Enzymolysis-chemistry combined method for producing sodium alginate
A technology of sodium alginate and combined method, applied in the field of seaweed chemical industry, can solve the problems of environmental pollution, difficult treatment, high cost, etc., achieve the effects of reducing acid-base wastewater, easy control of production process, and reducing coal consumption
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[0031] Example 1
[0032] Weigh 10 kilograms of brown algae such as dried kelp, leaf algae, blackened thunderbolt or bark algae, add 50 liters of tap water and soak for 1 hour to remove the sediment. Add 50 liters of tap water and soak for 3 hours and filter. Cut the brown algae into 20cm-long pieces with a chopper, transfer to a 50L beater and add 20 liters of tap water to stir and beat for 0.5h. Transfer the slurry to a 50L enzymatic hydrolysis tank with insulation and heating function, and transfer the compound enzyme that has been configured according to the ratio to the enzymatic hydrolysis tank. The enzyme dosage is 148u / g, at a temperature of 45°C, pH4 .5. Carry out incubation and enzymatic hydrolysis under the condition of 13h. After enzymolysis, the temperature was raised to 90°C and kept for 0.5h to inactivate the enzyme. The enzymatic hydrolysate is separated by filtration, and the filter residue is retained for later use. Add 100 mesh 1.0 kg of chemically pure anh...
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[0034] Example 2
[0035] Weigh 10 kilograms of dried brown algae such as Ascophyllum vulgaris, Lethosporium blackenedi, Bark algae, or light-dried kelp, and add 50 liters of tap water to soak for 1.5 hours to remove the sediment. Then add 50 liters of tap water to soak for 3.5 hours and filter. Cut the brown algae into 15cm-long pieces with a chopper, transfer to a 50L beater and add 20 liters of tap water to stir and beat for 1.5h. Transfer the slurry to a 50L enzymatic hydrolysis tank with heat preservation and heating functions, and add the compound enzymes that have been configured according to the ratio into the enzymatic hydrolysis tank. The enzyme dosage is about 300u / g, at a temperature of 55°C, pH5 .0, incubate enzymatic hydrolysis under the condition of 25h action time. After enzymolysis, the temperature was raised to 85°C and kept for 45 minutes to inactivate the enzyme. The enzymatic hydrolysate is separated by filtration and the filter residue is retained. Add 0...
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[0038] Example 3
[0039] The difference from Example 1 is:
[0040] The compound enzyme consists of 6ml of liquid cellulase (C11840 imported from the United States 270,000 u / ml), 4.5g of refined solid pectinase (P-3028, 500,000 u / ml from Sigma, USA) and hydrolyzed plant protease (F-3925, American Sigma company, 100,000 u / g) 1.5g composition. 4.02 million u
[0041] Weigh 10 kilograms of brown algae such as dried Ascophyllum vulgaris, Blackened thunderbolt, Bark algae, or light-dried kelp, and add 50 liters of tap water to soak for 2.0 hours to remove the sediment. Then add 50 liters of tap water to soak for 4.0 hours and filter. Cut the brown algae into 15cm long pieces with a chopper, transfer to a 50L beater and add 20 liters of tap water to stir and beat for 1.8h. Transfer the slurry to a 50L enzymatic hydrolysis tank with heat preservation and heating function, and add the compound enzymes that have been configured according to the ratio into the enzymatic hydrolysis tank. T...
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