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Method for detecting ractopamine in animal-derived food by microgap array electrode-based electrochemical immunosensor

A ractopamine, array electrode technology, applied in the biological field, can solve problems such as sensitivity limitation, and achieve the effects of low background interference, low cost, and simple preparation

Inactive Publication Date: 2013-03-20
长沙市食品质量安全监督检测中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been some literature reports on the immunoassay of veterinary drug residues at home and abroad, but most of them are determined by enzyme-linked immunoassay (ELISA). Although the specificity of monoclonal antibody immunoassay technology is better, the sensitivity is still limited.

Method used

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  • Method for detecting ractopamine in animal-derived food by microgap array electrode-based electrochemical immunosensor
  • Method for detecting ractopamine in animal-derived food by microgap array electrode-based electrochemical immunosensor

Examples

Experimental program
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Embodiment Construction

[0030] Detection of ractopamine in samples using this sensor:

[0031] Sample pretreatment:

[0032] (1) Feed: The sample is crushed and passed through a 20-mesh sieve to obtain a sample. Take 2g of the sample into a 50ml centrifuge tube, add 10ml of water, mix well, put it in an ultrasonic water bath and sonicate for 30min (take out and mix once every 10min), after ultrasonic extraction, cool to room temperature, centrifuge at 2500rpm for 5min, and take the supernatant liquid for testing.

[0033] (2) Urine: Use urine to test directly. If it is turbid, please centrifuge first to get the supernatant. Urine standards must be collected in clean, dry plastic urine cups or glass containers that do not contain any preservatives. If it cannot be tested and sent for inspection in time, the urine sample standard can be stored at 2-8°C for 48 hours. Long-term storage needs to be frozen at -20°C, avoid repeated freezing and thawing.

[0034] (3) Meat: Put about 4 grams of minced po...

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Abstract

The invention relates to a microgap array electrode-based electrochemical immunosensor and a method for detecting ractopamine in animal-derived food thereof. The sensor comprises a microgap array electrode composed of an interdigital dual-electrode, wherein the widths of the comb teeth of the positive electrode and negative electrode are both 1mu m-100mu m, the electrode gap is 1mu m-100mu m, ractopamine-BSA conjugate is fixed on the surface of the microgap array electrode, and alkaline phosphatase-labeled ractopamine monoclonal antibody is added on the microgap array electrode during detection to perform competitive immunoreaction with ractopamine in a test sample and the ractopamine-BSA conjugate on the electrode. The sensor has high specificity and can realize quantitative detection and be used under 4-40 DEG C; the result can be observed in 10min, and the sensor is suitable to be used to fast detect ractopamine in the sample of animal-derived food for units or individuals.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an electrochemical immunological quantitative sensor based on a micro-gap array electrode and a method for detecting ractopamine in animal-source food by using the sensor. Background technique [0002] The common residual drugs in animal products are roughly divided into antibiotics, anthelmintics, growth promoters, antiprotozoal drugs, trypanosomiasis drugs, sedatives, hepatic adrenergic receptor blockers and others Chemical substances, etc., among which prohibited drugs include, for example: antibiotics (ciprofloxacin, enrofloxacin, etc.), stilbenes and their derivatives (diethylstilbestrol, dienestrol, etc.), β-stimulants ( ractopamine, clenbuterol, etc.), sedatives (chlorpromazine, diazepam, etc.), corticosteroids (dexamethasone, etc.), thyroid inhibitors (propylthiouracil, etc.), and sex and anabolic hormones (testosterone, estrogen, etc.) Diol, progesterone, etc.) ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/541G01N27/327
Inventor 彭瑞乾彭新凯俞汝勤沈国励蒋健晖付冠艳
Owner 长沙市食品质量安全监督检测中心