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Method for producing cordyceps sinensis hyphae by virtue of solid-state fermentation

A Cordyceps mycelia and solid-state fermentation technology, which is applied in botany equipment and methods, fertilizer mixtures, horticulture, etc., can solve the shortage of medium substrate and fermentation method for the production of Cordyceps fungus powder, and the artificial cultivation technology of Cordyceps remains in the laboratory stage. The long growth cycle of solid cultivation technology can achieve the effect of shortening the cultivation cycle, reducing the pollution of bacteria and shortening the cycle

Active Publication Date: 2012-02-08
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the special requirements of growing geography and strict parasitism, its resources are extremely scarce, and it is not easy to harvest and the wild environment is constantly being destroyed. The expansion and years of excessive mining and digging, Cordyceps sinensis is now in an extremely endangered situation, which greatly limits their application, and artificial cultivation can make up for the existing problems
[0004] At present, the artificial cultivation methods of Cordyceps sinensis are mainly solid cultivation and liquid submerged fermentation, however, there are few reports about the artificial cultivation methods through solid fermentation cultivation
Since the 1970s, many research institutions have carried out research on the artificial cultivation of Cordyceps sinensis. However, more than 40 years have passed, and the artificial cultivation technology of Cordyceps sinensis is still in the laboratory stage, and has not yet achieved large-scale commercialization.
Chinese patent CN201010214791.X (Cultivation method of Cordyceps sinensis mycelium and fruiting body) discloses a cultivation method of Cordyceps sinensis mycelium and fruiting body. This solid cultivation technology has a long growth cycle, is greatly affected by environmental conditions, and is difficult to scale production
[0006] Searched through the patent database, there are very few patents related to the cultivation of Cordyceps sinensis by solid fermentation. method, but there are obvious deficiencies in the medium substrate and fermentation method used in the production of Cordyceps sinensis powder
CN1650686 adopts solid culture method, is to cultivate militaris militaris in glass bottles, and large-scale production is affected

Method used

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Examples

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Embodiment 1

[0031] Example 1: The method for producing Cordyceps sinensis mycelium by solid-state fermentation comprises the following steps:

[0032] A, preparation of Cordyceps sinensis first-class slant seeds: first prepare the slant PDA culture medium, inoculate the strains into the PDA solid slant after sterilization, and cultivate at a certain temperature to obtain the first-class slant seeds;

[0033] B. Prepare Cordyceps sinensis second-level triangular flask liquid seeds: first prepare the shaker flask liquid seed medium, mix and sterilize, insert the first-level slant seeds in step A after cooling, and cultivate for a certain period of time in a shaking table to obtain second-level triangular flask liquid seeds ;

[0034] C. Solid-state culture of fungal culture bags: the solid-state culture medium is based on wheat bran. First, according to the formula of the liquid seed culture medium in the above-mentioned step B, sucrose, yeast extract powder, potassium dihydrogen phosphat...

Embodiment 2

[0037] Example 2: Different from Example 1, the formula of described slant PDA medium comprises: 15% of potato dipping juice, 15 g / L of sucrose, agar 1.5% (w / v), pH=5.8; After sterilization, then The bacteria were inoculated into the PDA solid slant and cultured statically at 22 °C for 110 h.

Embodiment 3

[0038] Example 3: Different from Example 1, the formula of the described slant PDA medium comprises: 25% of potato dipping juice, 25 g / L of sucrose, 2.3% (w / v) of agar, pH=6.5; After sterilization, then The strains were inoculated into the PDA solid slant and cultured statically at 30 °C for 130 h.

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Abstract

The invention relates to a method for producing cordyceps sinensis hyphae by virtue of solid-state fermentation. The method comprises the following steps of: A, preparing first-grade slant seeds of cordyceps sinensis; B, preparing second-grade triangular-flask liquid-state seeds of the cordyceps sinensis; C, carrying out solid-state culture in a fungus culture bag; and D, collecting the cordycepssinensis hyphae. The method provided by the invention has the advantages that the production cycle is short, the cost is low and the quality is good; the cordyceps sinensis hyphae are cultured in thefungus culture bag so that the operation is simple; and the cordyceps sinensis is cultured by use of agricultural and sideline products so that the method is convenient for realization of large-scaleproduction.

Description

technical field [0001] The invention relates to a method for producing Cordyceps sinensis mycelia by solid-state fermentation, and belongs to the technical field of microbial fermentation. Background technique [0002] Cordyceps sinensis belongs to Ascomycetes, Ergotaceae ( Clavicipitaceue ), Cordyceps ( Cordyceps ) is a combination of fungi parasitic on the larvae of the bat moth family, and is a precious medicinal fungus. In recent years, it has been proved by medical research that Cordyceps sinensis has the function of regulating the metabolism and immune system of the human body, and can be used for many diseases of the human body system. Cordyceps sinensis contains a variety of active ingredients, such as polysaccharides, nucleosides, cordycepin, sterols, polypeptides, etc. [0003] Due to the special requirements of growing geography and strict parasitism, its resources are extremely scarce, and it is not easy to harvest and the wild environment is constantly being...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 梁运祥涂飞赵述淼葛向阳彭楠王绩王玢
Owner HUAZHONG AGRI UNIV
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