Application of?mixed bdellovibrio swimmer and bdelloplast bacterial liquid in sea cucumber seedlings cultivation

The technology of leech vibrio and mixture is applied in the application field of leech vibrio swimmer and leech plast mixture in sea cucumber breeding, and can solve the problem that there is no research on the mixed preparation of leech vibrio and leech plast. Reports and other issues to achieve the effect of improving the immunity of seedlings, strong vitality and good effect

Inactive Publication Date: 2011-04-20
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, there is no research report on the use of the mixed preparation of Bdellovibrio swimmers and Bleuplasma in the process of raising sea cucumber seedlings

Method used

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  • Application of?mixed bdellovibrio swimmer and bdelloplast bacterial liquid in sea cucumber seedlings cultivation
  • Application of?mixed bdellovibrio swimmer and bdelloplast bacterial liquid in sea cucumber seedlings cultivation
  • Application of?mixed bdellovibrio swimmer and bdelloplast bacterial liquid in sea cucumber seedlings cultivation

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The preparation of embodiment 1 Bdellovibrio mixed preparation

[0026] The Bdellovibrio brudoloid bacterium liquid is preferably prepared by the following method: inoculate 0.5mL 10 6 cfu / mL Aeromonas hydrophila (Aeromonas hydrophila, purchased from Guangdong Provincial Institute of Microbiology Culture Collection Center, number GIM1.172) in 100mL nutrient broth (10g peptone, 3g beef extract, 5g sodium chloride, 1000mL distilled water, pH 7.4) liquid medium, cultured at 28°C for 20 hours, and the culture medium was centrifuged at 6000rpm for 20 minutes, and then precipitated with 5mL DNB (dilute nutrient broth) liquid medium (0.8g nutrient broth, 0.5g casein acid hydrolyzate, yeast extract Extract 0.1g, dissolve in 1000mL distilled water, pH value is 7.4) suspend, then add to 100mL DNB, insert 1mL 1×10 3 pfu / mL Bdellovibrio BDFM05, cultured at 28°C for 48h, centrifuged at 7000rpm for 20min, removed the supernatant containing Bdellovibrio swimmers, and suspended the pr...

Embodiment 2

[0029] Application experiment of embodiment 2 Bdellovibrio mixed preparation in raising sea cucumber seedlings

[0030] Implementation location: a sea cucumber farm in Shandong, seedling species: imitation sea cucumber, also known as gray cucumber

[0031] 1. Personally participate in the harvesting of natural sea areas. Harvesting time: when the bottom water temperature reaches 15-16°C, ginseng will be harvested intensively 5 days before spawning. Select individuals with a body length of more than 20cm, a body weight of more than 250g, a gonadal index of more than 10%, and no damage as relatives.

[0032] 2. Ginseng storage

[0033] Stocking density 15 / m 3 , the water temperature for storage is 16-18°C, and the storage time is 5 days, and it is carried out in a storage pond. The water in the reservoir is finely filtered. Add Bdellovibrio swimming body and Bdellovibrio mixed bacterial solution in water, make the Bdellovibrio concentration in the cultivation pool reach 10 ...

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Abstract

The invention discloses the application of mixed formulations of bdellovibrio swimmer and bdelloplast in sea cucumber seedlings cultivation which is realized by adding mixed bdellovibrio swimmer and bdelloplast bacterial liquid into water and feeding stuffs. The application comprises specifically the following parts: seedlings cultivation facilities and water quality conditions, parent sea cucumber fishing, parent sea cucumber cultivation, spawning, hatching and seedling cultivation, seedling collection and sea cucumber fingerling cultivation. The key technique is that 10-10<7> pfu/ml mixed bdellovibrio formulations, by concentration, is added into water, and/or feeding stuffs is soaked in 10-10<7> pfu/ml mixed bdellovibrio formulations for about 30 minutes. By application of this invention, the average survival rate of sea cucumber seedlings can reach more than 50% and water quality can be effectively improved. Relevant experiments demonstrate that water can be kept for at most 20 days; thus, this invention can save cost, labour, water and energy, is low-carbon and environmentally-friendly, and greatly boost economical interests with high seedling survival rate and good seedling quality.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a bacterium, in particular to the application of a bacterial solution of a mixture of bdellovibrio swimmers and bdeloids in sea cucumber seedling cultivation. Background technique [0002] Sea cucumber (Apostichopus japonicus), belonging to the class Holothurioidea, feeds on seabed algae and plankton. Sea cucumbers are covered with thorns and are widely distributed in oceans all over the world. Sea cucumber is as famous as ginseng, bird's nest and shark's fin, and is one of the eight treasures in the world. Sea cucumber is not only a precious food, but also a valuable medicinal material. Modern research shows that sea cucumber has the functions of improving memory, delaying gonad aging, preventing arteriosclerosis, diabetes and anti-tumor. [0003] In recent years, with the continuous improvement of the living standards of urban and rural residents and the enhance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A23K1/18A23K1/16C12N1/20C12R1/01A23K10/18A23K50/80
CPCY02A40/81
Inventor 蔡俊鹏孙丽滢
Owner SOUTH CHINA UNIV OF TECH
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