Inactivation method of pathogenic microorganisms of chitosan

Abstract

An inactivation method of the pathogenic microorganisms of chitosan belongs to the technical field of the biological material processing. The method comprises the following steps: performing primary inactivation to the pathogenic microorganisms of chitosan with NaOH solution, then drying chitosan, introducing nitrogen, sealing, and performing 10-50kGy of gamma-ray irradiation at -20 to 30 DEG C to inactivate possible pathogenic microorganisms in chitosan and ensure the safety of chitosan used as the biological material. Compared with the existing inactivation method of the pathogenic microorganisms, by using the method of the invention, the pathogenic microorganisms such as bacteria and viruses can be effectively killed, the quality indexes of chitosan such as deacetylation degree and crystallization degree can not change and the reduction of the molecular weight is obviously lower than that of other inactivation methods. Therefore, the quality and function of the biological material can be maintained while the pathogenic microorganisms brought by the possible pollution can be effectively killed.

Description

Technical field [0001] The invention involves chitosan, especially the pathogenic microorganisms of a chitosan, which belongs to the field of biomagonic material processing technology. Background technique [0002] The named polycal chemical name is Jug (1,4) -2-amino-2-deoxida-β-D-polysaccharis, which is mainly extracted from the abolished animal shell and fungal cell walls widely existing.It is a natural amino polysaccharide.Because of its good biocompatibility and biodegradation, shell polysaccharides are widely used in biomaterials, medicine, food and other fields.However, chitosan is derived from the animal and may be contaminated from various pathogenic microorganisms from viruses to bacteria. At the same time, chitosan may also be affected by factors such as instruments and utensils, reagents, and production environments in processing and storage.Pollution of pathogenic microorganisms such as viruses.If these pollutants are spread to people, they will cause serious health ...

AI Technical Summary

Problems solved by technology

[0004] But these methods have limitations on the inactivation of chitosan pathogenic microorganisms: the S / D method is only effective for enveloped viruses; the heating method is less effective for heat-resistant viruses , and the experiment found that heating will cause the degradation of chitosan products: chitosan turns from off-white to brown after dry heat sterilization at 170°C for 2 hours; chitosan turns brownish yellow after dry heat sterilization at 120°C for 4 hours, The molecular weight decreased to 27.6% of the initial value; chitosan changed from off-white to yellow after 121°C damp heat sterilization, and the molecular weight decreased to 56.8% of the initial value

γ-ray is an effective method to inactivate pathogenic microorganisms in chitosan, but a higher radiation dose is required to completely inactivate pathogens in chitosan, and the degradation of chitosan is more significant at high doses, which is not quality has some influence

NaOH inactivation of pathogenic microorganisms in chitosan is limited by the two-phase system