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Activating agent for enhancing degradability of oil degrading bacteria as well as application and use method thereof

A technology of activator and degrading bacteria, which is applied in chemical instruments and methods, preservation of microorganisms, water/sludge/sewage treatment, etc., can solve problems affecting system treatment efficiency, BOD increase, scum accumulation, etc., to achieve efficient metabolism, Reduce processing costs and improve processing effects

Inactive Publication Date: 2012-06-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the oily sewage treatment system, the lack of trace elements will lead to an increase of volatile fatty acids, resulting in a decrease in pH, an increase in BOD, and accumulation of scum, which will eventually affect the treatment efficiency of the entire system

Method used

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  • Activating agent for enhancing degradability of oil degrading bacteria as well as application and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: the preparation method of the activator that strengthens grease degradation ability

[0015] The activator for enhancing the degradation ability of the oil-degrading bacteria includes a microbial activator, a vitamin solution and a trace element solution with a volume ratio of 1:1:1. The preparation methods of the three solutions are as follows: Take Tween801.0mL, KH 2 PO 4 0.02g, MgSO 4 ·7H 2 00.01g, K 2 HPO 4 Put 0.15g, 0.5g of sucrose, and 1000mL of distilled water in a 1L narrow-mouth bottle, stir with a glass rod to dissolve it, and obtain a microbial stimulator; take 0.5g of vitamin A, 10.5g of vitamin B, 0.5g of vitamin C and 1000mL of distilled water In the bottle, stir with a glass rod to dissolve it to obtain a vitamin solution; take ZnSO 4 ·7H 2 O 0.22g, CoCl 2 ·6H 2 O 0.08g, MnCl 2 4H 2 O 0.54g, CuSO 4 ·5H 2 O 0.08g, NaMoO 4 ·H 2 O 0.08g, NiCl 2 ·6H 2 O 0.08g, Na 2 SeO 3 0.08g, HBO 4 0.005g and 1000mL of distilled water were...

Embodiment 2

[0016] Embodiment 2: the preparation method of the activator that strengthens grease degradation ability

[0017] The activator for enhancing the degradation ability of the oil-degrading bacteria includes a microbial activator, a vitamin solution and a trace element solution with a volume ratio of 1:1:1. The preparation methods of the three solutions are as follows: Take Tween8010.0mL, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 00.5g, K 2 HPO 4 Put 4.5g, 20g of sucrose, and 1000mL of distilled water in a 1L narrow-mouth bottle, and stir it with a glass rod to dissolve it to obtain a microbial stimulator; Stir with a rod to dissolve it, and obtain a vitamin solution; take ZnSO 4 ·7H 2 O 0.66g, CoCl 2 ·6H 2 O 0.40g, MnCl 2 4H 2 O1.44g, CuSO 4 ·5H 2 O 0.80g, NaMoO 4 ·H 2 O 0.73g, NiCl 2 ·6H 2 O 0.61g, Na 2 SeO 3 0.66g, HBO 4 0.02g and 1000mL of distilled water were placed in a 1L narrow-mouth bottle, stirred with a glass rod to dissolve, and a trace element solution was obt...

Embodiment 3

[0018] Embodiment 3: The activator is added to the upflow anaerobic sludge bed reactor (UASB reactor)

[0019] An upflow anaerobic sludge bed reactor for treating oily sewage in the laboratory, with an effective volume of 18L. According to the dosage of 0.03% of the volume of sewage, take 5.4mL of microbial activator and add it to the reactor. Dosing once every 8 days thereafter, the dosage is the same as above.

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Abstract

The invention discloses an activating agent for enhancing degradability of oil degrading bacteria as well as application and a use method thereof. The activating agent comprises a microorganism excitant, a vitamin solution and a trace element solution in the proportion of 1:1:1, wherein the three solutions are independently preserved before usage, the microorganism excitant contains 1.0-10.0mL / L of Tween80, 0.02-1.0g / L of KH2PO4, 0.01-0.5g / L of MgSO4.7H20, 0.15-4.5g / L of K2HPO4 and 0.5-20.0g / L of cane sugar, the vitamin solution contains 0.5-1.0g / L of vitamin A, 0.5-10.5g / L of vitamin B1, and0.5-10.0g / L of vitamin C, and the trace element solution contains 0.05-0.15g / L of zinc, 0.02-0.10g / L of cobalt,, 0.15-0.40g / L of manganese, 0.02-0.20g / L of copper, 0.04-0.35g / L of molybdenum, 0.02-0.15g / L of nickel, 0.04-0.30g / L of selenium, and 0.0007-0.003g / L of boron. When the activating agent is used, the microorganism activating agent accounting for 0.03% of the volume of oil sewage is respectively taken and directly added into a sewage treatment system once 7-14 days. The activating agent is used for oil sewage treatment and can enhance the degradability of oil degrading microorganisms.

Description

technical field [0001] The invention relates to an activator for enhancing the degradation ability of oil-degrading bacteria and its application and application method. The activator is composed of biological stimulants, vitamins and trace elements, which can promote the growth of microorganisms, enhance metabolic activity, improve system stability and reduce processing costs. Background technique [0002] Sewage from slaughterhouses, dairy processing plants, restaurants, etc. contains a large amount of non-biodegradable oils and fats. If high-concentration oily sewage is discharged into the surrounding water without treatment, it will form an oil film on the water surface, hinder oxygen transmission, cause water hypoxia, and threaten the survival of aquatic animals and plants. In anaerobic biological treatment, grease will accumulate on the sludge surface, hinder the transmission of soluble pollutants, and reduce the degradation efficiency of microorganisms on pollutants; ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04C02F3/34
Inventor 陈英旭梁志伟刘国防
Owner ZHEJIANG UNIV
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