Preparation method of microbial cellulose

A technology of microbial cellulose and seeds, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high price, limited application and high production cost

Inactive Publication Date: 2011-09-21
HEILONGJIANG PROV LIGHT INDAL SCI RES ACAD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Since microbial cellulose has been commercially applied in industries such as audio, food, medicine, and paper, but the current pr...

Method used

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  • Preparation method of microbial cellulose

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Embodiment Construction

[0016] Below in conjunction with specific embodiment the present invention is described in more detail:

[0017] 1.1. Source of fermentation strain

[0018] The number of Acetobacter aceti in this example is CICC21682 Acetobacter aceti, which was purchased from China Industrial Microorganism Culture Collection and Management Center.

[0019] 1.2 Strain mutagenesis

[0020] First, Acetobacter aceticum was made into bacterial suspension, and irradiated with a 15W ultraviolet lamp at a distance of 30cm for 20 seconds, stirring while irradiating, trying to make the bacteria absorb ultraviolet light waves evenly. The above irradiation process was carried out in a dark room to avoid photorepair. Transfer the bacteria after ultraviolet mutagenesis into a sterile test tube and immediately immerse in ice water for 1 hour. Under low temperature conditions, the activities of various enzymes involved in the repair of mutants in the cells are inhibited, making the repair difficult. It is...

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Abstract

The invention provides a preparation method of microbial cellulose. The method comprises the following steps of: preparing a fermentation medium, namely sterilizing 5 percent of corn hydrolyzing sugar, 0.5 percent of ammonium sulfate, 0.5 percent of NaCl, 0.2 percent of magnesium sulfate, 0.1 percent of acetic acid and 0.15 percent of lactate for 10 to 30 minutes at the temperature of between 110 and 150 DEG C, cooling the mixture to 30 DEG C, and inoculating 4 percent of ultraviolet mutagenesis acetobacter aceti seed liquor onto the mixture, and culturing the mixture for 120 hours in a shake bed; after centrifuging the fermentation liquor for 20 minutes at the speed of 3,000r/min in a high-speed centrifugal machine, pouring the supernatant fluid; heating the centrifugal precipitate for 30 minutes in a boiling water bath of which the temperature is 100 DEG C by using 4 percent of Na OH solution, to remove mycoprotein; centrifuging the solution, adding a proper amount of neutral detergent solution into the precipitate, and heating the precipitate for 1 hour in the boiling water bath of which the temperature is 100 DEG C to remove impurities, such as impure sugars, lipoids and the like from the precipitate; repeatedly washing the precipitate with deionized water and 0.5 percent of acetic acid; and drying the precipitate for 24 hours at the temperature of 80 DEG C to obtain the microbial cellulose.

Description

(1) Technical field [0001] The invention relates to a preparation method of microbial cellulose. (2) Background technology [0002] Cellulose is the most productive natural polymer on the earth, and its output reaches 100 billion tons per year. It is widely found in various higher plants, as well as in lower plants, bacteria and individual lower animals, such as several species grown in the ocean. Chlorella and some marine lower animals also contain cellulose, which exists in the form of a three-dimensional structure with hemicellulose and lignin. Microbial cellulose is different from cellulose that exists widely in nature. It exists in the form of pure cellulose and has the following characteristics: 1. The cellulose has high purity, high crystallinity and high coincidence degree, which is very beneficial in the preparation of microfiber products . 2. The fiber diameter of microbial cellulose is between 0.01-0.1 μm, the elasticity is several times to more than ten times t...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12N13/00C12N15/01C12R1/02
Inventor 陈成赵珊珊吕伟民赵云财张超
Owner HEILONGJIANG PROV LIGHT INDAL SCI RES ACAD
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