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Blood pressure-lowering polypeptide derived from pig blood and use thereof

A blood pressure-lowering and drug-based technology, applied in the field of biopharmaceuticals, can solve problems such as unclear active ingredients, difficulty in deep utilization, and insufficient attention to resources, and achieve good market prospects

Inactive Publication Date: 2011-12-14
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Similar to my country's historical background, Japan's development and utilization rate of pig blood is about 50-60% (mainly according to the records of Materia Medica, it is developed into medicine after deep processing with modern biotechnology), and the main reason is that the country does not pay enough attention to this resource , the active ingredient is not clear, it is difficult to use deeply

Method used

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  • Blood pressure-lowering polypeptide derived from pig blood and use thereof
  • Blood pressure-lowering polypeptide derived from pig blood and use thereof
  • Blood pressure-lowering polypeptide derived from pig blood and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 In vitro enzymatic hydrolysis of pig blood simulating in vivo metabolism

[0034] The main component of pig blood after drying is protein, with a content of nearly 20%, among which hemoglobin in blood cells accounts for 75% of the total protein in whole blood. After hemoglobin is hydrolyzed, it is degraded into small molecule peptide compounds with different chain lengths. The main color and odor substance in pig blood is heme, which seriously interferes with the extraction and separation of effective substances, content determination, molecular weight detection and other research. When pig blood enters the human body, it is first degraded by digestive enzymes into relatively stable peptides (molecular weight range is 0.1kDa ~ 10kDa). Small peptides with a molecular weight of less than 3kDa are usually biologically active, and these small peptides are also easily absorbed by the human body. This kind of small peptide is also a common substance for the effect ...

Embodiment 2

[0113] Example 2 Separation and identification of pig blood blood pressure-lowering functional polypeptide

[0114] The pathogenesis of hypertension is generally considered to be that angiotensinase (ACE) excessively converts angiotensin I (Ang I) into angiotensin II (Ang II) that raises blood pressure, and at the same time, ACE degrades bradykinin, making it inactive. live. Therefore, by inhibiting the activity of ACE, the production of angiotensin II is reduced, and the degradation (inactivation) of bradykinin is resisted, thereby lowering blood pressure. Cushman first proposed ACE from rabbit lung, and established a method for measuring ACE inhibitory activity. Later, many researchers made changes on this basis. In this experiment, the antihypertensive functional polypeptide of pig blood was screened out by simulating the inhibitory effect on ACE in vivo in vitro.

[0115] In this experiment, after separation by gel filtration, RP-HPLC was used for fine separation. The se...

Embodiment 3

[0166] Example 3 Pharmacological Verification of Antihypertensive Peptide

[0167] The antihypertensive peptides with ACE inhibitory activity confirmed by in vitro experiments do not necessarily all have antihypertensive effect in vivo. Antihypertensive peptides must enter the blood circulation to exert their antihypertensive effect. If taken orally, they are easily decomposed by enzymes in the digestive tract or degraded by ACE first, and may become inactive short peptides or amino acids. Can the antihypertensive peptide obtained in the present invention successfully pass through the barrier of the gastrointestinal tract? Through in vivo experiments such as animal experiments or clinical trials, the effect of the hypotensive peptide can be more accurately judged.

[0168] In previous experiments, 6 hypotensive peptides with ACE inhibitory activity have been obtained. In order to further verify the actual antihypertensive effect of these antihypertensive peptides, this exper...

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Abstract

The invention belongs to the field of biological pharmacy, particularly relates to blood pressure lowering functional polypeptide from pig blood and application thereof, and aims to solve the technical problem of providing new blood pressure lowering functional polypeptide. The technical scheme for solving the technical problem is to provide the blood pressure lowering functional polypeptide frompig blood. The blood pressure lowering functional polypeptide is (1) polypeptide with an amino acid sequence shown as SEQ ID No. 6 or (2) polypeptide with the same or similar function formed by substituting, deleting or adding one or more amino acids on the basis of the amino acid sequence shown as the SEQ ID No. 6. In-vivo and In-vitro tests prove that the polypeptide has a good blood pressure lowering effect, can be prepared into a blood pressure lowering medicament and provides a new effective choice for the field of blood pressure lowering.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to a blood pressure-lowering polypeptide derived from pig blood and its application. Background technique [0002] Pig blood has a long history of medicinal use. Since the Han Dynasty, the herbal medicines of all dynasties have listed pig blood separately, and recorded its medicinal effects: "replenishing blood and nourishing the heart, calming wind and calming convulsions, lowering qi, and stopping bleeding" and so on; At the same time, a large number of proven prescriptions are also included. Folks also widely consume pig blood to prevent and treat diseases. Modern research also shows that pig blood not only contains 18 kinds of amino acids including 8 kinds of amino acids necessary for the human body, but also has high nutritional value. It can also treat anemia, leukemia, reduce blood lipids, and promote wound healing. However, at present, people mainly use the ed...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12N15/12C12N15/63A61K38/08A61P9/12
Inventor 国锦琳任艳万德光
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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