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Sulfate reducing Citrobacter sp.strain HCSR and use thereof

A technology of Citrobacter bacillus and sulfate, applied in the field of microorganisms, can solve the problems of relatively strict sewage treatment process requirements and low bacterial concentration, and achieve the effects of improving sulfate removal capacity, rapid growth, and reducing the difficulty of treatment process.

Inactive Publication Date: 2012-11-21
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since most of the traditional SRB are strictly anaerobic bacteria, and the concentration of the bacteria is low, the requirements for the sewage treatment process are relatively strict.

Method used

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  • Sulfate reducing Citrobacter sp.strain HCSR and use thereof
  • Sulfate reducing Citrobacter sp.strain HCSR and use thereof
  • Sulfate reducing Citrobacter sp.strain HCSR and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The screening of embodiment 1 high-efficiency Citrobacter desulfurum

[0019] 1. The sample was taken from the sludge of papermaking wastewater outfall of Tianjin Paper Mill. After the sample was precipitated for 48 hours, the supernatant was taken and inserted into SRB separation and purification medium with 1% (separation and purification medium: MgCl 2 ·6H 2 O 0.4g, NH 4 Cl 1.0g, Na 2 SO 4 3.0g, Yeast extracts 1.0g, K 2 HPO 4 0.5g, CaCl 2 2H 2 O 0.05g, sodium lactate 3.0g, vitamin C (10g / L) 10mL, FeSO 4 (5g / L) 10mL. Add all ingredients to deionized water, dilute to 1L, mix well, heat to boiling, then cool to room temperature, fill with nitrogen 1×10 5 Pa sterilization, pH 7.5. The liquid medium was divided into anaerobic tubes with rubber stoppers under nitrogen and then sealed, and the solid medium was added with 1.5% agar powder. )

[0020] 2. Place in the anaerobic workstation (RUSKINN Bugbox) for enrichment, use ferrous ions as the indicator, spread t...

Embodiment 2

[0021] Physiological and biochemical identification of embodiment 2 Citrobacter HCSR

[0022] A series of character identifications are carried out to the high-efficiency sulfate-reducing bacterial strain obtained in Example 1, and the results are obtained in the following table 1:

[0023] Table 1 Morphological observation and physiological and biochemical identification results of strain HCSR

[0024]

[0025]

[0026] In this example, according to the physiological and biochemical identification method in "Bergey's Bacterial Identification Handbook" (Ninth Edition) (Baltimore: The Williams and Wilkins Co. Baltimore Md Published, May 2004), the physiological and biochemical identification of the bacterial strain HCSR was performed. , it was found that the strain was most consistent with Citrobacter werkmanii.

Embodiment 3

[0027] Example 3 16S rDNA sequence analysis of Citrobacter HCSR

[0028] The total DNA of Citrobacter HCSR cells was extracted, the primers were designed to amplify the 16S rDNA gene, and then sequenced, and the homology data were compared in the NCBI database.

[0029] 16S rDNA gene amplification and PCR product analysis template preparation: absorb an appropriate amount of bacteria and centrifuge, take out the bacteria pellet, and use a genome extraction kit (for genomic DNA extraction methods, please refer to "Bacterial Molecular Genetics Classification and Identification Method" (Shanghai Science and Technology Co., Ltd.) Publishing House, 1990) extract the total DNA of the bacterium, and the fragment size is about 20kbp. The design of the 16S rDNA gene primer adopts general primers, which are provided by Shanghai Sangong Bioengineering Technology Service Co., Ltd. The PCR reaction system is shown in Table 2, and the reaction conditions As shown in Table 3, a negative refe...

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Abstract

The invention relates to the field of microbes, in particular to a sulfate reducing Citrobacter sp.strain HCSR and use thereof. The collection number of the sulfate reducing Citrobacter sp.strain HCSR is CGMCC NO.4660. The sulfate reducing Citrobacter sp.strain HCSR screened by the invention is amphimicrobe; and compared with the conventional sulfate reducing bacteria (SRB) strict anaerobic culture condition, the strain grows quickly under an aerobic condition and has a sulfate reducing characteristic under an anaerobic condition, the SO4<2-> removing capacity is improved to 6g / L from 2.85g / Lwithin 7 days, the facultatively anaerobic physiological property of the strain is fully played, the strain is easy to culture, the difficulty in treatment process is lowered, and the strain can be widely used in the control of sulfate-containing waste water.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular, the invention relates to a Citrobacter with sulfate reduction function and application thereof. Background technique [0002] With the continuous development of industry, a large amount of sulfate (SO 4 2- ) of high-concentration organic wastewater. For this kind of wastewater, microbial anaerobic treatment methods are generally used at present, in which sulfate reducing bacteria (SRB) play an important role. The principle of microbial anaerobic treatment is mainly to use strict anaerobic sulfate reducing bacteria to reduce SO 4 2- Revert to H 2 S and other sulfides, the core catalytic function is in SRB. [0003] According to incomplete statistics, SRB has 12 genera and nearly 40 species including Desulfovibrio, Desulfotomaculum and Desulfomonas. Scholars at home and abroad have done a lot of research on sulfate-reducing bacteria, including the isolation and screening of SRB, e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/10
Inventor 邢建民周威李强李望良宋子煜王丹
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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