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Grass carp injection vaccine synergist and preparation method thereof

A technology of a synergist and a vaccine, which is applied to the field of injection vaccine synergist and its preparation, can solve the problems of unstable adjuvant effect and large side effects in animals, and achieves simple and easy operation, low cost and synergistic effect of the preparation method. obvious effect

Inactive Publication Date: 2011-05-18
GUANGDONG HAID GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of the prior art such as unstable adjuvant effect of immune injections and large side effects on animals, and provide a vaccine synergist for grass carp injection

Method used

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  • Grass carp injection vaccine synergist and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Preparation of synergist

[0023] Medium formula: 20g glucose + 15g peptone + 5g sodium chloride + 0.5g beef extract + 20g agar, dissolve in 1L distilled water and then autoclave. After culturing Bacillus subtilis in the above liquid medium for 12 hours, it was washed 3 times with 0.01 M PBS, pH 7.2 phosphate buffered saline, and then adjusted to 3 × 10 with PBS. 10 CFU / mL, stored at 4°C. Before injection, weigh the yeast β-1,3 glucan and galactomannan oligosaccharide according to the volume of the bacterial solution, so that the final concentrations in the synergist are 0.2 g / mL and 0.1 g / mL, respectively. Add to the vaccine at 1:50 and use it now.

Embodiment 2

[0024] Example 2: Preparation of synergist

[0025] Medium formula: 20g glucose + 15g peptone + 5g sodium chloride + 0.5g beef extract + 20g agar, dissolve in 1L distilled water and then autoclave. After culturing Bacillus subtilis in the above liquid medium for 12 hours, it was washed 3 times with 0.01 M PBS, pH 7.2 phosphate buffered saline, and then adjusted to 3 × 10 with PBS. 10 CFU / mL, stored at 4°C. Before injection, weigh the yeast β-1,3 glucan and galactomannan oligosaccharide according to the volume of the bacterial solution, so that the final concentrations in the synergist are 0.2 g / mL and 0.1 g / mL, respectively. Add to the vaccine at 1:100, and use it now.

[0026]

Embodiment 3

[0027] Embodiment 3: the synergistic effect of the synergist of the present invention on grass carp attenuated vaccine

[0028] The synergists in Examples 1 and 2 were selected to synergize the attenuated grass carp vaccine. Grass carp attenuated vaccine is attenuated grass carp freeze-dried cell vaccine produced by Pearl River Fisheries Research Institute. Select healthy grass carp species with a body weight of 50±5g and a total of 600 tails. They were stocked in three cages of 3m × 2m × 2m at a water temperature of 28±2°C. After being kept for 7 days to confirm that they were disease-free, immunization injection was carried out. Effect group, normal vaccine group, control group (PBS), 0.2 mL / tail was injected at the base of the dorsal fin. After 28 days of immunization, 30 experimental fish in the cage were randomly selected and injected with 0.3 mL of 1:10 virus tissue plasma for challenge, continuous After 14 days of observation, the mortality rate was counted, and the su...

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Abstract

The invention discloses a grass carp injection vaccine synergist. One milliliter of the synergist comprises the following effective components: 0.1-0.2g of yeast beta-1,3glucan, 0.05-0.1g of galacto-mannan-oligoscessharides and 3*108-3*1010CFU of bacillus subtilis. The invention also discloses a preparation method of the grass carp injection vaccine synergist, which comprises the steps of: adding 1-2g of yeast beta-1,3glucan and 0.5-1g of galacto-mannan-oligoscessharides in 10 ml of bacillus subtilis liquid and mixing uniformly. The invention also discloses the application of the grass carp injection vaccine synergist to the vaccination of grass crap. Compared with the prior art, the grass carp injection vaccine synergist has less components, is prepared with a simple method, has obvious synergy on grass carp vaccines, and can improve the protection effect of vaccines on grass carp and prolong immunity protection period.

Description

technical field [0001] The invention relates to an injection vaccine synergist for grass carp and a preparation method thereof. Background technique [0002] At present, the problem of grass carp disease is still one of the important factors that determine the success of breeding. Although traditional chemical drug control can play a certain role, it has also caused problems such as drug resistance of pathogens, accelerated pathogen mutation frequency, and imbalance in the breeding environment, which seriously threatens human health and is not conducive to the sustainable development of aquaculture. Immune prevention and control is to increase the level of specific antibodies of aquatic animals to pathogens through immunization, thereby reducing the occurrence of diseases. Immune prevention and treatment has no toxic and side effects, is green and environmentally friendly, and can fundamentally improve the body's resistance. It is an inevitable trend in the prevention and ...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K36/064A23K1/16A61P37/04A61K31/715A61K31/716A61K35/74A23K10/18A23K20/163A23K50/80A61K35/742
Inventor 余露军
Owner GUANGDONG HAID GROUP
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