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Bacteria cellulose/gelatin/hydroxyapatite composite material and preparation method thereof

A technology of bacterial cellulose and hydroxyapatite, which is used in medical science, prosthesis and other directions, can solve problems such as poor mechanical properties, and achieve the effects of low cost, simple process, and favorable growth and reproduction.

Inactive Publication Date: 2011-01-19
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It was found that this composite material not only has a structure similar to human bone, but also has the advantages of porosity and degradability, but generally suffers from poor mechanical properties, which limits the application of this type of scaffold material in bone tissue engineering.

Method used

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  • Bacteria cellulose/gelatin/hydroxyapatite composite material and preparation method thereof
  • Bacteria cellulose/gelatin/hydroxyapatite composite material and preparation method thereof

Examples

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Embodiment 1

[0019] Add glucose, peptone, yeast powder, and disodium hydrogen phosphate to the beaker in sequence according to the mass fraction of 2.5%, 1%, 0.75%, and 1%, respectively, adjust the pH value to 4.5 with acetic acid, and take it out after half an hour of high-temperature sterilization at 115 degrees Celsius , as a growth medium for Acetobacter xylinum.

[0020] Acetobacter xylinum strains were stored in agar solid medium at 4°C. Use an iron wire to scratch lightly in the solid medium, immediately immerse in 100 ml of liquid medium, shake and cultivate at 30 degrees Celsius for 30 hours, and the shaker speed is 160 rpm, as a seed solution. Inoculate the liquid culture medium with an inoculum volume fraction of not less than 6% of the seed solution, oscillate sufficiently to make the bacterial liquid uniform, and culture at a constant temperature of 30 degrees Celsius for 5 days to obtain a bacterial cellulose gel film. Wash with 1% sodium hydroxide solution until milky white...

Embodiment 2

[0026]100 ml of 1 g / L gelatin aqueous solution was prepared, and the gel film was soaked in a pre-prepared simulated body fluid at a constant temperature for 3 days. Other experimental conditions were the same as in Example 1.

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Abstract

The invention relates to a bacteria cellulose / gelatin / hydroxyapatite composite material and a preparation method thereof. The composite material contains the raw materials of bacteria cellulose gel sheeting, gelatin, procyanidin, CaCl2 and simulated body fluid, and the mass ratio of the bacteria cellulose gel sheeting, the gelatin, the procyanidin and the CaCl2 is 100-200:1-5:4-6:6-10. The preparation method comprises the steps of: firstly crosslinking the gelatin by using the procyanidin as crosslinking agent so that the gelatin is coated on the fiber surface of the bacteria cellulose to prepare a bacteria cellulose / gelatin composite material, and the bacteria cellulose / gelatin / hydroxyapatite composite material is further prepared by adopting biomimetic mineralization method. The composite material according to the invention has excellent mechanical properties, unique three-dimensional nano-network structure and more structural and componential similarity to bionics, and meets the demand of the engineering material for cartilage tissues of human body. The composite material according to the invention has the advantages of safe and nontoxic reagents adopted, low cost and simple preparation technology.

Description

technical field [0001] The invention relates to a bacterial cellulose / gelatin / hydroxyapatite composite material and a preparation method thereof. Background technique [0002] With the development of biomedical engineering research, tissue engineering has become a hot spot in scientific research and clinical application. The study of tissue engineering scaffold materials has also attracted the attention of researchers. At present, many polymer tissue engineering scaffolds such as collagen, alginate, chitosan, and PLGA have been prepared by electrospinning and phase separation. Compared with the above-mentioned biopolymers, bacterial cellulose has special structure and physical and chemical properties. The three-dimensional nano-network structure, good mechanical properties, air permeability, and biocompatibility of bacterial cellulose make it have a very wide application potential in the field of biomedicine, and have been widely used in artificial skin, wound dressings, a...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/22A61L27/20A61L27/12C08J7/12C08J3/24C08L1/02C08L89/00C08K3/24
Inventor 万怡灶高川翟云敬
Owner TIANJIN UNIV
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