Detection genetic chip and detection kit for infectious diarrhea

Abstract

The invention provides a genetic chip and a detection kit related to major pathogenic microorganisms causing infectious diarrhea of human beings, which are mainly specific to 13 types of bacteria including lapactic bacillus coli / shigella, salmonella, comma bacillus, vibrio parahemolyticus, aeromona hydrophila, plesiomonas, virio hollisae, vibrio fluvialis, vibrio mimicus, Wauter's strains, vibrio damsela and vibrio furnissii. The genetic chip comprises a solid-phase carrier and an oligonucleotide probe fixed on the solid-phase carrier, wherein the oligonucleotide probe comprises DNA (Deoxyribonucleic Acid) fragments selected from genes including a gyrB gene, an ITS gene, a dnaJ gene, a toxR gene and the like with remarkable biological evolution advantages or complementary DNA fragments. By adopting the genetic chip and the detection kit provided by the invention, major pathogenic microorganisms causing infectious diarrhea of human beings can be detected. The genetic chip and the detection kit have the characteristics of easiness and convenience for operation, high flux, high accuracy, high repeatability and the like, and can be applied to clinical detection of inspection departments in hospitals.

Description

technical field [0001] The invention belongs to the technical field of gene chip applications, and relates to a gene chip and a detection kit containing the chip, in particular to a gene chip and a detection kit for the main pathogenic microorganisms causing human infectious diarrhea. Background technique [0002] Infectious diarrhea is one of the infectious diseases with high incidence and widespread prevalence in the world. It poses serious health hazards to humans, especially children, and is an important public health problem in developing countries. According to WHO estimates, tens of millions of people suffer from the disease every day in the world, with 3 to 5 billion cases of diarrhea every year, and 5 to 10 million deaths due to severe diarrhea, with an average of 25,000 deaths per day, especially children. It is prominent that adults in developed countries have diarrhea at least 1 or 2 times a year, and the incidence in developing countries is higher. Diarrhea kil...

AI Technical Summary

Problems solved by technology

In the field of pathogenic bacteria detection, many detection technologies based on PCR method have been established at home and abroad, but because PCR technology can only target one or several pathogenic bacteria, and there are many kinds of pathogenic bacteria in water, this requires At the same time, most common pathogenic bacteria are detected. This limitation of PCR greatly increases the workload of detection, and it is not suitable for rapid detection of a large number of samples in the fields of water quality detection and epidemiological analysis of aquatic pathogens.

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