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Enzyme-linked immunoassay method for benzophenone

An enzyme-linked immunoassay and benzophenone technology, applied in the direction of measuring devices, color/spectral characteristic measurement, instruments, etc., can solve the problems of being expensive, unable to meet the requirements of fast, convenient, and accurate, and achieve high selectivity and The effect of good sensitivity, stability and repeatability, and simple sample pretreatment process

Inactive Publication Date: 2011-01-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection of benzophenone and 4-aminobenzophenone at home and abroad mainly includes high-performance liquid chromatography (HPLC), gas chromatography (GC), gas-mass spectrometry (GC-MS), liquid- Mass spectrometry (LC-MS), etc., but these methods not only require expensive instruments and equipment, professional operators, but also have relatively high requirements for test materials, and further sample pretreatment is required, which can no longer meet modern detection Requirements for fast, convenient and accurate
At present, there are no reports of enzyme-linked immunoassays for benzophenone residues at home and abroad

Method used

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  • Enzyme-linked immunoassay method for benzophenone

Examples

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Effect test

Embodiment Construction

[0055] The present invention is further illustrated by the following examples.

[0056] 1. Instrument:

[0057] TGL-40B desktop low-speed centrifuge, Shanghai Anting Scientific Instrument Factory

[0058] KFLOW pure water machine, KFLOW company

[0059] ZD-9556 horizontal shaker, Taicang Science and Education Equipment Factory

[0060] Costar 96-well 8×12 detachable microtiter plate, Shanghai Jitai Biotechnology Co., Ltd.

[0061] MuLtiska Mks Microplate Reader, Thermo Labsystems

[0062] Adjustable pipette, Thermo Labsystems

[0063] Vortex mixer, Shanghai Huxi Instrument Analysis Factory

[0064] 2. Reagents:

[0065] Horseradish peroxidase-labeled goat anti-rabbit IgG (HRP–IgG), Kangcheng Bioengineering Co., Ltd.

[0066] Tetramethylbenzidine (TMB), Huamei Bioengineering Co.

[0067] Other reagents are analytical reagents

[0068] 3. Steps

[0069] 1. Preparation of artificial antigen

[0070] Solution A: Dissolve 0.1mmol 4-aminobenzophenone in 6mL N,N-dimethylfo...

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Abstract

The invention discloses enzyme-linked immunoassay method for benzophenone, and belongs to the technical field of toxic chemical residue detection. The indirect competitive enzyme-linked immunoassay method for benzophenon in a packing material is established by immunizing by using synthesized 4-aminobenzophenone and a BSA coupling substance immunogen to obtain a polyclonal antibody, taking 4-aminobenzophenone hapten and a coupling substance of OVA as coating antigens and taking the benzophenone as a standard substance. The invention provides a quick and high-efficiency means of detecting the benzophenone residue in the packing material. Because the polyclonal antibody is adopted, the cost is relatively low and the stability and the repeatability are relatively good. The sensitivity is 0.5ppm and the linear range is between 1 and 100ppm. The ELISA has extremely high selectivity and sensitivity due to high specificity and affinity of an immune reaction.

Description

technical field [0001] The invention relates to a method for detecting benzophenone residues, more specifically, an enzyme-linked immunosorbent assay (ELISA) is used to detect benzophenone residues in packaging material paper, and belongs to the technical field of detection methods for toxic chemical residues. Background technique [0002] Benzophenone is mainly used in plastics as a light stabilizer, which can effectively protect food plastic packaging and plexiglass from deterioration due to light, and is also an intermediate of ultraviolet absorbers, organic pigments, medicines, spices, and pesticides. Due to their volatility, benzophenones can migrate from food packaging into food without functional hindrance. Studies have found that benzophenone can seriously damage the liver and kidney tissues of animals, and cause chronic cancer and reproductive toxicity. The EU Food Safety Authority has formulated the maximum migration limit of printing ink food packaging containing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N21/31G01N33/531
Inventor 胥传来林菲许宙陈莲君宋姗姗刘丽强王利兵
Owner JIANGNAN UNIV
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