Preparation method of Fe3O4 nano-magnetic powder lymphatic tracer and application thereof

A nano-magnetic powder and magnetic separation technology, applied in the preparations, pharmaceutical formulations, ferrous oxides, etc. for in vivo experiments, can solve the difficulty of lymphatic system targeting, low lymphatic uptake rate, and biocompatibility. Not ideal, etc.

Active Publication Date: 2010-11-03
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many problems still need to be studied and solved, such as the overall lymphatic uptake rate is not high; the biocompatibility of some nanomaterials in the lymphatic system is not ideal, and adverse reactions cannot be completely avoided; the basic research on the mechanism of lymphatic targeting is still insufficient However, there are still some difficulties in improving the targeting of the lymphatic system of the nano drug delivery system; the mature drug delivery route is still relatively single, and it is still limited to subcutaneous

Method used

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  • Preparation method of Fe3O4 nano-magnetic powder lymphatic tracer and application thereof
  • Preparation method of Fe3O4 nano-magnetic powder lymphatic tracer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Prepare c(Fe 3+ )=0.18mol / L, c(Fe 2+ )=0.12mol / L, after mixing as the water phase, 73.02g of cetyltrimethylammonium bromide (CTAB), 49.57g of n-butanol, and 347.01g of cyclohexane are mixed as the oil phase, and titrated with the water phase Oil phase, ultrasonic dispersion, to get W / O microemulsion. A certain amount of excess triethanolamine was added dropwise at a slow speed to control the pH value of the microemulsion. After the reaction is complete, the emulsion is broken, and the precipitate is separated with an ultra-high speed centrifuge. The obtained precipitate was washed with ethanol and distilled water three times respectively. Evaporate naturally to get nano-Fe 3 o 4 powder. The particle size of the product is 200nm.

Embodiment 2

[0028] Prepare c(Fe 3+ )=0.18mol / L, c(Fe 2+ )=0.12mol / L, after mixing as the water phase, 72.92g of cetyltrimethylammonium bromide (CTAB), 49.24g of n-butanol, and 346.01g of cyclohexane are mixed as the oil phase, and titrated with the water phase Oil phase, ultrasonic dispersion, to get W / O microemulsion. A certain amount of excess triethanolamine was added dropwise at a slow speed to control the pH value of the microemulsion. After the reaction is complete, the emulsion is broken, and the precipitate is separated with an ultra-high speed centrifuge. The obtained precipitate was successively washed three times with distilled water, hydrochloric acid and PEG mixture. Evaporate naturally to get nano-Fe 3 o 4 powder. The particle size of the product is 90nm.

Embodiment 3

[0030] 3.1 Prescription drugs

[0031] Pharmaceutical nano-Fe 3 o 4 powder 30g

[0032] Polyvinylpyrrolidone K-30 (PVP) 12g

[0033]Normal saline (0.9%) 1000ml

[0034]

[0035] Get 1000 bottles of nano-suspension injection preparation

[0036] 3.2 Preparation process

[0037] Weigh a certain amount of Fe 3 o 4 Nano-magnetic powder is dispersed in normal saline for 10mm by ultrasonic wave, then diluted with normal saline, and the pH value is adjusted to 6.9 with 10% sodium hydroxide solution with a mass fraction of 10%, and finally a medicinal Fe with a concentration of 30mg / mL is formulated with PVP as a dispersant. 3 o 4 Nanosuspension injection.

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Abstract

The invention discloses a method for preparing Fe3O4 nano-magnetic powder by a microemulsion method and preparing the nano-magnetic powder into nanosuspension which is taken as a lymphatic tracer for medicinal lymphadenectomy. The method comprises the following steps: taking ferrous chloride and ferric chloride solution as raw materials to prepare Fe<3+>and Fe<2+> aqueous solution with a certain concentration as an aqueous phase; taking cyclohexane as an oil phase, cetyl trimethyl ammonium bromide as a surfactant and n-butyl alcohol as a cosurfactant to spontaneously form a CTAB / n-butyl alcohol / cyclohexane / saline solution microemulsion system; mixing excessive triethanolamine with the microemulsion system, stirring, placing in a high-pressure kettle, and then burning at the temperature of160 DEG C for 14 hours; and cooling and carrying out magnetic separation or centrifugation on the obtained product, respectively washing with ethanol and water or hydrochloric acid and PEG, and finally carrying out magnetic separation, centrifugation or micropore filtration to obtain the Fe3O4 nano-magnetic powder. The invention has the advantages of simple process, controllable reaction conditions, cheap and available raw materials and the like.

Description

technical field [0001] The invention relates to a method for preparing nano Fe by microemulsion method 3 o 4 The method of magnetic powder, especially the use of microemulsion as a microreactor, can obtain Fe with uniform particle size, controllable, good crystallinity, and narrow particle size range. 3 o 4 Nano magnetic powder. Background technique [0002] Nano Fe 3 o 4 There are many methods for preparing magnetic powder, which can be divided into physical methods and chemical methods. Physical methods, such as mechanical pulverization, etc., are complicated in process and time-consuming and consumable. Chemical methods can be simply divided into dry synthesis and wet synthesis. Dry Synthesis of Nano Fe 3 o 4 Magnetic powder often involves oxidation-reduction reactions, requiring high equipment, numerous procedures, and time-consuming and labor-intensive processes. Therefore, the preparation of nano-Fe 3 o 4 Magnetic powder can be synthesized by wet method. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G49/08A61K49/00A61K47/02A61P35/00
Inventor 王淑君蒋艳霞支壮志葛贝尔
Owner SHENYANG PHARMA UNIVERSITY
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