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Method for preparing L-ornithine by utilizing enzyme method

An enzymatic preparation, ornithine technology, applied in the field of L-ornithine preparation, can solve the problems of complex process, difficult separation, high cost, and achieve the effects of short cycle, strong specificity and low energy consumption

Inactive Publication Date: 2011-05-04
XINYI HANLING BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The synthesis method is the preparation method of ornithine in the early research. The process is complicated and the cost is high. The product D-ornithine and L-ornithine are both, which are not easy to separate, so they have been eliminated.

Method used

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  • Method for preparing L-ornithine by utilizing enzyme method
  • Method for preparing L-ornithine by utilizing enzyme method
  • Method for preparing L-ornithine by utilizing enzyme method

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Experimental program
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Effect test

Embodiment 1

[0019] Embodiment 1: A method for enzymatically preparing L-ornithine, mainly comprising the following steps:

[0020] ①. Feeding: Add 4.5kgL-arginine hydrochloride and 2.7kg manganese sulfate into a jacketed reactor with a capacity of 100L, then add 30kg of deionized water, and stir evenly, then measure the pH value 6, add 30% sodium hydroxide solution to adjust the pH value of the solution in the reactor to 9.5, then add 2.64 kg (5.8 million units) of L-arginase;

[0021] ②. Reaction: Control the steam and circulating water volume of the reactor to keep the temperature in the reactor at 36°C, keep the temperature for 20 hours, and obtain the reaction liquid after the reaction;

[0022] ③. pH adjustment: adjust the pH of the reaction solution to 7 with 37% hydrochloric acid to obtain a neutral solution;

[0023] 4. Concentration: Carry out vacuum distillation to described neutral liquid, distill off about 22kg of water, obtain concentrated liquid;

[0024] ⑤. Precipitation:...

Embodiment 2

[0030] Embodiment 2: present embodiment is the trial production that certain factory does according to the present invention, and concrete method is with embodiment 1, and concrete data sees table 1 below:

[0031] Table 1

[0032]

[0033] The solvent prepared by the enzymatic method of the present invention is deionized water, and the reaction materials include L-arginine hydrochloride, L-arginase, manganese sulfate and concentrated hydrochloric acid, and the raw materials used for extracting L-arginase include bovine liver , Tris-HCl buffer and a small amount of manganese chloride are non-polluting raw materials. The deionized water used in the reaction process and the anhydrous ethanol used in the post-treatment process can be recycled and recycled, which can greatly save costs. The final reaction The waste liquid contains enzymes, which can be removed by heating to make it solid. A small amount of manganese sulfate solution and urea are free of pollutants and can be d...

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Abstract

The invention discloses a method for preparing L-ornithine by utilizing an enzyme method. Reaction materials mainly comprise L-arginine hydrochloride, L-arginase, manganese sulfate and concentrated hydrochloric acid; raw materials for extracting the L-arginase mainly comprise beef liver, Tris-HCl buffer solution and a small amount of manganese chloride; and the reaction materials are finally prepared into the L-ornithine through the steps of adding the materials, reacting, regulating the pH value, concentrating, precipitating, filtering and the like. The invention has the characteristics of simple process, short period, low energy consumption, strong specificity, high yield, no pollution and the like.

Description

technical field [0001] The invention relates to a method for preparing L-ornithine, in particular to a method for preparing L-ornithine by enzymatic method. Background technique [0002] L-ornithine exists in various tissues and cells of organisms, has biological activity, and plays an important role in the metabolism of organisms. It is an intermediate product of urea production, a prerequisite substance for the metabolism of arginine, citrulline and other amino acids, mainly participates in the ornithine cycle of urea production, and has a detoxification effect on the accumulated ammonia in the body. Human liver cells are of great significance. In the modern pharmaceutical industry, L-ornithine is not only used to prepare various compound amino acid infusions such as protecting the liver, protecting the liver, treating liver diseases, anti-fatigue, and anti-weakness, but also often used together with arginine to prepare hair for recovery from fatigue. Make a drink. In r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/10C12N9/78
Inventor 陈士安
Owner XINYI HANLING BIO ENG
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