Coriobacterium sp. AUH-Julong21 and use of coriobacterium sp. AUH-Julong21 in liquiritigenin conversion
An auh-julong21, Antarctica technology, applied in bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as lack of DG resources
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Embodiment 1
[0050] 1. Isolation and cultivation of strain Julong21
[0051] (1) Collection and culture of human feces samples
[0052] Use a sterilized cotton swab to pick up fresh feces, put it into 1 ml of fresh BHI liquid medium, place it in an anaerobic workstation and cultivate it at 37°C for 24 hours, as a microbial flora for screening specific functional microbial strains;
[0053] (2) Isolation and cultivation of Red Toon fungus AUH-Julong21
[0054] ①Single colony isolation culture
[0055] Use fresh BHI liquid medium to serially dilute the microbial flora that has been cultivated in the anaerobic workstation for 24 hours, and dilute to a concentration of 10 –1 , 10 –2 、10 –3 、10 –4 、10 –5 、10 –6 、10 –7 、10 –8 , and then 100 microliters of concentration were respectively 10 –5 、10 –6 、10 –7 、10 –8 The dilution of microbial flora was uniformly coated on the pre-prepared BHI solid medium, and the BHI solid medium coated with the dilution of microbial flora was placed i...
Embodiment 2
[0084] The isolation method of bacterial strain Julong21 is the same as that in Example 1.
[0085] In the anaerobic workstation, the seed liquid of the above-mentioned pre-cultivated strain Julong21 was transferred to a 250-milliliter Erlenmeyer flask filled with 100 milliliters of liquid medium by 10% inoculum for cultivation, and at the same time, 40 mmol / L of crude glycyrrhizin was added. 0.5 ml (the concentration of crude glycyrrhizinin is calculated based on the peak area value of liquiritinin in crude glycyrrhizin and the standard curve of pure glycyrrhizinin), cultured in an anaerobic workstation for 2 days. Use an equal volume of ethyl acetate to extract the culture in the Erlenmeyer flask twice, filter the extract and evaporate to dryness on a rotary evaporator, then add 100% methanol solution, pass through an organic membrane with a pore size of 0.45 microns, and use a preparative column to perform HPLC analysis. Separation and preparation were carried out on the ab...
Embodiment 3
[0087] The isolation method of bacterial strain Julong21 is the same as that in Example 1.
[0088] In the anaerobic workstation, the seed liquid of the above-mentioned pre-cultivated strain Julong21 was transferred to a 250-milliliter Erlenmeyer flask filled with 100 milliliters of liquid medium by 10% inoculum for cultivation, and at the same time, 60 mmol / L of crude glycyrrhizin was added. 1.3 ml (the concentration of crude glycyrrhizinin is calculated based on the peak area value of liquiritinin in crude glycyrrhizin and the standard curve of pure glycyrrhizinin), cultured in an anaerobic workstation for 3 days. Use an equal volume of ethyl acetate to extract the culture in the Erlenmeyer flask twice, filter the extract and evaporate to dryness on a rotary evaporator, then add 100% methanol solution, pass through an organic membrane with a pore size of 0.45 microns, and use a preparative column to perform HPLC analysis. Separation and preparation were carried out on the ab...
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