Preparation process for high purity theaflavin and application thereof in preparing anti-influenza virus medicines
An anti-influenza virus and preparation process technology, applied in the field of theaflavin preparation, can solve problems such as the reduction of vaccine prevention and protection ability, the spread and spread of influenza, the inability to guarantee timely vaccination of vaccines, etc. And the effect of increasing the content and preventing deterioration
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Embodiment 1
[0105] see figure 1 , a kind of preparation technology of high-purity theaflavin of the present invention, its processing step is as follows:
[0106] a. Preparation of fermentation stock solution: add large-leaf green tea into the extractor, and the tea leaves are crushed to a fineness of 5-60 mesh, and then add organic solvents, and the ratio of green tea leaves to the added organic solvents is controlled at 1:5 -1:30 (mass ratio), the mixture was stirred and extracted at 10°C to 90°C for 10-120min, discharged, and then added with an organic solvent to extract again in the same way, and the combined extracts were placed in a vacuum of 0.085-0.054Mpa, the temperature Concentrate the extract at 56°C-80°C to a solid content of 0.5%-5% (mass ratio);
[0107] b. Add the concentrated solution of step a into the water washer for washing, and the washing reaches the quality standard of the design requirement (the quality standard of the design requirement is: catechin in the concen...
Embodiment 2
[0120] see figure 1 , the present embodiment is as the optimization of above-mentioned scheme, in the preparation process of above-mentioned step a fermentation stoste, the organic solvent that adds is ethyl acetate, and the weight ratio of green tea and the ethyl acetate that adds every time is 1: 10~1: 30; the concentrated solution extracted during the preparation of the fermentation stock solution was washed with water (step b), and the composition of catechins in the solution was controlled after washing; in the enzyme preparation process described in step c, the Freeze below 0°C, such as -10°C-0°C, and freeze for at least 2 hours, and the fineness of fresh tea leaves after crushing should be above 80 mesh; the gas introduced during the fermentation process described in step d above is pure oxygen or purified air ; The temperature should be controlled below 80°C during the concentration and transsolution process described in the above f and h steps, such as 40°C-80°C; the ...
Embodiment 3
[0122] see figure 1 , present embodiment is as the best solution of the present invention, and its steps are:
[0123] a. Preparation of fermentation stock solution: Add large-leaf green tea into the extractor, the tea leaves are crushed to a fineness of 5-60 mesh, and then add ethyl acetate, and the ratio of green tea leaves to the added ethyl acetate is controlled at 1 : 5-1: 30 (weight ratio); the mixture was stirred and extracted at 25°C to 60°C for 10 minutes to 60 minutes, and discharged, then added ethyl acetate and extracted again in the same way, and the extracts were combined. -0.054Mpa, concentrate the extract at a temperature of 56°C-80°C to a solid content of 0.5%-5% (mass ratio);
[0124] b. Water washing: the concentrated solution is added into a water washer for washing, and the washing reaches the quality standard required by the design (the quality standard required by the design is: catechins in the concentrated solution after washing are controllable sever...
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