Method for producing salt-free compound amino acid powder from combination of sesame seed meal and soybean meal

A compound amino acid, combined production technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as single amino acid composition, and achieve the effect of simple preparation method, mild conditions and good product quality.

Inactive Publication Date: 2013-01-23
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the disadvantages of a large amount of salt in the production of amino acids in the prior art and a single amino acid composition, the present invention uses sesame meal and soybean meal as raw materials, undergoes raw material pretreatment, simultaneous enzymatic fermentation, filtration, concentration, and spray drying and other processes to obtain salt-free amino acid powder

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Sesame meal dry powder 0.5kg, soybean meal 0.5kg, mix, grind to 40 mesh, add 3 kg of water, heat to 90°C, heat-resistant a-amylase 150 units, keep for 90min, cool to 60°C Add 100 units of glucoamylase per gram of mixture and keep it for 90 minutes. Cool to 50°C and add 200 units of cellulase per gram of mixture, keep warm for 2 hours, then cool to 40°C, add 2000 units of compound protease per gram of mixture. Based on the weight of the mixture, 0.08% of high-temperature resistant Saccharomyces cerevisiae high-activity dry yeast is added to carry out simultaneous enzymatic hydrolysis and fermentation. The composite protease described therein is composed of four kinds of proteases, neutral protease, acid protease, alkaline protease and papain, and the mixing weight ratio thereof is 1:1:1:1.

[0024] (2) Enzymolysis and fermentation for 4 days at the same time, and filtration. Obtained filtrate 3.2kg, filter residue 1.6kg. Add 20 g of activated carbon, heat to boil,...

Embodiment 2

[0027] (1) Sesame meal dry powder 0.2kg, soybean meal 0.8kg, mixed, crushed to 60 mesh, added 10kg of water, heated to 70°C, 70 units of high temperature-resistant a-amylase, kept for 30min, cooled to 60°C according to Add 100 units of glucoamylase per gram of mixture and keep it for 30 minutes. Cool to 50°C and add 100 units of cellulase per gram of the mixture, keep warm for 2 hours, then cool to 28°C, and add 1000 units of compound protease per gram of the mixture. Based on the weight of the mixture, 0.02% of high-temperature resistant Saccharomyces cerevisiae high-activity dry yeast is added to carry out simultaneous enzymatic hydrolysis and fermentation.

[0028] (2) Enzymolysis and fermentation for 7 days at the same time, and filtration. 9.3 kg of filtrate and 1.5 kg of filter residue were obtained. Add 30g of activated carbon, heat to boil, heat and stir to decolorize for 2h, filter, filter the cake after washing and filtering with 1kg of water, and concentrate the c...

Embodiment 3

[0031](1) Sesame meal dry powder 0.3kg, soybean meal 0.7kg, mix, grind to 50 mesh, add 5kg of water, heat to 80°C, heat-resistant a-amylase 120 units, keep for 90min, cool to 60°C according to Add 100 units of glucoamylase per gram of mixture and keep it for 60 minutes. Cool to 50°C and add 150 units of cellulase per gram of the mixture, keep warm for 2 hours, then cool to 35°C, and add 2000 units of compound protease per gram of the mixture. Based on the weight of the mixture, 0.06% of high-temperature resistant Saccharomyces cerevisiae high-activity dry yeast is added to carry out simultaneous enzymatic hydrolysis and fermentation.

[0032] (2) Enzymolysis and fermentation for 6 days at the same time, and filtration. Obtained filtrate 4.5kg, filter residue 1.3kg. Add 10g of activated carbon, heat to boil, heat and stir to decolorize for 4 hours, filter, and concentrate the filtrate at 0.04~0.08MPa until it is further concentrated to 27 degrees Baume, and the volume percent...

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PUM

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Abstract

The invention discloses a method for producing salt-free compound amino acid powder from the combination of sesame seed meal and soybean meal as raw materials, which relates to a production method of compound amino acid powder. The method comprises the following steps of: (1) crushing the mixture of the sesame seed meal and the soybean meal, adding water into the powder, adding amylase, alpha-amylase, cellulase and compound protease into the powder respectively, and adding high-temperature resistant brewing high-activity dried yeast into the mixture to perform enzymolysis and fermentation at the same time; (2) after 4 to 7 days of heat-preservation enzymolysis and fermentation, performing filtration for the first time, and adding active carbon of which the weight is 1 to 4 percent of thatof filtrate into the filtrate to perform decolorization for 2 to 4 hours in a boiling state; and (3) performing spray drying on concentrated solution. Compared with an acid hydrolysis method and an alkaline hydrolysis method, the method causes no environmental pollution, and the produced leftovers can also be processed into feeds; the amino acid composition of the product is more reasonable, which can greatly improve the amino acid score of the product; and the soybean meal has a beany flavor, and the sesame seed meal has a sesame flavor, so the sensory score of the amino acid product obtained by compounding the soybean meal and the sesame seed meal is improved.

Description

technical field [0001] The invention relates to a method for producing salt-free compound amino acid powder, in particular to a method for jointly producing salt-free compound amino acid powder by using sesame meal and soybean meal as raw materials. Background technique [0002] Sesame meal is a by-product of sesame seed oil extraction, with an average crude protein content of 42.5%~47.9%, which is a high-quality resource for the preparation of amino acids. On the one hand, compared with silkworm chrysalis and blood meal, the use of sesame meal can greatly reduce the production cost of amino acid powder; on the other hand, sesame meal has high quality and rich content of essential amino acids, such as methionine, cystine and tryptophan The content of methionine and arginine is higher than that of bean cake, cottonseed cake and rapeseed cake, and the content of methionine alone is 40% higher than that of bean cake. The content of other amino acids such as threonine and leucin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00C12P7/08C12R1/865
Inventor 王振斌马海乐任晓锋孙亚钊马晓珂
Owner JIANGSU UNIV
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