Rice Metal Tolerance Protein osmpt1 and its Encoding Gene and RNA Interference Fragment

Abstract

The invention discloses rice metal-tolerant protein OsMPT1 as well as an encoding gene and an RNA (Ribonucleic Acid) interference segment thereof. The amino-acid sequence of the rice metal-tolerant protein OsMPT1 is shown as SEQ ID NO.2, the nucleotide sequence of the encoding gene OsMPT1 is shown as SEQ ID NO.1, and the gene is a key gene for responding cadmium stress, which has important significance for comprehensively understanding the biological function of metal-ion transport protein in a plant. The nucleotide sequence of the RNA interference segment for the rice metal-tolerant protein OsMPT1 is shown as SEQ ID NO.3, the expressing quantity of the OsMPT1 gene in the whole rice plant can be decreased after the RNA interference segment is expressed in rice by utilizing an expressing vector, and the heavy-metal cadmium-enriching quantity of rice seeds is also decreased along with the decrease of the expressing quantity of the OsMPT1 gene. The RNA interference segment for the rice metal-tolerant protein OsMPT1 disclosed by the invention can be applied to the gene-engineering inheritance breeding of the rice, rice varieties with low-absorption enriched cadmium are cultivated, andfood safety hazards brought to grain crops by heavy-metal cadmium are lightened.

Description

Technical field: [0001] The invention belongs to the field of biogenetic engineering, and in particular relates to rice metal tolerance protein OsMPT1, its coding gene and its RNA interference fragment. Background technique: [0002] Rice is one of the most important food crops in the world and the largest food crop in my country. The discharge of industrial "three wastes" has caused huge environmental pollution, especially the problem of heavy metal pollution has become increasingly serious, and has become one of the important problems that endanger human society. In areas with serious heavy metal pollution in my country, such as the Pearl River Delta, the Yangtze River Delta and other highly industrialized areas, as well as parts of central my country where the mining industry is developed (such as Chenzhou, Hengyang, Yongzhou, Changde, Zhuzhou, etc. in Hunan), all are Rice is the main food crop, and the rice produced in these areas often has excessive levels of heavy meta...

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Problems solved by technology

However, Arabidopsis halleri's close relative species, Arabidopsis lyrata and Arabidopsis thaliana, cannot tolerate and accumulate heavy metals. The genomes of these two plants only contain one copy of the MTP1 gene, and its expression level is also low ( DB et al.Two genes encoding Arabidopsis halleri MTP1 metal transport proteins co-segregate with zinc tolerance and account for high MTP1 transcript levels.Plant J.2004.39(3):425-39)

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