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Method for simultaneously analyzing three kinds of oligomeric procyanidins in perfume material hawthorn tincture for tobacco

A technology of oligomeric proanthocyanidins and hawthorn is applied in the field of chemical analysis and detection, which can solve the problems of complex separation and determination, and achieve the effects of shortening analysis time, good repeatability and high recovery rate.

Active Publication Date: 2011-01-05
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the oligomeric proanthocyanidins in hawthorn tincture have various polymerization modes and degrees of polymerization, the separation and determination of each component are much more complicated

Method used

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  • Method for simultaneously analyzing three kinds of oligomeric procyanidins in perfume material hawthorn tincture for tobacco
  • Method for simultaneously analyzing three kinds of oligomeric procyanidins in perfume material hawthorn tincture for tobacco
  • Method for simultaneously analyzing three kinds of oligomeric procyanidins in perfume material hawthorn tincture for tobacco

Examples

Experimental program
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Effect test

example 1

[0026] Accurately weigh a certain amount of Procyanidins B2, (-)-Epicatechin, and Procyanidins C1 and place them in different 10mL volumetric flasks, dissolve and dilute to the mark with methanol, and shake well. The concentrations are 0.70mg / mL, 1.16mg / mL, respectively. mL and 2.20mg / mL.

[0027] Liquid chromatography analysis conditions:

[0028] Chromatographic column: waters Symmetry C18 column (4.6mm×250mm×5μm)

[0029] Flow rate: 0.8mL / min

[0030] Column temperature: 30°C

[0031] Detection wavelength: 280 nm

[0032] Injection volume: 10μL

[0033] Mobile phase A: 10% methanol containing 0.5 mL / L formic acid, mobile phase B: 30% acetonitrile containing 0.5 mL / L formic acid

[0034] Elution gradient: Time (min) 0 10 30 40 45

[0035] Mobile Phase A (V / V) 100% 100% 60% 45% 100%

[0036] Under this chromatographic condition, the three can obtain well-separated chromatograms such as figure 1 shown.

[0037] 1. Procyanidins B2, 2. (-)-Epicatechin, 3. Pr...

example 2

[0039] Take 1 mL of hawthorn tincture sample, dilute it 5 times with methanol, filter it through a 0.45 μm organic phase filter, and inject it for analysis.

[0040] Liquid chromatography analysis conditions:

[0041] Chromatographic column: waters Symmetry C18 column (4.6mm×250mm×5μm)

[0042] Flow rate: 0.8mL / min

[0043] Column temperature: 30°C

[0044] Detection wavelength: 280 nm

[0045] Injection volume: 10μL

[0046] Mobile phase A: 10% methanol containing 0.5 mL / L formic acid, mobile phase B: 30% acetonitrile containing 0.5 mL / L formic acid

[0047] Elution gradient: Time (min) 0 10 30 40 45

[0048] Mobile Phase A (V / V) 100% 100% 60% 45% 100%

[0049] Under this chromatographic condition, the chromatogram of the hawthorn tincture sample is as follows: figure 2 shown.

[0050] 1. Procyanidins B2, 2. (-)-Epicatechin, 3. Procyanidins C1

[0051] The same hawthorn tincture sample was measured 5 times in parallel, and the results showed that the met...

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Abstract

The invention discloses a method for simultaneously analyzing three kinds of oligomeric procyanidins in perfume material hawthorn tincture for tobacco, which comprises the following steps of: 1, diluting a hawthorn tincture sample with methanol, and filtering the diluted sample; and 2, performing analysis with a liquid chromatograph-diode array detector by taking a methanoic acid as a mobile-phase additive, and performing gradient elution to realize the simultaneous separation and determination of the three kinds of oligomeric procyanidins (-)-Epicatechin, Procyanidins B2 and Procyanidins C1 in the perfume material for the tobacco. Compared with the prior art, the method of the invention has the advantages of realizing the simultaneous determination of the three kinds of oligomeric procyanidins (-)-Epicatechin, Procyanidins B2 and Procyanidins C1 in the perfume material hawthorn tincture for the tobacco, and avoiding the tailing of a chromatographic peak and shortening the analysis time of the sample due to the adoption of the methanoic acid as the mobile-phase additive, along with high experimental repeatability, high recovery rate and suitability for the large-scale analysis of the samples for the tobacco in tobacco enterprises.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and detection, and in particular relates to a new method for simultaneously separating and analyzing three kinds of oligomeric proanthocyanidins (-)-Epicatechin, Procyanidins B2 and Procyanidins C1 in hawthorn tincture, a fragrance raw material for tobacco. Background technique [0002] Hawthorn, also known as "red fruit", "mountain red", Rosaceae, deciduous tree. The fruit tastes sour and slightly sweet, and is rich in vitamin C, various organic acids and flavonoids. Procyanidins belong to condensed tannins, which are mainly composed of catechin and epicatechin. They are widely distributed in the plant kingdom and also exist in some fruits and hawthorns. They are closely related to human life and health. [0003] Hawthorn tincture is obtained by extracting mature hawthorn fruit with ethanol, heating and continuously circulating reflux extraction, and recovering the solvent. For the ch...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N1/28G01N1/34
Inventor 刘绍锋郭吉兆郭军伟胡斌张晓兵谢复炜刘惠民
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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