Culture solution continuous filling method and system for chlamydomonas culture
A technology for culture solution and monocytogenes, which is applied to the field of continuous injection of culture solution for the cultivation of monocysts, can solve the problems of discontinuous process, stay, and stop growth, so as to avoid the entry of external bacterial impurities, facilitate operation, and prevent The effect of leakage
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[0042] Example 1
[0043] First, configure the nutrient solution as follows:
[0044] The nutrient solution C is as follows: zinc chloride: 2g, cobalt chloride hexahydrate: 2.5g, ammonium ammonium tetrahydrate: 1.2g, copper sulfate pentahydrate: 2g, hydrochloric acid: 10 mL, dissolved in 0.1 L of deionized water; Mother liquor.
[0045] Nutrient solution A: Dissolve 2.8g ferric chloride hexahydrate, 0.5g manganese chloride hexahydrate, 27.6g boric acid, 27g sodium EDTA dihydrate, 30g sodium dihydrogen phosphate dihydrate, 90g sodium nitrate, silicon in 1L deionized water Sodium: 20 g; and add the nutrient solution C: 2 mL.
[0046] Nutrient Solution B: Vitamin B 12 :0.3 g, vitamin B 1 :23 g, vitamin H: 0.13 g, dissolved in 1L deionized water, as mother liquor.
[0047] Taking a 16L nutrient salt tank as an example, the nutrient solution A with nutrient solution C added above is added to the 16L nutrient salt tank according to the above ratio—that is, as the mother liquid into the 16L ...
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