Bacillus cereus with higher resistance to jinggangmycin aqua and application
A technology of Bacillus cereus and Jinggangmycin, applied in the direction of application, bacteria, biocide, etc., can solve the problems of high cost and complicated operation, achieve high resistance, high survival rate, and reduce production costs
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Embodiment 1
[0051] Example 1 Bacillus cereus WR-12 isolation and identification
[0052] (1) Strain isolation: Collect soil samples from paddy fields near Tonglu County, Zhejiang Province, weigh 1 gram, and add them to a triangular flask containing 99 mL of 0.9% (m / v) sterile saline (with glass beads) , shake well, make a 1% bacterial suspension, heat in a water bath at 70°C for 10 minutes, use the flat plate coating method to isolate live bacteria, apply it to a solid plate of beef extract peptone, incubate at 30°C for 24 hours, and pick a white waxy single The colonies were isolated by streaking on the MYP identification plate, cultured at 30°C for 24 hours, picked a single milky white waxy colony with a halo and inoculated on the solid slant of beef extract peptone, cultivated at 30°C for 12 hours, and the obtained bacteria were named as cereus spores Bacillus cereus WR, its colony morphology and microscope photos are as follows figure 1 and figure 2 As shown, Bacillus cereus is an ...
Embodiment 2
[0059] The determination of embodiment 2 bacillus cereus WR-12 fermentation conditions
[0060] (1) Determination of optimum fermentation time
[0061] Inoculate Bacillus cereus WR-12 in 5 bottles of bacterial fermentation medium, pH 7.0, take 24h, 36h, 48h, 60h, and 72h as the fermentation time, respectively, and ferment at 30°C, take 5mL of fermentation broth, and centrifuge at 7000r / min for 10min. Discard the supernatant, add 5 mL of 5% Jinggangmycin aqueous solution, shake well, and place at room temperature for 8 days. Using the number of spores and the survival rate as indicators, analyze the Jinggangmycin water agent resistance of Bacillus cereus WR-12, as shown in Table 1, the results show that the optimal fermentation time of Bacillus cereus WR-12 is 48h.
[0062] Table 1 Optimum fermentation time of Bacillus cereus WR-12
[0063] serial number
Inoculation amount
fermentation time
Spore count
1
2％
24
...
Embodiment 3
[0068] Example 3 Bacillus cereus WR-12 Anti-Jinggangmycin Aqueous Characteristic Detection
[0069] (1) Comparison of spore survival rate between Bacillus cereus WR-12 and Bacillus cereus WR
[0070] Bacillus cereus WR-12 and Bacillus cereus WR were respectively inoculated in the fermentation medium, and fermented at 30° C. for 48 hours. Take 5mL of the fermentation broth, centrifuge at 7000r / min for 10min, discard the supernatant, add 5mL of 5% Jinggangmycin aqueous solution and shake well, leave it at room temperature for 8 days, use WR as a control, analyze the spore resistance of Bacillus cereus WR-12 to Jinggang In the case of aquamycin, such as image 3 As shown, the results show that the survival rate of Bacillus cereus WR-12 is about 55%, while the survival rate of Bacillus cereus WR is about 11%. The survival rate after 8 days in the water preparation has been significantly improved, reaching 5-50 times.
[0071] (2) Detection of the resistance of Bacillus cereus W...
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