Recombination yeast engineering bacteria expressing reticuloendotheliosis virus (REV) Gp90 protein, its construction method and application

Abstract

The invention discloses a recombination yeast engineering bacteria expressing reticuloendotheliosis virus Gp90 protein, its construction method and application. The recombination yeast engineering bacterial strain is preserved in CGMCC with the preservation number CGMCC No. 5046. With large scale induction of the engineering bacterial strain, the expression of Gp90 protein is 373 mg / L. The SDS-PAGE and Western blot detections prove that expressed recombination protein has biological activity and antigenicity of reticuloendotheliosis virus natural protein. By preparing an antigen vaccine from the recombination protein to immunize SPF chickens, the results show that: the recombination antigen vaccine can effectively induce the organism to generate specific humoral immune response to preventthe SPF chickens from attack of anti-REV, and can effectively prevent the propagation of the virus in body.

Description

technical field [0001] The present invention relates to a recombinant yeast genetically engineered strain constructed by genetic engineering technology, in particular to a recombinant yeast engineered strain expressing the Gp90 protein of avian reticuloendotheliosis virus, and also to its construction method, and further to the expressed recombinant yeast strain Protein and its application in the development of a new type of recombinant antigen vaccine against avian reticuloendotheliosis virus. The invention belongs to the field of biomedicine. Background technique [0002] Avian reticuloendotheliosis is caused by the infection of poultry reticuloendotheliosis virus (REV). It is an important viral disease that can lead to immunosuppression of chickens, and seriously endangers the poultry industry in the world. With the new changes of the disease in recent years, such as the increase in the age of onset, the increase in the mortality rate after infection, the severity of mix...

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Problems solved by technology

[0003] The REV genome encodes three proteins, Gag, Pol, and Env. The envelope protein env is further divided into two proteins, Gp90 and Gp20. Among them, the Gp90 protein plays an important role in the virus stimulating the body to produce neutralizing antibodies. People use Escherichia coli prokaryotic expression system and Eukaryotic expression systems such as baculovirus have expressed the Gp90 gene in vitro, but because the expression product either cannot stimulate the body to produce neutralizing antibodies, or the protein expression level is low, multiple booster immunizations are required to produce high-titer antibodies. Therefore, From an economic point of view, it is not suitable for large-scale commercial production

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