Clone and expression of bovine recombinant alpha-1,3-galactoside transferase (GT)

A technology of galactoside and transferase, applied in the field of recombinant enzyme protein, can solve problems such as low activity, inability to apply clinically, and complicated procedures
CN102250852AInactive Publication Date: 2011-11-23INNER MONGOLIA MEDICAL COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INNER MONGOLIA MEDICAL COLLEGE
Publication Date
2011-11-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a recombinant zymoprotein having high activity and specific GT. According to the invention, the 80th to 368th amino acids of Inner Mongolia cow thymus GT proteins, the smallest water-soluble part with activity of GT, are employed. To further improve water-solubility and enzyme reaction activity of recombinant GT, through molecular designing and gene engineering reconstruction of natural proteins, the 160th amino acid of GT proline is modified to be lysine, the 241st amino acid of GT glutamic acid is modified to be glutamine, and therefore, recombinant zymoprotein withstronger enzymatic activity is obtained; the obtained recombinant zymoprotein has enzymatic activity 8 to 10 times that of existing zymoprotein and is applicable to the medical field of tumor treatment.
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Description

technical field

[0001] The invention relates to a highly active recombinant enzyme protein with specific galactosidyl transferase (GT) activity. The enzyme protein is a recombinant enzyme protein with stronger enzyme activity obtained through molecular design and genetic engineering of natural proteins , the recombinant enzyme protein can be applied to the medical field of treating tumors. Background technique

[0002] Organ transplantation is an important treatment technology in contemporary medicine, but the widespread development of this medical technology has been seriously affected due to the shortage of human organs. In order to find individual organs, pigs may be a good candidate for interspecific transplantation. However, if humans accept pig-derived donors, antibody-mediated hyperacute rejection is a major obstacle. The main reason for the rejection of this heterogeneous transplantation is due to the galactosyl chain on the surface of porcine vascular endothelial ...

Claims

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