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Method for producing sorbierite by using metal ions to preprocess cells

A metal ion and production method technology, applied in the field of sorbitol, can solve the problems of high energy consumption, environmental pollution, complex production process, etc., and achieve the effects of a feasible production process, avoiding a cell treatment process, and a simple treatment method

Inactive Publication Date: 2011-03-30
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most methods for the production of sorbitol and gluconate from Z. mobilis involve osmotic pretreatment of Z. mobilis cells, which increases yield but also increases cost
Adding chemical reagents such as toluene and CTAB brings great pressure to the subsequent separation work, and these chemical reagents are easy to cause environmental pollution, which is not practical for industrial applications
Freeze-drying Zymomonas mobilis or repeated freeze-thaw cycles at -20°C resulted in increased sorbitol production with almost no ethanol production; Freeze-drying or repeated freezing and thawing requires a lot of energy, and prolongs the production cycle, making the production process more complex and costly
In addition, the production of sorbitol under high sugar concentration (650g / L glucose + fructose) can improve the conversion rate of sorbitol, but the raw material cannot reach such a sugar concentration in the actual production process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The catalytic reaction is carried out in a fermenter, the total reaction volume is 500ml, and the mother liquor of glucose and fructose is 600g / L. Measure 125ml of mother liquor of glucose and fructose in the fermenter, then measure 100mL of deionized water, add 1g of heptahydrate Zinc sulfate, raise the temperature in the fermenter to 39°C, then suspend 30.53g (wet weight) cells with 150mL deionized water, add to the fermenter, the final reaction system contains 157.110g / L glucose, 158.855g / L fructose and 2g / L zinc sulfate heptahydrate, OD 600 : 68.000. The amount of final sorbitol is 161.055g / L, productive rate is 1.014 (g / g), conversion rate is 1.003; Wherein contrast (do not add metal ion) glucose 153.743g / L, fructose 153.685g / L, final sorbitol The amount is 138.255g / L, the yield is 0.899 (g / g), and the conversion is 0.856.

Embodiment 2

[0025] The catalytic reaction is carried out in a fermenter, the total reaction volume is 500ml, and the mother liquor of glucose and fructose is 600g / L. Measure 125ml of mother liquor of glucose and fructose in the fermenter, then measure 100mL of deionized water, add 5g of chlorinated Calcium, the temperature in the fermenter was raised to 39°C, and then 30.43g (wet weight) cells were suspended with 150mL deionized water and added to the fermenter. The final reaction system contained 158.394g / L glucose, 158.170g / L fructose and 10g / L calcium chloride, OD 600 : 60.600. The amount of final sorbitol is 160.103g / L, productive rate is 1.012 (g / g), conversion rate is 1.001; Wherein contrast (do not add metal ion) glucose 153.743g / L, fructose 153.685g / L, final sorbitol The amount is 138.255g / L, the yield is 0.899 (g / g), and the conversion is 0.856.

Embodiment 3

[0027] The catalytic reaction is carried out in a fermenter, the total reaction volume is 500ml, and the mother liquor of glucose and fructose is 600g / L. Measure 125ml of mother liquor of glucose and fructose in the fermenter, then measure 100mL of deionized water, and add 1g of pentahydrate Copper sulfate, raise the temperature in the fermenter to 39°C, then suspend 30.31g (wet weight) cells with 150mL deionized water, add to the fermenter, the final reaction system contains 160.123g / L glucose, 159.097g / L fructose and 2g / L copper sulfate pentahydrate, OD 600 : 62.000. The amount of final sorbitol is 161.002g / L, productive rate is 1.012 (g / g), conversion rate is 1.001; Wherein contrast (do not add metal ion) glucose 153.743g / L, fructose 153.685g / L, final sorbitol The amount is 138.255g / L, the yield is 0.899 (g / g), and the conversion is 0.856.

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Abstract

The invention relates to a method for producing sorbierite by using metal ions to process cells, comprising the following steps of adding metal ions into a system for producing sorbierite by using glucose and fructose as substrates and the zymomonas mobilis which is Gram-negative bacteria to process the cells so as to inhibit the activity of various kinds of enzyme for ethanol metabolism in the zymomonas mobilis, effectively inhibit ethanol generation, and completely convert the glucose and the fructose into sorbierite and gluconic acid. The invention has the advantages that the glucose and the fructose can be completely converted into the sorbierite and the gluconic acid by adding few metal ions into a reaction system; the processing method is simple and avoids the traditional complicated cell processing process; in addition, the products of zinc gluconate and calcium gluconate which are generated by adding some metal ions, such as Zn2+, Ca2+, and the like are objective products so that the burden of a subsequent separating and purifying process is not increased, and the production process of the sorbierite is more feasible.

Description

【Technical field】 [0001] The invention relates to the technical field of sorbitol, in particular to a method for producing sorbitol by pretreating cells with metal ions. 【Background technique】 [0002] Sorbitol is one of the 12 key intermediates with the most development potential evaluated by the National Renewable Energy Laboratory (NREL) and the Pacific Northwest National Laboratory (PNNL). Using sorbitol as a biorefinery intermediate, a variety of important materials and chemicals that can replace petrochemicals can be produced. The main derivatives include isosorbide, propylene glycol, polysorbate fatty acid ester ( sorbitan fatty acid esters, SFAE), lactic acid, glycerin, polysaccharides (branchedpolysaccharides), etc., all of which have important industrial value. The current industrial production technology of sorbitol is a 50% D-glucose syrup catalytic hydrogenation process, which consumes a lot of energy to produce sorbitol, and the recovery cost is high and cause...

Claims

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Application Information

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IPC IPC(8): C12P7/18C12R1/01
Inventor 鲍杰刘长俊董宏伟
Owner EAST CHINA UNIV OF SCI & TECH
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