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Method for comprehensively extracting cudrania tricuspidata polysaccharides and flavones

A technology of comprehensive extraction and wood, applied in the direction of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc., can solve the problems of low extraction rate, large amount of organic solvent, high cost, etc., to achieve high content of active ingredients and utilization of raw materials The effect of high rate and simple steps

Inactive Publication Date: 2011-02-16
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent application No. 200710045425.4 discloses a eucalyptus extract, its preparation and application, using 5-10 times alcoholic aqueous solution, concentrating, sequentially passing through macroporous adsorption resin column, polyamide chromatography column, gel column chromatography, and separating to obtain Total flavonoids, this method has a good separation effect, but the operation is cumbersome, the yield is low, and the cost is high, so it is not suitable for industrial production
In the "Orthogonal Test Optimizing the Extraction Process of Total Flavonoids of Z. chinensis" published by Wu Weichao et al., 12 times 60% ethanol was used to extract the total flavonoids of C.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take the Zhemu medicinal material, dry it in an oven at 55°C, crush it, and pass through a 60-mesh sieve until all the medicinal materials pass through a 60-mesh sieve. Put 500g of the sieved Zhemu powder into a multi-functional extraction tank, heat and extract with 6 times distilled water for 3 hours, and reserve the extract. Add water-containing ethanol to the dregs and heat under reflux to extract 3 times (20% ethanol, 4 times, 2 hours; 50% ethanol, 3 times, 1 hour; 80% ethanol, 3 times, 1 hour), combine the extracts, and concentrate under reduced pressure to 1600ml, first use 1L petroleum ether to ultrasonically extract, discard the extract, then extract the raffinate I with 0.8L water-saturated n-butanol, discard the extract, add 95% ethanol to the raffinate II until the precipitation is complete, and store at 4500r Centrifuge for 20 minutes at a speed of 1 / min to obtain a precipitate and a supernatant. The precipitate obtained by centrifugation was repeatedly was...

Embodiment 2

[0024] Take the Zhemu medicinal material, dry it in an oven at 55°C, crush it until all the medicinal materials pass through an 80-mesh sieve, put 1500g of the sieved Zhemu powder into a multifunctional extraction tank, heat and extract it with 5 times distilled water for 4 hours, and keep the extract. Add water-containing ethanol to the dregs, heat and reflux to extract twice (20% ethanol, 4 times, 3 hours; 60% ethanol, 3 times, 2 hours), combine the extracts, concentrate under reduced pressure to 2200ml, and first use 3.0L petroleum ether to sonicate Extraction, discard the extract, extract the raffinate I with 1.5L water-saturated n-butanol, discard the extract, add 95% ethanol to the raffinate II until the precipitation is complete, and centrifuge at 6000r / min for 30 minutes to obtain pellet and supernatant. The precipitate obtained by centrifugation was repeatedly washed with acetone, the solvent was evaporated to dryness at low temperature, and freeze-dried to obtain the...

Embodiment 3

[0027] Take the Zhemu medicinal material, dry it in an oven at 55°C, crush it until all the medicinal materials pass through a 40-mesh sieve, put 5000g of the sieved Zhemu powder into a multi-functional extraction tank, and first heat and extract it with 5 times distilled water for 2 times, each time for 4 hours , retain the extract, add water-containing ethanol to the dregs and heat under reflux to extract 4 times (20% ethanol, 3 times, 3 hours, 1 time; 50% ethanol, 3 times, 2 hours, 2 times; 80% ethanol, 2 times, 1 hour, 1 time), combine the extracts, concentrate under reduced pressure to 10L, first use 12.0L petroleum ether for ultrasonic extraction, discard the extract, then extract the raffinate I with 5.5L water-saturated n-butanol, discard the extract , Add 95% ethanol to the raffinate II until the precipitation is complete, and centrifuge at 3000r / min for 35 minutes to obtain the precipitation and supernatant. The precipitate obtained by centrifugation is repeatedly wa...

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Abstract

The invention discloses a method for comprehensively extracting cudrania tricuspidata polysaccharides and flavones, relating to a natural plant extraction technology. The method comprises the following technical scheme of: drying a cudrania tricuspidata medicinal material in a drying oven at 55 DEG C, pulverizing, screening through a 40-mesh to 80-mesh sieve, adding different concentrations of ethanol solutions, extracting through gradient refluxing, concentrating the extraction liquid, extracting by using petroleum ether and water saturated normal butanol in sequence, adding 95% ethanol into the extraction liquid until completely precipitating, and centrifugally separating to obtain the centrifugal precipitate and supernatant; repeatedly washing the precipitate with acetone, and freeze-drying to obtain cudrania tricuspidata polysaccharides; and leading the supernatant to pass through a chromatographic column, collecting a fraction containing flavones, concentrating under reduced pressure, and drying to obtain the cudrania tricuspidata flavones. The method has high yield and can be used for preparing medicines or heath care products with anti-tumor effect.

Description

Technical field: [0001] The invention belongs to the technical field of natural plant extraction, and relates to a method for comprehensively extracting eucalyptus polysaccharides and flavonoids. Background technique: [0002] Cudrania tricuspidata Carr. [Cudrania tricuspidata (Carr.) Bur.] is the wood of the Moraceae plant Maclura tricuspidata Carr. [Cudrania tricuspidata (Carr.) Bur.]. The compounds isolated and identified from Cudrania tricuspidata are mainly xanthones and flavonoids, polysaccharides, in addition to alkaloids, Lignans, coumarins and other ingredients. [0003] Polysaccharides are a kind of natural polymer compounds, which are polymers connected by monosaccharides. They are widely found in animals, plants and microorganisms. They have clear anti-tumor and immune-enhancing effects. Immune function, humoral immune function, macrophage function, and NK cell (natural killer cells) function have different activation effects. And because polysaccharides have n...

Claims

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Application Information

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IPC IPC(8): A61P35/00C08B37/00A61P37/04A61K36/60
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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