Method for extracting oligomeric proanthocyanidins from grape seeds

Abstract

The invention discloses a method for extracting oligomeric proanthocyanidins from grape seeds, and belongs to the field of the separation and purification of the oligomeric proanthocyanidins. The method comprises the following steps of: preparing extracting solution, namely crushing dried grape seeds into small particles, loading the small particles into an extraction kettle, adding hot water into the kettle for circular extraction, controlling the size of the small particles and the weight ratio of the small particles to the water, controlling the extraction pressure, temperature and time of the extraction kettle, and controlling the times of circular extraction to obtain the extracting solution; preparing pure filtrate, namely filtering the extracting solution through a filter screen to obtain crude filtrate, filtering the crude filtrate through an ultrafiltration membrane, controlling the inlet pressure and outlet pressure, temperature and flow velocity of the ultrafiltration membrane to obtain fine filtrate, performing reverse osmosis filtration on the fine filtrate, and collecting generated filtrate for recycling; and concentrating and drying, namely adding the pure filtrate into a concentration drying tank for concentration and drying, and controlling the temperature, pressure and time of concentration and drying of the concentration drying tank to obtain an oligomeric proanthocyanidins product. The method has the advantages that: extraction cost is reduced, and the safety of the product is guaranteed; energy sources are saved, and the purity and yield of the product are guaranteed; and pollution-free production and environmental protection are embodied.

Description

technical field [0001] The invention relates to a method for extracting proanthocyanidins from grape seeds, belonging to the technical field of separation and purification of proanthocyanidins . Background technique [0002] Proanthocyanidins (grape seed extract), referred to as OPC, is a bioflavonoid with a special molecular structure. It is currently recognized internationally as the most effective natural antioxidant for eliminating free radicals in the human body. It is the most powerful found so far. A free radical scavenger with very strong in vivo activity. Experiments have proved that OPC's ability to resist free radical oxidation is 50 times that of vitamin E and 20 times that of vitamin C, and it can be absorbed quickly and completely. It can reach the highest blood concentration in 20 minutes after oral administration, and its metabolic half-life is as long as 7 hours. Structurally, proanthocyanidins are composed of different amounts of catechin or epicatechin...

AI Technical Summary

Problems solved by technology

The shortcomings of the organic solvent method are: first, only the low-polymeric proanthocyanidins in the plant body can be extracted, while most of the high-polymeric proanthocyanidins remain in the plant body; second, the product purity is low, only about 50%-70%; Third, there are organic solvents remaining in the product to reduce safety, and the consumption of organic solvents is large, the cost is high, and waste water is easily generated

[0004]The second is water extraction method to extract proanthocyanidins, such as Chinese patent application number ZL02115423.6, water is selected as the extraction agent, the extraction time is long, the temperature is high, and it is easy to cause proanthocyanidin At the same time, the polarity of water is relatively large, and there are more dissolved impurities

However, the supercritical CO? extraction method is only in the laboratory stage, and its cost is high, so it is still difficult to realize industrialization

[0006] The fourth is the microwave extraction method, such as Chinese patent application number CN00120353.3, which is superior to the traditional water extraction method, but this is limited to the rough extraction stage of proanthocyanidins, and the final product purity The yield rate needs to be further studied