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Chromium-removing rhodobacterium and application thereof

A red bacteria and chromium removal technology, applied in the field of microorganisms, can solve the problems of harsh culture conditions, less industrial application, and low reduction of Cr, and achieve the effects of low cost, avoiding pollution, and reducing the concentration of total chromium

Inactive Publication Date: 2012-09-26
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently isolated bacteria all have a low concentration of tolerance and reduction of Cr(VI), and the culture medium and culture conditions required for bacterial growth are relatively harsh, and most of them are limited to the treatment of acidic or neutral chromium-containing wastewater. , the reduction rate is slow, and there are few industrial applications

Method used

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  • Chromium-removing rhodobacterium and application thereof
  • Chromium-removing rhodobacterium and application thereof
  • Chromium-removing rhodobacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, dechroming rhodobacter CGMCC No.3512 ( Pannonibacter phragmitetus LSSE-09) strain screening

[0038] Soil samples were collected from the Cr(VI)-contaminated soil next to the chromium salt pile. Suspend 5g of soil sample in 25ml of normal saline, put it in a shaker and mix it for 30-60min, let it stand still, absorb the supernatant, and put it in 100ml of selective medium (the supernatant can be directly added to the selection medium for the water sample) culture medium). The composition of the selective medium is: deionized water 1000ml, tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, and K 2 Cr 2 o 7 (Cr(VI) concentration is 300mg / l) as the chromium source, adjust pH 8.0-10.0, place on a shaker at 150rpm, culture at 37°C for three days, dilute the bacterial suspension, and spread it on a selective solid medium plate. The isolated single colonies were respectively inoculated in selective medium with a Cr(VI) concentration of 300 mg / l,...

Embodiment 2

[0045] Embodiment 2, dechroming rhodobacter CGMCC No.3512 ( Pannonibacter phragmitetus LSSE-09) Obtaining resting bacteria

[0046] Pick the Rhodobacter dechrome bacteria CGMCC No.3512 (Pannonibacter phragmitetus LSSE-09) cultured on the nutrient slant, and add it to 40 ml of the basal medium. The ingredients of the culture medium: 1000ml of deionized water, 10g / L of tryptone, 5g / L of yeast extract, and 10gL of sodium chloride. Adjust pH to 7.0, temperature 37°C, 150rpm shaker to activate culture for 12h, then add it to 500ml basal medium according to 1% inoculum amount. After 12 hours, the bacterial cells were obtained by centrifugation, and then washed with 0.9% normal saline, and repeated three times to obtain the bacterial cells.

Embodiment 3

[0047] Embodiment 3, dechroming rhodobacter CGMCC No.3512 ( Pannonibacter phragmitetus LSSE-09) Acquisition of active dried bacteria

[0048] Pick the Rhodobacter dechrome bacteria CGMCC No.3512 (Pannonibacter phragmitetus LSSE-09) cultured on the nutrient slant, and add it to 40 ml of the basal medium. The ingredients of the culture medium: 1000ml of deionized water, 10g / L of tryptone, 5g / L of yeast extract, and 10gL of sodium chloride. Adjust pH to 7.0, temperature 37°C, 150rpm shaker to activate culture for 12h, then add it to 500ml basal medium according to 1% inoculum amount. After 12 hours, the bacterial cells were obtained by centrifugation, and then washed with 0.9% normal saline, and repeated three times to obtain the bacterial cells. Freeze and vacuum dry to obtain active stem cells.

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Abstract

The invention relates to the microbial field, in particular to a chromium-removing rhodobacterium and an application thereof. The CGMCC No. of the chromium-removing rhodobacterium is 3512. The chromium-removing bacterium (CGMCC No.3512) provided by the invention is screened and separated from the nature; whether the rhodobacterium is in a grown state or a static state, the bacterium can be used to reduce the Cr (VI) in wastewater to Cr (III) selectively, rapidly and effectively and generate Cr(OH)3 precipitate, thus the total chromium concentration of wastewater can be reduced and the environmental pollution can be avoided. The new strain of the chromium-removing rhodobacterium of the invention can grow in the alkaline environment and be used to treat the wastewater containing Cr (VI); and the strain has the incomparable advantage of high reduction rate compared with the common bacterial reduction method. Meanwhile, the development of Cr (VI)-containing wastewater treatment technology adopting the biological method can be promoted and the pollution problems of chromium-containing wastewater in the Chinese chemical industries such as chromium salt industry and electroplating industry can be solved.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a dechroming rhodobacter and application thereof. Background technique [0002] The chromium salt industry is an important branch of the inorganic salt industry. Its main products include sodium dichromate, chromic anhydride, potassium dichromate, and chromium oxide. Chromium compounds are widely found in the wastewater discharged from chromium salt production, textile dyeing, dye production, tanning, electroplating and other industries. From 1991 to 1995, relevant departments found that Cr and Pb are relatively common heavy metal pollutants in the monitoring of domestic urban rivers, seven major water systems and lakes and reservoirs. Heavy metals in water enter the human body through direct drinking water, eating vegetables and grains irrigated by sewage, etc., threatening people's health. The common chromium compounds in industrial wastewater are Cr(III) and Cr(VI) in two valen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/22
Inventor 刘会洲徐林
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI