Device and method for biological culture of cell or tissue engineering

A technology for tissue engineering and biological culture, which is applied in the field of reactors for combined culture of cells and biological materials, and can solve the problem of tissue engineering constructs that cannot be constructed with tissue engineering cell scaffold complexes, have little effect on improving the oxygen content in the culture, and Problems such as poor mass transfer, to achieve the effects of convenient monitoring, easy liquid and gas supply, and stable ring swing force

Active Publication Date: 2011-06-08
韩春茂
View PDF5 Cites 29 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the wave bioreactor uses the up and down reciprocating motion provided by the shaking table to enhance the mass transfer. This method has insufficient lateral mass transfer and the cells at both ends may collide with the walls of the culture bag during large wave movements, resulting in Cell damage; while the torrent bioreactor uses the horizontal rotation movement provided by the shaker to strengthen the horizontal mass transfer, but the culture medium at the bottom is still horizontally rotating, and there are still defects of poor vertical mass transfer; it is especially important Unfortunately, these two bioreactors can only be used for the expansion of ordinary animal cells. There is no case for tissue engineering seed cells, nor can they be used f

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Device and method for biological culture of cell or tissue engineering
  • Device and method for biological culture of cell or tissue engineering
  • Device and method for biological culture of cell or tissue engineering

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Such as figure 1 The shown device of the present invention for cell or tissue engineering organism culture includes a culture container, a driving device and an online liquid supply, gas supply and monitoring system. in:

[0037] 1. Culture container

[0038] A cell culture container 10 with a U-shaped, W-shaped or V-shaped bottom surface is designed on the pendulum tray, which can be made of glass, plastic, stainless steel, etc., preferably polypropylene plastic. When using the glass culture vessel 10 in combination with microcarrier technology for cell culture, the inner wall of the vessel needs to be siliconized in advance. There is a top cover 25 on the top of the two kinds of culture containers 10, which is sealed with the container below, but it can be disassembled. There is an opening in the central part of the top cover, which respectively accommodates the liquid feeding pipe 8, the liquid outlet pipe 9, the gas outlet pipe 28, and the dissolved oxygen electro...

Embodiment 2

[0049] Suspension culture of cells: Suspension culture of cells can be anchorage-independent cells, or a combination of anchorage-dependent cells and microcarrier culture techniques. The treated and sterilized U-shaped and W-shaped bottom cell culture containers or other culture containers and cushions are placed on the ring pendulum tray, and the height of the support pulley is adjusted to make the tray form a certain angle with the horizontal plane. 5-30 is recommended. °, after adjusting the concentration of the cells to be cultured, add the cells into the culture container through the liquid feeding tube on the upper cover of the culture container, then add a certain amount of medium, and then connect the inlet, outlet, monitoring electrodes and gas in and out The pipeline; start the stepping motor to make the tray rotate; if the microcarrier culture is used, the speed of the ring pendulum is slow at the beginning, 15-60 rpm is recommended, and the speed of the ring pendulu...

Embodiment 3

[0051]Cultivation of tissue engineering scaffold cell complexes: It is recommended to choose a culture container with a V-shaped bottom surface. When performing gas-liquid two-phase and gas-liquid interface culture, the angle of the rotating surface can be further increased to 15-45°, and the V-shaped The bottom side wall of one side of the culture container is approximately horizontal, and the culture solution can be added to be 1 to 5 cm higher than the tissue engineering construct, and the height of the liquid level can be controlled by adjusting the height of the liquid outlet tube, and the tray is swung in a slow circular motion, preferably 0.2 cm. ~12 rev / min, so that the culture solution flows slowly along the bottom wall of the culture container, so that the tissue engineering constructs on the bottom wall can be completely submerged or gas-liquid two-phase culture, adjust the size of the container, the amount of culture solution added And the ring swing speed of the tr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a bioreactor and method, particularly a reactor and method for seed cell culture or cell/biological material compound culture in cytobiology, tissue engineering and medicine. The method for biological culture of cell or tissue engineering comprises the following steps: putting biological culture raw materials of cell or tissue engineering into a culture vessel with a U-shaped bottom surface, W-shaped bottom surface or V-shaped bottom surface; and driving the culture vessel to annularly swing by using an annular swing power system, wherein the culture vessel annularly swings by using the center as the pivot, and each point on the same circumference sequentially swings up and down. The invention also discloses a device for implementing the method. Under the action of the power provided by the external annular swing, the invention can reduce the cell damage and provide a uniform culture environment under ideal mass transfer conditions.

Description

technical field [0001] The invention relates to a bioreactor and a method, in particular to a reactor and a method for performing seed cell culture or compound culture of cells and biological materials in cell biology, tissue engineering and medicine. Background technique [0002] Tissue engineering is a science that applies the principles and methods of engineering and life sciences to construct biological substitutes to restore, maintain or enhance the function of damaged organs or tissues. The construction of this biological substitute requires biological materials, seed cells and a suitable culture environment, and the bioreactor that can link the three together. [0003] In the field of tissue engineering, most of the seed cells used to construct tissue engineered tissues or organs are anchorage-dependent cells, and the source of seed cells is often primary cultured cells. From the perspective of safety, these cells should not be genetically modified. Transformation an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M3/00C12M1/38C12M1/34C12M1/04C12N5/00C12N5/02
CPCC12M23/50C12M27/16
Inventor 韩春茂胡信雷王新刚于玮洁孙华凤有传刚胡行
Owner 韩春茂
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap