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Method for preparing ellagic acid by solid fermentation with granatum as raw material

A technology of solid-state fermentation and pomegranate peel, applied in the fields of health care products and medicines, can solve the problems of high requirements on corrosion resistance of equipment, strict requirements for enzymatic conversion conditions, and difficult to control, and achieves green preparation, easy treatment, and three wastes. low pollution effect

Active Publication Date: 2011-11-23
安徽德仁生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is the process of preparing ellagic acid by acid hydrolysis (patent number: 200510002081.X), which has higher yield and purity, but requires high corrosion resistance of equipment; the other is enzymatic catalysis ( Patent No.: 200810056007.X), the main process is divided into two steps: liquid fermentation enzyme production stage and enzymatic conversion stage, the process of this method is cumbersome, and the enzymatic conversion conditions are strict and difficult to control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Solid-state fermentation of pomegranate peel

[0025] The pomegranate peel powder was placed in a fermenter, and the pomegranate peel was wetted with a 47% incipient wetness solution in which 0.1% NaCl and 0.1% MgSO were added. 4 and 0.5% NH 4 NO 3 As N source, pH 5, sterilized at 115°C for 30 minutes, after cooling, add Aspergillus niger (Aspergillus niger 3.316) spore suspension, the dosage ratio of pomegranate peel and Aspergillus niger is 5g of pomegranate peel and 1mL concentration is 1×10 10 per mL of Aspergillus niger spore suspension. Mix and culture statically, and ferment at 30°C for 5 days to obtain a fermented product containing ellagic acid.

[0026] (2) Purification of ellagic acid

[0027] After the fermentation product was dried and pulverized, 10 times the mass of pure methanol was added, and extracted twice at 70° C. for 1 hour each time. The extract was suspended and evaporated to dryness, methanol was recovered, and the obtained solid was wa...

Embodiment 2

[0031] (1) Solid-state fermentation of pomegranate peel

[0032] The pomegranate peel powder is placed in a fermenter, and the pomegranate peel is wetted with an incipient wetness solution accounting for 60% of the total weight, and 0.1% NaCl and 0.1% MgSO are added to the wetting solution 4 and 0.5% NH 4 NO 3 As N source, pH 7, sterilized at 115°C for 30min, after cooling, add Aspergillus niger (Aspergillus niger 3.316) spore suspension, the dosage ratio of pomegranate peel and Aspergillus niger is 5g pomegranate peel and 1mL concentration is 1×10 10 per mL of Aspergillus niger spore suspension. Mix and culture statically, and ferment at 30° C. for 7 days to obtain a fermented product containing ellagic acid.

[0033] (2) Purification of ellagic acid

[0034] After drying and pulverizing the fermentation product, add 30 times the mass of pure methanol and extract twice at 70°C for 3 hours each time. hours, centrifuged, and dried to obtain the ellagic acid product.

[00...

Embodiment 3

[0038] (1) Solid-state fermentation of pomegranate peel

[0039] The pomegranate peel powder is placed in a fermenter, and the pomegranate peel is wetted with an incipient wetness solution accounting for 53% of the total weight, and 0.1% NaCl and 0.1% MgSO are added to the wetting solution 4 and 0.5% NH 4 NO 3 As N source, pH 6, sterilized at 115°C for 30min, after cooling, add Aspergillus niger (Aspergillus niger 3.316) spore suspension, the dosage ratio of pomegranate peel and Aspergillus niger is 5g pomegranate peel and 1mL concentration is 1×10 10 per mL of Aspergillus niger spore suspension. Mix and culture statically, and ferment at 30° C. for 6 days to obtain a fermented product containing ellagic acid.

[0040] (2) Purification of ellagic acid

[0041] After the fermentation product was dried and pulverized, 20 times the mass of pure methanol was added, and extracted twice at 70°C for 2 hours each time. The extract was suspended and evaporated to dryness, methanol...

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Abstract

The invention discloses a method for preparing ellagic acid by solid fermentation with granatum as a raw material and belongs to the field of medicines and health-care products. In the method, solid fermentation is performed by using a culture medium which takes granatum as a main ingredient and by using Aspergillusniger 3.316 as a fermenting bacterial strain. The conditions for the solid fermentation of the granatum include: the pH value is between 5 and 7; and the granatum is initially moisturized by water by 47 to 60 percent, 0.5-percent NH4NO3 is added into a moisturizing agent as an N source, and fermentation is performed at 30 DEG C for 5 to 7 days. The fermented product is purified and dehydrated to produce the high-quality ellagic acid product. The granatum serving as a raw material is cheap, and the high yield and purity of the ellagic acid product are ensured; and the method has an outstanding cost advantage and is suitable for large-scale production.

Description

technical field [0001] The invention relates to a method for preparing ellagic acid by solid-state fermentation using pomegranate peel as a raw material, belonging to the technical field of medicines and health care products. Background technique [0002] Ellagic acid (ellagic acid), molecular formula C 14 h 6 o 8 , is a natural polyphenolic compound widely present in various nuts and soft fruits. It is a dimeric derivative of gallic acid, a polyphenolic dilactone. It can exist not only in free form, but more in condensed form (such as ellagitannin, glycoside, etc.). Pure ellagic acid is a yellow needle-like crystal, slightly soluble in water and alcohol, soluble in alkali and pyridine, and insoluble in ether. [0003] Ellagic acid has a strong ability to resist lipid peroxidation and capture free radicals, and can inhibit carcinogenesis caused by polycyclic aromatic hydrocarbons, aflatoxins and aromatic amines. Its main functions are: [0004] (1) Anti-cancer / anti-mu...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12R1/685
Inventor 袁其朋黄燕刘春国梁浩廖亮
Owner 安徽德仁生物科技有限公司
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