.) LU3 CGMCC No.3610, (Microbacterium sp.) LU1 CGMCC No.3581, (Ochrobacterium sp.) LU2CGMCC NO.3582 and (Bordetella sp.) LU4 CGMCC No.3611. The experiment of the invention shows: after fourweeks, the strainaway rate of 16 types of polycyclic aromatic hydrocarbon in soil added with the fungicide is averagely improved by 30% if being compared with that of soil which is not added with thefungicide, wherein the strainaway rate of six types of low-cyclic (two / three cyclic) polycyclic aromatic hydrocarbon is averagely improved by 26%, and the stainaway rate of ten types of middle and high cyclic (more than four cycle) polycyclic aromatic hydrocarbon is averagely improved by 32%.

Description

technical field [0001] The invention relates to a bacterial agent for removing polycyclic aromatic hydrocarbons and / or degrading polycyclic aromatic hydrocarbons and its application in the biological field. Background technique [0002] Forest and grassland fires, volcanic eruptions, and endogenous synthesis of microorganisms will all produce polycyclic aromatic hydrocarbons, and unexploited coal and petroleum also contain a large amount of polycyclic aromatic hydrocarbons. With the development of industrial production, polycyclic aromatic hydrocarbons have increased greatly, and thousands of tons of polycyclic aromatic hydrocarbons are released into the earth's environmental system every year due to human activities, far exceeding the self-purification capacity of the environment. The most prominent characteristics of polycyclic aromatic hydrocarbons are strong carcinogenicity, teratogenicity and mutagenicity. Polycyclic aromatic hydrocarbons have high lipophilicity, can e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20A62D3/02B09C1/10C12R1/01A62D101/20
Inventor 卢晓霞李秀利吴淑可马杰陈超琪吴蔚廖晓勇
Owner PEKING UNIV
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