Gene engineering bacterium capable of both tolerating high-concentration As (Arsenic) (III) and oxidizing As (III) and application thereof

Abstract

The invention belongs to the field of gene engineering and environmental protection, and discloses a gene engineering bacterium capable of both tolerating high-concentration As (Arsenic) (III) and oxidizing As (III) and application thereof. The gene engineering bacterium is named as Pseudomonasputida AS-02 in a classified manner and preserved with a preservation number of CCTCC NO. M209215 in China Center for Type Culture Collection. The tolerance of the gene engineering bacterium to the As (III) reaches 800 mg / L, the oxidability reaches 91.7%, and the genetic stability is better; and the gene engineering bacterium can be used for treating high-concentration arsenic wastewater, and has very important environment-friendly values in reducing toxicity of the wastewater and improving a follow-up treatment effect.

Description

Technical field: [0001] The invention belongs to the fields of genetic engineering and environmental protection, and relates to a genetically engineered bacterium capable of withstanding high-concentration trivalent arsenic As(III) and efficiently oxidizing As(III) and its application. Background technique: [0002] Arsenic (As) is a carcinogen that is toxic to the human body and other organisms. Arsenic in water usually exists in an inorganic state and has two chemical valence states: trivalent arsenic As(III) and pentavalent arsenic As(V). At present, the commonly used methods for treating arsenic-containing wastewater mainly include: precipitation / co-precipitation, adsorption, ion exchange method and membrane method. However, these technologies are mainly aimed at As(V), because when the pH<9.2, the existence form of As(III) is electrically neutral, its adsorption and binding ability is weak, and the toxicity of As(III) is that of As(V) More than 100 times. Therefore...

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Problems solved by technology

[0005] Literature (Fan Qiuyan, Yang Chunyan, Xu Lin, Xu Yanhua, Construction of As(III)-resistant and efficient As(III)-oxidizing genetically engineered bacteria, Journal of Nanjing University of Technology (Natural Science Edition), 2009, 31(2): 61-64) A method for constructing As(III)-resistant and highly efficient As(III)-oxidizing genetically engineered bacteria was reported and the genetically engineered bacteria 127pBBR128-AS was obtained. Since this method uses a screening method, it cannot guarantee that the same species and the same genus can be screened out repeatedly. 1. Microorganisms with identical biochemical genetic properties do not have the practicability described in the patent law. The genetically engineered bacteria 127pBBR128-AS obtained by them have not been preserved and cannot be obtained by the public. After being transferred 30 times, the oxidized The enzyme activity is parallel to that of the original strain AS-01

[0006] At present, there is no genetically engineered strain with better stability and oxidase activity that can tolerate high concentrations of As(III) and efficiently oxidize As(III).

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