II type collagen hyaloplasm acid compound sponge bracket and use thereof

A technology of hyaluronic acid and composite sponge, applied in the field of biomedicine, can solve the problems of high production cost and high collagen concentration, and achieve the effects of maintaining mechanical properties, good performance and saving production costs.

Active Publication Date: 2013-08-07
广州市红十字会医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The collagen concentration required by the above method is too high (as described in the patent application with publication number CN101066469A, or the document Allemann, JBiomed Mater Res, 2001, 55: 13-19, the commonly used collagen solution concentration is about 0.5% is ideal), resulting in higher production costs

Method used

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  • II type collagen hyaloplasm acid compound sponge bracket and use thereof
  • II type collagen hyaloplasm acid compound sponge bracket and use thereof
  • II type collagen hyaloplasm acid compound sponge bracket and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0039] Embodiment, the preparation of type II collagen hyaluronic acid composite sponge scaffold of the present invention

[0040] 1. Preparation of high-purity type II collagen [Reference: Li Siming, Ye Chunting, etc., Preparation and detection of high-purity porcine cartilage type II collagen, Journal of Biomedical Engineering, 2001, 18(4)592-594]:

[0041] Material:

[0042] Fresh pig articular cartilage: obtained from the limb articular cartilage of freshly slaughtered pigs in the slaughterhouse

[0043] Guanidine hydrochloride: Farco 500g / bottle

[0044] Pepsin: Sigma 100g / bottle

[0045] The rest are domestic analytical reagents.

[0046] method:

[0047] Cut the hyaline cartilage from freshly slaughtered pig limb joints, cut into thin slices, degrease, mash, and homogenate, then stir with 10 times the volume of 4M (pH7.5) guanidine hydrochloride for 24 hours, and centrifuge; the sediment diameter is fully washed , Weigh the precipitated cartilage particles with a w...

experiment example 1

[0058] Experimental Example 1: Infrared Spectroscopy (FTIR) Analysis

[0059] The type II collagen hyaluronic acid composite sponge scaffold material of the present invention is compared with the type I collagen hyaluronic acid composite sponge material reported in the literature, and is measured by an infrared spectrometer (Spectrum one, Perkin-Elmer, USA).

[0060] Compared with the FTIR spectrogram, the present invention adopts the partial homogenization method to obtain the type II collagen hyaluronic acid composite sponge scaffold, and the traditional full homogenization method to obtain the type I collagen hyaluronic acid composite sponge. -1 The characteristic absorption peaks of hyaluronic acid appear in both of them, which proves that the two homogenization methods can prepare composite sponges with two characteristics of collagen and hyaluronic acid. The results are as follows: Figure 3a and Figure 3b shown.

experiment example 2

[0061] Experimental Example 2: Pore Size Detection of Type II Collagen Hyaluronic Acid Composite Sponge Scaffold

[0062] The type II collagen hyaluronic acid composite sponge support material of the present invention is compared with the type I collagen hyaluronic acid composite sponge reported in the literature and the type II collagen hyaluronic acid material prepared by the existing full homogenization method. The results are shown in Table 1

[0063] Collagen autofluorescence layered scanning was carried out on two kinds of collagen sponges by laser confocal microscope, the excitation wavelength was 488nm, the emission wavelength was 520nm, and the measurement was carried out with the limit of autofluorescence signal intensity to determine the pore size of the sponge material; using CLSM image analysis system Carry out operation and analysis, and then use SPSS13.0 software package to make statistics on the data.

[0064] The result is as Figure 2a and Figure 2b and ...

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Abstract

The invention belongs to the field of biomedicine, in particular to a bionic II type collagen hyaloplasm acid compound sponge bracket for repairing cartilage in tissue engineering. The preparation method of the II type collagen hyaloplasm acid compound sponge bracket comprises the following steps of: extracting high-purity II type collagen solution; concentrating the II type collagen solution by means of a polyethylene glycol concentration method; gradually mixing hyaloplasm acid solution with the high-purity II type collagen solution by means of a partial homogenate method; and crosslinking the concentrated II type collagen hyaloplasm acid compound sponge with EDC and NHS dual cross-linking agent to obtain the II type collagen hyaloplasm acid compound sponge bracket. The II type collagenhyaloplasm acid compound sponge bracket can be taken as a tissue engineering implant, a cell culturing bracket material or medicine controlled release and the like for the prevention and the cure of the repair of the cartilage injury.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a bionic type II collagen sponge support for cartilage repair in tissue engineering. Background technique [0002] Articular cartilage is mainly composed of a large amount of extracellular matrix and highly specific cells-chondrocytes distributed in it. There is no specific treatment for cartilage defects in clinical practice. Tissue engineered cartilage transplantation is currently the latest and most promising treatment technology for cartilage defects. One is to plant the seed cells cultured in vitro on a natural or synthetic material scaffold that can be degraded and absorbed in the body, and then transplant the composite into the defect site in animals or humans to form cartilage-like structures and functions. tissue, thereby completing the repair and reconstruction of the defect site. [0003] Tissue engineering scaffold materials actually play the role of cell growt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/56
Inventor 杨小红陈鸿辉秦胜男
Owner 广州市红十字会医院
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