Rapeseed peptide for reducing blood pressure, preparation method and applications thereof

A technology for lowering blood pressure and rapeseed is applied in the field of preparation of bioactive peptides, which can solve the problems of small scope of action of a single enzyme, low degree of hydrolysis and low degree of hydrolysis, and achieves abundant low molecular weight peptides, mild separation conditions, and improved degree of hydrolysis. Effect

Inactive Publication Date: 2012-03-28
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Alcalase Alcalase (NOVO Company, Denmark) is a non-specific protein peptide bond endonuclease with abundant sources and strong enzymatic hydrolysis ability. High, the molecular weight of the obtained polypeptide is relatively large
Moreover, the hydrophobic amino acid residues of rapeseed protein are exposed after enzymatic hydrolysis, and the obtained rapeseed polypeptide has a distinct bitter taste. In the later stage, a cumbersome debittering process is required, which requires high energy consumption and high cost,...

Method used

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  • Rapeseed peptide for reducing blood pressure, preparation method and applications thereof
  • Rapeseed peptide for reducing blood pressure, preparation method and applications thereof
  • Rapeseed peptide for reducing blood pressure, preparation method and applications thereof

Examples

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Effect test

Embodiment 1

[0021] A preparation method of hypotensive rapeseed peptide, comprising the steps of:

[0022] (1) Mix the degreased rapeseed cake with water in a mass ratio of 1:10, adjust the pH to 8.3 with NaOH aqueous solution, stir and extract for 1 hour, centrifuge at 4000r / min for 20 minutes, and settle according to the solid-liquid mass ratio. Extract twice at a ratio of 1:5, combine the supernatant, adjust pH=5.0 with HCl aqueous solution to precipitate protein, separate the supernatant, adjust the supernatant again with HCl aqueous solution to pH=3.7 for secondary protein precipitation, discard The supernatant was combined with two precipitates, washed with deionized water for 3 times, adjusted to pH = 7.0 with NaOH aqueous solution, stirred to redissolve the precipitate, and freeze-dried for 24 hours to obtain rapeseed albumin;

[0023] (2) dissolving the rapeseed albumin in distilled water to make an aqueous solution of rapeseed albumin with a mass concentration of 4.87%; the rati...

Embodiment 2

[0028] A preparation method of hypotensive rapeseed peptide, comprising the steps of:

[0029] (1) Mix the degreased rapeseed cake with water at a mass ratio of 1:8, adjust the pH to 8.3 with NaOH aqueous solution, stir and extract for 1.5 h, centrifuge at 5000 r / min for 15 min, and settle according to the solid-liquid mass ratio Extract 3 times at a ratio of 1:3, combine the supernatant, adjust pH=5.0 with HCl aqueous solution to precipitate protein, separate the supernatant, adjust the supernatant again with HCl aqueous solution to pH=3.7 for secondary protein precipitation, discard For the supernatant, combine the two precipitates, wash the precipitate twice with deionized water, adjust the pH to 7.0 with NaOH aqueous solution, stir to redissolve the precipitate, and freeze-dry for 24 hours to obtain rapeseed albumin;

[0030] (2) dissolving the rapeseed albumin in distilled water to make a rapeseed albumin aqueous solution with a mass concentration of 3%; the ratio of the ...

Embodiment 3

[0033] A preparation method of hypotensive rapeseed peptide, comprising the steps of:

[0034] (1) Mix the degreased rapeseed cake with water at a mass ratio of 1:15, adjust the pH to 8.3 with NaOH aqueous solution, stir and extract for 0.5h, centrifuge at 3500r / min for 25min, and settle according to the solid-liquid mass ratio Extract twice at a ratio of 1:7, combine the supernatant, adjust the pH=5.0 with HCl aqueous solution to precipitate the protein, separate the supernatant, adjust the supernatant again with the HCl aqueous solution to pH=3.7 to precipitate the protein again, discard In the supernatant, combine the two precipitates, wash the precipitate three times with deionized water, adjust the pH to 7.0 with NaOH aqueous solution, stir to redissolve the precipitate, and freeze-dry for 24 hours to obtain rapeseed albumin;

[0035](2) dissolving the rapeseed albumin in distilled water to make a rapeseed albumin aqueous solution with a mass concentration of 5%; the rati...

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PUM

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Abstract

The invention discloses a rapeseed peptide for reducing blood pressure, preparation method and applications thereof. The preparation method is as follows: firstly, mixing degreased rapeseed cake with water, adjusting pH value, extracting, centrifuging, settling, extracting again, combining supernatant, adjusting the pH value to settling protein, separating the supernatant, adjusting the pH value of the supernatant to conduct the secondary protein settlement, abandoning the supernatant, combining the settlement, washing the settlement by deionized water, adjusting the pH value, stirring so as to dissolve the settlement, and then cooling and drying, thus obtaining rapeseed protein; and secondly, dissolving the rapeseed protein in distilled water, adding alcalase for hydrolysis, then adding flavourzyme for hydrolysis, killing enzyme, cooling, then obtaining the supernatant by centrifuging, cooling and drying the supernatant, thus obtaining the rapeseed peptide for reducing the blood pressure. The invention has the following advantages: the alcalase and the flavourzyme are utilized for hydrolysis, the hydrolysis degree is improved effectively, the bitterness of the rapeseed peptide is reduced, simultaneously, the rapeseed peptide with richer low molecular weight peptide and blood pressure reducing activity can be obtained. Furthermore, the condition is moderate, the flow is simple, the energy consumption is low, and the industrial production is easy.

Description

technical field [0001] The invention belongs to the technical field of preparation of bioactive peptides. Background technique [0002] Rapeseed is one of the important oil crops in our country. Rapeseed protein contained in rapeseed cake is a very good protein and a huge vegetable protein resource. Albumin and gluten were the main protein isolates in Huaza No. 3 rapeseed cake, accounting for 36.8% and 31.6% of the total protein isolates, respectively. The others are gluten (29.1%) and gliadin (2.5%) in turn. Amino acid detection showed that rapeseed protein isolate was rich and balanced in amino acid content, the essential amino acid content was close to 50%, and it was rich in limiting amino acids such as lysine and methionine. Since the selected cake is a low-temperature cold-rolled raw material, the protein is not denatured by heat during processing, so the inherent properties of the protein are maintained, and it can be widely used in food and beverages. [0003] Wit...

Claims

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Application Information

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IPC IPC(8): A23J3/14A23J3/34A23L1/305
Inventor 薛照辉寇晓虹高捷汪一帆周凤娟
Owner TIANJIN UNIV
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