Method for simultaneously analyzing three kinds of oligomeric procyanidins in perfume material hawthorn tincture for tobacco
A technology of oligomeric proanthocyanidins and hawthorn is applied in the field of chemical analysis and detection, which can solve the problems of complex separation and determination, and achieve the effects of shortening analysis time, good repeatability and high recovery rate.
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[0025] Example 1
[0026] Accurately weigh a certain amount of Procyanidins B2, (-)-Epicatechin and Procyanidins C1 into different 10mL volumetric flasks, dissolve and dilute to the mark with methanol, shake well, and their concentrations are 0.70mg / mL, 1.16mg / mL and 2.20mg / mL.
[0027] Liquid chromatography analysis conditions:
[0028] Column: waters Symmetry C18 column (4.6mm×250mm×5μm)
[0029] Flow rate: 0.8mL / min
[0030] Column temperature: 30℃
[0031] Detection wavelength: 280 nm
[0032] Injection volume: 10μL
[0033] Mobile phase A: 10% methanol containing 0.5 mL / L formic acid, mobile phase B: 30% acetonitrile containing 0.5 mL / L formic acid
[0034] Elution gradient: Time (min) 0 10 30 40 45
[0035] Mobile phase A (V / V) 100% 100% 60% 45% 100%
[0036] Under this chromatographic condition, the three can get well separated chromatograms such as figure 1 Shown.
[0037] 1. Procyanidins B2, 2. (-)-Epicatechin, 3. Procyanidins C1
Example Embodiment
[0038] Example 2
[0039] Take 1 mL of hawthorn tincture sample, dilute it with methanol 5 times, filter it through a 0.45μm organic phase filter, and then analyze it.
[0040] Liquid chromatography analysis conditions:
[0041] Column: waters Symmetry C18 column (4.6mm×250mm×5μm)
[0042] Flow rate: 0.8mL / min
[0043] Column temperature: 30℃
[0044] Detection wavelength: 280 nm
[0045] Injection volume: 10μL
[0046] Mobile phase A: 10% methanol containing 0.5 mL / L formic acid, mobile phase B: 30% acetonitrile containing 0.5 mL / L formic acid
[0047] Elution gradient: Time (min) 0 10 30 40 45
[0048] Mobile phase A (V / V) 100% 100% 60% 45% 100%
[0049] Under this chromatographic condition, the chromatogram of the hawthorn tincture sample is as follows figure 2 Shown.
[0050] 1. Procyanidins B2, 2. (-)-Epicatechin, 3. Procyanidins C1
[0051] The same sample of hawthorn tincture was tested in parallel for 5 times. The results showed that the method has good reproducibility and...
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