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A strain of Bacillus pumilus and its application in disease prevention and growth promotion

A technology of Bacillus pumilus and fungi, applied in bacterial diseases and growth promotion, in the field of tobacco fungus control, to achieve broad application prospects, good control effect and growth promotion effect, and excellent growth promotion effect.

Active Publication Date: 2011-12-28
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been reports on the biological control of tobacco fungi and bacterial diseases in China, but most of the bio-control strains only have a good control effect on a certain disease, and the Bacillus strains that have bio-control effects on various fungi and bacterial disease pathogens are domestic Few studies and reports

Method used

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  • A strain of Bacillus pumilus and its application in disease prevention and growth promotion
  • A strain of Bacillus pumilus and its application in disease prevention and growth promotion
  • A strain of Bacillus pumilus and its application in disease prevention and growth promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Antagonism of AR03 and its fermented liquid on tobacco main fungi and bacterial disease pathogens

[0033] Inoculate AR03 on the NA plate and culture at 30°C for 48 hours, then use the plate confrontation method, that is, inoculate the bacteria cakes (3mm in diameter) of black shank bacteria, Alternaria rubella, and anthracnose bacteria on the oat plate, pick AR03 with the inoculation loop and use the bacteria cakes Draw a line equidistant from the center, culture at 30°C for 5 days, and use the plate diffusion method to inhibit R. solanacearum; observe the inhibition of AR03 on the above-mentioned pathogenic bacteria. From indoor antibacterial effect of plate, AR03 thallus all has very good inhibitory action to tobacco black shank bacterium, red spot bacterium, anthracnose bacterium and Ralstia solanacearum (see figure 1 , 4 , 7 and 8).

[0034] The antagonistic activity of the fermentation broth of the AR03 strain was obtained by inoculating the activated...

Embodiment 2

[0035] Example 2 Pot control effect of AR03 fermented liquid on tobacco black shank

[0036] Pot control effect of AR03 fermented liquid on tobacco black shank and bacterial wilt: use AR03 fermented liquid to irrigate the roots to treat tobacco seedlings, the specific method is as follows: insert the activated AR03 strain into NB medium, shake the table for 48 hours After that, dilute 100 times (the concentration of the culture solution is 1*10 8 cfu / ml), spare. Preparation of the black shank fungus fungus: after the black shank is activated, insert it into a triangular flask equipped with millet medium, and cultivate it at a constant temperature (28°C) for 8 days, that is, after the black shank is overgrown with the millet medium, take it out from the triangular flask, grind Crushed to get fungus. The tobacco variety tested was Xiaojin 1025. Tobacco seedlings with 3-4 leaves were transplanted into flower pots, and the roots were irrigated with AR03 culture solution, 50ml pe...

Embodiment 3

[0039] Embodiment 3 AR03 bacterial strain is to the inhibitory action of black shank bacterium, red spot fungus mycelia growth

[0040] With blackleg and Alternaria rubella as target bacteria, AR03 was inoculated on the NA plate and cultured at 30°C for 48 hours, and then plate confrontation method was adopted, that is, blackleg and Alternaria rubella were inoculated on the oat plate, and AR03 was picked with an inoculation loop and injected with The fungus cake was drawn as the center at equal distances, placed at 30°C for 5 days, and the hyphae at the edge of the fungus cakes of black shank bacteria and Alternaria rubella were respectively picked and inspected under a microscope (see image 3 and Image 6 ), the results showed that compared with the normal hyphae, the cytoplasm of the black shank bacteria cake edge hyphae treated with AR03 was aggregated into a spherical shape, separated from the cell membrane, and could not grow normally; while the red spot bacteria cake ed...

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Abstract

The invention discloses a Bacillus pumilus. The Bacillus pumilus was preserved in China General Microbiological Culture Collection Center on August 23, 2010, and its preservation number is CGMCC NO.4117. The Bacillus pumilus disclosed in the invention can be used for preparing bactericides for biological control of tobacco or vegetable fungus diseases, and can be used for preparing growth promoting agents of tobacco or vegetable for promoting the growth of plants, especially tabocco. The bacterial strain has good stability, strong stress resistance, and excellent effect of promoting growth, is an excellent microbial pesticide and an excellent microbial pesticide resource. The bacterial strain has a wide application prospect.

Description

technical field [0001] The invention relates to a strain of bacillus pumilus and its application in preventing and treating tobacco fungus and bacterial diseases and promoting growth. Background technique [0002] Tobacco is often harmed by multiple fungal diseases from seedling cultivation to harvest. There are more than 60 kinds of tobacco fungal and bacterial diseases recorded in China, of which nearly 10 are serious, including tobacco black shank, tobacco bacterial wilt, red spot disease , Anthracnose, black disease and powdery mildew. Some pathogens can harm tobacco at various growth stages, and some pathogens only harm certain organs during a certain growth stage of tobacco; in addition, some tobacco diseases such as tobacco powdery Black shank, tobacco red spot and tobacco anthracnose have almost no regional restrictions, and often occur with the climate of different years. With the continuous development of tobacco specialized production, the requirements for quali...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00A01P21/00C12R1/07
Inventor 王静孔凡玉张成省
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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