Livestock and poultry anti-diarrheal feed additive and preparation method thereof
A feed additive and diarrhea technology, which is applied in the field of livestock and poultry anti-diarrhea feed additive and its preparation, can solve problems such as residues and hidden dangers of food safety, and achieve the effects of mild conditions, reduction of breeding investment and improvement of drug efficacy.
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[0032] Example 1
[0033] Ingredients: 3 parts dried ginger, 2 parts woody, 6 parts licorice, 3 parts bitter wood
[0034] Preparation:
[0035] (1) Inoculate Bacillus licheniformis into a common nutrient broth medium, culture at 37℃, 200rpm shaker to produce first-grade seed liquid, when the bacterial content reaches 10 7-8 At about CFU / ml, inoculate the first-level seed liquid with the inoculum amount 5% into the second-level seed fermenter containing nutrient broth for expansion, the culture temperature is 30~32℃, the rotation speed is 200rpm, when the bacterial content reaches 10 9 When CFU / ml is about, stop fermentation and reserve;
[0036] (2) Saccharomyces cerevisiae was inoculated into the wort medium, cultured on a shaker at 28°C and 200 rpm to produce a first-grade seed liquid, when the bacterial content reached 10 7-8 At about CFU / ml, inoculate the primary seed liquid with the inoculum amount of 6% into the secondary seed fermenter containing nutrient broth to expand the cu...
Example Embodiment
[0039] Example 2
[0040] Ingredients: 5 parts of Muxiang, 3 parts of dried ginger, 8 parts of licorice, 6 parts of sorrel, 1 part of Sophorae, 5 parts of Astragalus, 5 parts of Eucommia, 3 parts of Dandelion, 2 parts of Sophora flavescens, 1 part of Turmeric, 1 part of Mutong Parts, 1 part of Atractylodes macrocephala, 2 parts of alum.
[0041] Preparation:
[0042] (1) Inoculate Bacillus subtilis into a common nutrient broth medium, culture at 37℃, 180rpm shaker to produce first-grade seed liquid, when the bacterial content reaches 10 7-8 When the CFU / ml is about, the first-level seed liquid is inoculated into the second-level seed fermentor with the inoculum amount of 3%, and the culture temperature is 35±2℃, the rotation speed is 180rpm, when the bacterial content reaches 10 9 When CFU / ml is about, stop fermentation and reserve;
[0043] (2) Lactobacillus lactis and Lactobacillus bulgaricus were respectively inoculated into MRS medium, cultured in a shaker at 37°C and 200 rpm to p...
Example Embodiment
[0046] Example 3
[0047] Ingredients: 5 parts licorice, 3 parts bitter wood, 10 parts woody, 6 parts dried ginger, 2 parts bitter beans, 3 parts astragalus, 3 parts eucommia, 4 parts dandelion, 2 parts cork, 1 part alum, 2 parts ephedra, 1 serving of medical stone
[0048] Preparation:
[0049] (1) Inoculate Bacillus licheniformis into a common nutrient broth medium, culture at 37℃, 180rpm shaker to produce first-grade seed liquid, when the bacterial content reaches 10 7-8 When CFU / ml is about, the first-level seed liquid is inoculated into the second-level seed fermenter with the inoculum amount of 3%, and the culture temperature is 32~34℃, the rotation speed is 180rpm, when the bacterial content reaches 10 9 When CFU / ml is about, stop fermentation and reserve;
[0050] (2) Inoculate Candida utilis into the wort medium, culture at 28°C and 180rpm in a shaker to produce first-grade seed liquid, when the bacterial content reaches 10 7-8 When CFU / ml is about, the first-level seed liqui...
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