Domestication method for microcoleus vaginatus and application thereof

The invention relates to a technology for the preparation method of microcystis, which is applied to the domestication of microcystis and its application field, which can solve the problems of lack of soil nutrients, large material and financial resources, high treatment costs, and the like, so as to solve the problems of soil salinization and increase in fertility. , the effect of increasing the biomass of microalgae

Inactive Publication Date: 2013-09-18
北京芳能科技有限公司
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

) Among the existing main methods for controlling saline-alkali land, the water washing and salt discharge adopted by the physical method are likely to cause the loss of soil nutrients, and require large material and financial resources, while the fertilization or acid-base balance adopted by the chemical method is likely to cause soil compaction and Excessive governance costs
Although physical and chemical methods can temporarily change the salinity of the soil, they cannot essentially change the soil fertility of the original saline-alkali land.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Domestication method for microcoleus vaginatus and application thereof
  • Domestication method for microcoleus vaginatus and application thereof
  • Domestication method for microcoleus vaginatus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: process the saline-alkali area along the coast of Yingkou, Liaoning

[0058] step 1:

[0059] 1. prepare the FN33 substratum of the present invention's culture Microcoleum:

[0060] Ammonium nitrate 3.0g / L Magnesium sulfate 0.04g / L

[0061] Dipotassium hydrogen phosphate 0.05g / L Calcium chloride 0.015g / L

[0062] Disodium EDTA 0.1g / L Ferric Chloride 0.002g / L

[0063] Peptone 1.0g / L Sodium Chloride 2.0g / L

[0064] Trace elements 0.5ml / L

[0065] Wherein the trace element preparation method is:

[0066] Boric acid 286mg Copper sulfate 5mg

[0067] Manganese Chloride 115mg Ammonium Molybdate 3.7mg

[0068] Zinc sulfate 12mg Add distilled water to make up to 1L

[0069] The culture conditions are: temperature 25°C, light 22,000 lux, pH: 8.0, after 7 days of culture, the dry cell weight of Nannotheca can reach up to 1.8g / L, such as figure 2 shown.

[0070] 2. The formula of BG-11 medium is as follows:

[0071] Sodium nitrate 1.0g / L Magnesium sulfate...

Embodiment 2

[0101] Treatment of saline-alkali areas along the coast of Yingkou, Liaoning

[0102] Step 1: Prepare the culture medium (basic medium) for cultivating Microcoleum:

[0103] Ammonium nitrate 1.0g / L Magnesium sulfate 0.05g / L

[0104] Dipotassium hydrogen phosphate 0.02g / L Calcium chloride 0.03g / L

[0105] Disodium EDTA 0.2g / L Ferric Chloride 0.003g / L

[0106] Peptone 1.2g / L Sodium Chloride 3.0g / L

[0107] Trace elements 1.0ml / L

[0108] The culture conditions are: temperature 24°C, light 20000 lux, pH: 8.5, growth cycle 8 days.

[0109] Step 2: Adaptive domestication of Microsheath according to the use of saline-alkali land:

[0110] (1) Salinity tolerance acclimatization:

[0111] Ammonium nitrate 1.0g / L Magnesium sulfate 0.05g / L

[0112] Dipotassium hydrogen phosphate 0.02g / L Calcium chloride 0.03g / L

[0113] Disodium EDTA 0.2g / L Ferric Chloride 0.003g / L

[0114] Peptone 1.2g / L Trace elements 1.0ml / L

[0115] Sodium chloride gradually increased from 6g / L to 11g / L

...

Embodiment 3

[0132] Treatment of saline-alkali areas along the coast of Yingkou, Liaoning

[0133] Step 1: Prepare the medium for cultivating Microcoleum:

[0134] Ammonium nitrate 1.4g / L Magnesium sulfate 0.02g / L

[0135] Dipotassium hydrogen phosphate 0.03g / L Calcium chloride 0.04g / L

[0136] Disodium EDTA 0.05g / L Ferric Chloride 0.005g / L

[0137] Peptone 0.8g / L Sodium Chloride 3.0g / L

[0138] Trace elements 1.5ml / L

[0139] The culture conditions are: temperature 26°C, light 28000 lux, pH: 8.5, growth cycle 8 days.

[0140] Step 2: Adaptive domestication of Microsheath according to the use of saline-alkali land:

[0141] (1). Salinity tolerance acclimatization:

[0142] Ammonium nitrate 1.4g / L Magnesium sulfate 0.02g / L

[0143] Dipotassium hydrogen phosphate 0.03g / L Calcium chloride 0.04g / L

[0144] Disodium EDTA 0.05g / L Ferric Chloride 0.005g / L

[0145] Peptone 0.8g / L Trace elements 1.5ml

[0146] Sodium chloride gradually increased from 6 to 12g / L

[0147] After being accli...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a domestication method for microcoleus vaginatus and application thereof. The method comprises the following steps of: preparing a special culture medium, performing domestications such as salt tolerance domestication, nitrogen-phosphorus content reducing domestication, adaptability to water to be treated and the like on the screened algae seeds, introducing the algae seeds into a saline-alkali land after gradual amplification culture, propagating a great amount of microcoleus vaginatus by using photosynthesis, wherein the microcoleus vaginatus absorbs and fixes nitrogen and phosphorus elements in the atmosphere and the water source; and draining the microcoleus vaginatus culture water after propagation, and depositing the microcoleus vaginatus in the soil, wherein the microcoleus vaginatus contains a large amount of elements such as nitrogen, phosphorus and the like. After one-time treatment of the method, the nitrogen in the soil is increased by 19 to 22 percent, the phosphorus is increased by 13 to 15 percent, and the full salt quantity is reduced by 25 to 30 percent; and the method avoids nutrient element loss caused by a washing method, avoids hardening of the soil and secondary pollution of the water source and the environment due to the application of fertilizers, can gradually enhance the soil fertility by multiple times of treatment, can accelerate soil recovery, and finally fulfills the purpose of improving the ecological environment.

Description

technical field [0001] The invention discloses a method for improving saline-alkali land and increasing soil fertility by using nitrogen-fixing blue-green algae, and in particular relates to a method for treating saline-alkali land by using Microcoleum algae. Background technique [0002] Saline-alkali lands are widely distributed around the world, covering more than 30 countries on six continents. It is mainly distributed in India, Pakistan, Russia, South Africa and other countries, with a total area of ​​about 956 million hectares. Among them, India and Pakistan have 8.09 million hectares, and Russia has 7.5 million hectares. The area of ​​saline-alkali land in the western United States accounts for about half of the irrigated area, and the output of saline-alkali is reduced by more than 25% every year. Land salinization is an important part of land desertification, which seriously threatens the local agricultural and animal husbandry production, the prosperity and devel...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C09K17/00C12R1/89C09K109/00
Inventor 单东杰李涛
Owner 北京芳能科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products